Shoot organogenesis and somatic embryogenesis from leaf and shoot explants of Ochna integerrima (Lour)

Ma, Guohua; Lü, Jinfeng; Silva, Jaime A. Teixeira da; Zhang, Xinhua; Zhao, Jietang

doi:10.1007/s11240-010-9812-7

Cited by 47 publications

(30 citation statements)

References 30 publications

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“…With respect to the culture media, we used the growth regulator TDZ (at 0.9 μM) to induce the embryogenesis in two media that differed in their salt and vitamin composition. This growth regulator was used in the present work since it may increase direct organogenesis and embryogenesis (Gisbert et al, 2006;Chhabra et al, 2008;Ma et al, 2011). Our results show that the salts and vitamins also influenced callus formation, because a higher percentage of direct embryogenesis was obtained in EIM2 compared to EIM1.…”

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confidence: 67%

Mature seeds for in vitro sanitation of the Grapevine leafroll associated virus (GLRaV-1 and GLRaV-3) from grape (Vitis vinifera L.)

Peiró

Gammoudi

Yuste

et al. 2015

Span. j. agric. res.

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<p>The conservation of old grapevine varieties is important since they are adapted to specific climate conditions and may carry genes interesting to breeders. As virus infection is common in grapevine varieties, the use of virus free materials is of great importance. In this work, we used somatic embryogenesis for the sanitation of GLRaV-1 and GLRaV-3 viruses that were found after analyzing the putative presence of the five most common, economically important grape viruses by real-time multiplex RT-PCR in the old cultivar “Grumet Negre”. Unopened and opened inflorescences, fecundated ovaries, and, also, mature seeds were used as starting explants. Explants were<em> </em>cultured on plates with two embryogenesis induction media (Nitsch & McCown Woody plant medium) that contained the growth regulator thidiazuron and differed in their salt and vitamin compositions. One half of each kind of explant was cut prior to being cultured. After five months of culture, embryos had only developed from seeds that were cut previous to sowing. To the best of our knowledge, this is the first time that mature seeds have been used for inducing embryogenesis in grape. A total of 42% of the embryos transferred to tubes for germination regenerated into normal plantlets. The absence of both the GLRaV-1 and GLRaV-3 viruses in all regenerated plants was confirmed by real-time uniplex RT-PCR. So, this protocol can be used for sanitation and also for micropropagation.</p>

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mentioning

confidence: 67%

Mature seeds for in vitro sanitation of the Grapevine leafroll associated virus (GLRaV-1 and GLRaV-3) from grape (Vitis vinifera L.)

Peiró

Gammoudi

Yuste

et al. 2015

Span. j. agric. res.

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“…Error bars indicate 95 % CI multiple shoot regeneration. Similarly, Ma et al (2011) indicated that TDZ has a stronger effect on callus induction and shoot organogenesis than BAP in Ochna integerrima. TDZ can act as a substitute for both the auxin and cytokinin requirements for in vitro morphogenesis (organogenesis and somatic embryogenesis) in several species (Murthy et al 1998).…”

Section: Discussionmentioning

confidence: 94%

Male adventitious roots of Rumex thyrsiflorus Fingerh. as a source of genetically stable micropropagated plantlets

Ślesak

Góralski

Kwolek

et al. 2015

Plant Cell Tiss Organ Cult

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Rumex thyrsiflorus Fingerh. is one of the few dioecious plant species that have sex chromosomes. The chromosome constitution of females is 2n = 12A ? XX and 2n = 12A ? XY 1 Y 2 of males. It is a medicinally important plant species and has also been the object of studies on the structure and function of sex chromosomes and sex ratio. An efficient plant regeneration protocol was developed from karyologically stable male roots that had been derived from a long-term liquid culture. The root segments were grown on MS medium supplemented with the following plant growth regulators: 2.4-D, NAA, kinetin, BAP and TDZ. The highest frequency (81.73 %) of adventitious shoot formation (16.27 shoots/explant) was obtained on MS ? 0.5 mg/l TDZ. Regenerated shoots were successfully rooted on MS ? 2 % sucrose ? 0.5 mg/l IBA and acclimated to in vivo conditions. Histological analysis revealed indirect (via callus) adventitious shoot formation. The cells of the morphogenetic callus were surrounded by a fibrillar structure that was similar to the extracellular matrix. Molecular analysis based on genetic sex markers confirmed that all of the root explants were male. The genetic stability of the regenerated plantlets was confirmed using random amplified polymorphic DNA analysis. This is the first report concerning the micropropagation protocol for R. thyrsiflorus Fingerh. from male roots derived from a long-term liquid culture, which offers a unique opportunity to obtain true-to-type plants of the same sex.

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“…Spontaneous adventitious root formation has been reported in Tuberaria major and Dierama erectum (Goncalves et al 2010;Kothe et al 2010). Difficulties with root induction have also been observed in B. sinuspersici in vitro (Rosnow et al 2011) and in in vitro shoots of Ochna integerrima and Pityopsis ruthii (Ma et al 2011;Wadl et al 2011). The poor rooting ability of these studies may be attributed to the accumulation of metabolic products of BA such as BA9G (Werbrouck et al 1995(Werbrouck et al , 1996Bairu et al 2008).…”

Section: Discussionmentioning

confidence: 99%

Multiple shoot induction and plant regeneration of the single-cell C4 species Bienertia sinuspersici

Northmore

Zhou

Chuong

2011

Plant Cell Tiss Organ Cult

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Direct organogenesis provides an efficient means of propagating plants in vitro without the necessity of growing callus cultures. Shoot regeneration was achieved from bud explants of Bienertia sinuspersici on Murashige and Skoog's media supplemented with 6-benzyladenine (BA). The frequency of regeneration varied based on the concentration of BA used. The highest rate of new shoot induction was observed when explants were cultured on media containing 4.4 lM BA after three weeks. Shoots were successfully elongated on media containing gibberellic acid with the highest shoot elongation observed at 2.9 lM. Elongated shoots developed adventitious roots in media without plant growth regulator and were subsequently transplanted to soil. Light microscopic analysis of cross sections of leaves from regenerated shoots showed similar intracellular cytoplasmic compartmentalization of organelles in chlorenchyma cells compared to those observed in leaves grown under greenhouse conditions. Moreover, Western blot analysis detected high expression of key C 4 photosynthetic enzymes, suggesting the C 4 cycle is maintained in the single cell system. We have developed a procedure for whole plant regeneration via direct organogenesis in B. sinuspersici.

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Shoot organogenesis and somatic embryogenesis from leaf and shoot explants of Ochna integerrima (Lour)

Cited by 47 publications

References 30 publications

Mature seeds for in vitro sanitation of the Grapevine leafroll associated virus (GLRaV-1 and GLRaV-3) from grape (Vitis vinifera L.)

Mature seeds for in vitro sanitation of the Grapevine leafroll associated virus (GLRaV-1 and GLRaV-3) from grape (Vitis vinifera L.)

Male adventitious roots of Rumex thyrsiflorus Fingerh. as a source of genetically stable micropropagated plantlets

Multiple shoot induction and plant regeneration of the single-cell C4 species Bienertia sinuspersici

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