Shining light on Drosophila oogenesis: live imaging of egg development

He, Li; Wang, Xiaobo; Montell, Denise J.

doi:10.1016/j.gde.2011.08.011

Cited by 53 publications

(47 citation statements)

References 69 publications

(70 reference statements)

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“…S10). From the border region, it takes approximately 5 d for the completion of oogenesis (36). Compared with the previous 2.5 d of cyst development in regions 1 and 2A, where there is the potential for Wolbachia transmission from the GSCN and escort cells, the developmental time available for transmission of Wolbachia derived from the SSCN is about twice as long (Fig.…”

Section: Discussionmentioning

confidence: 84%

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Evolutionarily conserved Wolbachia -encoded factors control pattern of stem-cell niche tropism in Drosophila ovaries and favor infection

Toomey

Panaram²,

Fast³

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

110

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Wolbachia are intracellular bacteria that infect invertebrates at pandemic levels, including insect vectors of devastating infectious diseases. Although Wolbachia are providing novel strategies for the control of several human pathogens, the processes underlying Wolbachia's successful propagation within and across species remain elusive. Wolbachia are mainly vertically transmitted; however, there is also evidence of extensive horizontal transmission. Here, we provide several lines of evidence supporting Wolbachia's targeting of ovarian stem cell niches-referred to as "niche tropism"-as a previously overlooked strategy for Wolbachia thriving in nature. Niche tropism is pervasive in Wolbachia infecting the Drosophila genus, and different patterns of niche tropism are evolutionarily conserved. Phylogenetic analysis, confirmed by hybrid introgression and transinfection experiments, demonstrates that bacterial factors are the major determinants of differential patterns of niche tropism. Furthermore, bacterial load is increased in germ-line cells passing through infected niches, supporting previous suggestions of a contribution of Wolbachia from stem-cell niches toward vertical transmission. These results support the role of stem-cell niches as a key component for the spreading of Wolbachia in the Drosophila genus and provide mechanistic insights into this unique tissue tropism.endosymbiont | maternal transmission | microbial tissue tropism | germline stem cell niche | somatic stem cell niche

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Section: Discussionmentioning

confidence: 84%

“…Our current understanding of the mechanisms involved in Wolbachia localization is limited (36). Toward dissecting the mechanistic basis of stem-cell niche tropism, we investigated the relative role of bacterial versus host factors in the different patterns of niche tropism.…”

Section: Discussionmentioning

confidence: 99%

Evolutionarily conserved Wolbachia -encoded factors control pattern of stem-cell niche tropism in Drosophila ovaries and favor infection

Toomey

Panaram²,

Fast³

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

110

View full text Add to dashboard Cite

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“…The newly eclosed females were collected and fed with yeast powder at 25°C for 36 hours (He et al, 2011), and then the composition of each ovariole with different stages of developing egg chambers was analyzed. Ovarioles from OreR females were classified in the following two groups: one group contained at least one middle stage egg chamber (vitellogenic stage 8-10) and one late stage egg chamber (Fig.…”

Section: Fig 4 Ectopically Expressing Oskar Recruits D-endob To Thementioning

confidence: 99%

Endophilin B is required for the Drosophila oocyte to endocytose yolk downstream of Oskar

et al. 2014

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The nutritional environment is crucial for Drosophila oogenesis in terms of controlling hormonal conditions that regulate yolk production and the progress of vitellogenesis. Here, we discovered that Drosophila Endophilin B (D-EndoB), a member of the endophilin family, is required for yolk endocytosis as it regulates membrane dynamics in developing egg chambers. Loss of D-EndoB leads to yolk content reduction, similar to that seen in yolkless mutants, and also causes poor fecundity. In addition, mutant egg chambers exhibit an arrest at the previtellogenic stage. D-EndoB displayed a crescent localization at the oocyte posterior pole in an Oskar-dependent manner; however, it did not contribute to pole plasm assembly. DEndoB was found to partially colocalize with Long Oskar and Yolkless at the endocytic membranes in ultrastructure analysis. Using an FM4-64 dye incorporation assay, D-EndoB was also found to promote endocytosis in the oocyte. When expressing the full-length D-endoB FL or D-endoB ∆SH3 mutant transgenes in oocytes, the blockage of vitellogenesis and the defect in fecundity in D-endoB mutants was restored. By contrast, a truncated N-BAR domain of the D-EndoB only partially rescued these defects. Taken together, these results allow us to conclude that D-EndoB contributes to the endocytic activity downstream of Oskar by facilitating membrane dynamics through its N-BAR domain in the yolk uptake process, thereby leading to normal progression of vitellogenesis.

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Section: Introductionmentioning

confidence: 99%

“…The availability of various GFP-tagged proteins means that many cellular processes can be monitored in living cells over the course of minutes or hours, and using this technique, processes such as RNP transport, epithelial morphogenesis, and tissue remodeling have been described in great detail in Drosophila oocytes 1,2 .The ability to perform video imaging combined with a rich repertoire of mutants allows an enormous variety of genes and processes to be examined in incredible detail. One such example is the process of ooplasmic streaming, which initiates at mid-oogenesis 3,4 .…”

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confidence: 99%

Live Imaging of GFP-labeled Proteins in <em>Drosophila</em> Oocytes

Pokrywka

2013

JoVE

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The Drosophila oocyte has been established as a versatile system for investigating fundamental questions such as cytoskeletal function, cell organization, and organelle structure and function. The availability of various GFP-tagged proteins means that many cellular processes can be monitored in living cells over the course of minutes or hours, and using this technique, processes such as RNP transport, epithelial morphogenesis, and tissue remodeling have been described in great detail in Drosophila oocytes 1,2 .The ability to perform video imaging combined with a rich repertoire of mutants allows an enormous variety of genes and processes to be examined in incredible detail. One such example is the process of ooplasmic streaming, which initiates at mid-oogenesis 3,4 . This vigorous movement of cytoplasmic vesicles is microtubule and kinesin-dependent 5 and provides a useful system for investigating cytoskeleton function at these stages.Here I present a protocol for time lapse imaging of living oocytes using virtually any confocal microscopy setup.

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Shining light on Drosophila oogenesis: live imaging of egg development

Cited by 53 publications

References 69 publications

Evolutionarily conserved Wolbachia -encoded factors control pattern of stem-cell niche tropism in Drosophila ovaries and favor infection

Evolutionarily conserved Wolbachia -encoded factors control pattern of stem-cell niche tropism in Drosophila ovaries and favor infection

Endophilin B is required for the Drosophila oocyte to endocytose yolk downstream of Oskar

Live Imaging of GFP-labeled Proteins in <em>Drosophila</em> Oocytes

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