Shoot Regeneration from Leaf and Shoot Derived Callus of Hybrid Poplar(&lt;i&gt;Populus alba&lt;/i&gt; × &lt;i&gt;P. glandulosa&lt;/i&gt;) Clone

Choi, Eunji; Kim, Hak Gon; Yong, Seong-Hyeon; Seol, Yuwon; Park, Dong-Jin; Choi, Myung-Suk

doi:10.14397/jals.2020.54.2.45

“…On the other hand, a successful genetic transformation has been reported using the aspen hybrid poplar "shanxin" from Northern China which is a cross between P. davidiana and P. bollena (Wang et al, 2011;Han et al, 2013;Ding et al, 2017;Han and Han, 2017). In our laboratory, we developed a shoot regeneration technology from the leaf and shoot derived calli of the hybrid poplar (Populus alba × P. glandulosa) (Choi et al, 2020) for the successful generation of transgenic plants using Agrobacterium-mediated transformation (Chung et al, 1989;Choi et al, 2005;Song et al, 2019). Using hybrid poplar, transgenic plants have been successfully generated with several different genes involved in a variety of physiological responses (Zhang et al, 2011;Shim et al, 2013;Choi et al, 2017;Yoon et al, 2018;Kim et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

Drought Stress-Mediated Transcriptome Profile Reveals NCED as a Key Player Modulating Drought Tolerance in Populus davidiana

Lee

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Mun

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Bae

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et al. 2021

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Populus trichocarpa has been studied as a model poplar species through biomolecular approaches and was the first tree species to be genome sequenced. In this study, we employed a high throughput RNA-sequencing (RNA-seq) mediated leaf transcriptome analysis to investigate the response of four different Populus davidiana cultivars to drought stress. Following the RNA-seq, we compared the transcriptome profiles and identified two differentially expressed genes (DEGs) with contrasting expression patterns in the drought-sensitive and tolerant groups, i.e., upregulated in the drought-tolerant P. davidiana groups but downregulated in the sensitive group. Both these genes encode a 9-cis-epoxycarotenoid dioxygenase (NCED), a key enzyme required for abscisic acid (ABA) biosynthesis. The high-performance liquid chromatography (HPLC) measurements showed a significantly higher ABA accumulation in the cultivars of the drought-tolerant group following dehydration. The Arabidopsis nced3 loss-of-function mutants showed a significantly higher sensitivity to drought stress, ~90% of these plants died after 9 days of drought stress treatment. The real-time PCR analysis of several key genes indicated a strict regulation of drought stress at the transcriptional level in the P. davidiana drought-tolerant cultivars. The transgenic P. davidiana NCED3 overexpressing (OE) plants were significantly more tolerant to drought stress as compared with the NCED knock-down RNA interference (RNAi) lines. Further, the NCED OE plants accumulated a significantly higher quantity of ABA and exhibited strict regulation of drought stress at the transcriptional level. Furthermore, we identified several key differences in the amino acid sequence, predicted structure, and co-factor/ligand binding activity of NCED3 between drought-tolerant and susceptible P. davidiana cultivars. Here, we presented the first evidence of the significant role of NCED genes in regulating ABA-dependent drought stress responses in the forest tree P. davidiana and uncovered the molecular basis of NCED3 evolution associated with increased drought tolerance.

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“…On the other hand, a successful genetic transformation has been reported using the aspen hybrid poplar "shanxin" from Northern China which is a cross between P. davidiana and P. bollena (Wang et al, 2011;Han et al, 2013;Ding et al, 2017;Han and Han, 2017). In our laboratory, we developed a shoot regeneration technology from the leaf and shoot derived calli of the hybrid poplar (Populus alba × P. glandulosa) (Choi et al, 2020) for the successful generation of transgenic plants using Agrobacterium-mediated transformation (Chung et al, 1989;Choi et al, 2005;Song et al, 2019). Using hybrid poplar, transgenic plants have been successfully generated with several different genes involved in a variety of physiological responses (Zhang et al, 2011;Shim et al, 2013;Choi et al, 2017;Yoon et al, 2018;Kim et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

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“…On the other hand, a successful genetic transformation has been reported using the aspen hybrid poplar "shanxin" from Northern China which is a cross between P. davidiana and P. bollena (Wang et al, 2011;Han et al, 2013;Ding et al, 2017;Han and Han, 2017). In our laboratory, we developed a shoot regeneration technology from the leaf and shoot derived calli of the hybrid poplar (Populus alba × P. glandulosa) (Choi et al, 2020) for the successful generation of transgenic plants using Agrobacterium-mediated transformation (Chung et al, 1989;Choi et al, 2005;Song et al, 2019). Using hybrid poplar, transgenic plants have been successfully generated with several different genes involved in a variety of physiological responses (Zhang et al, 2011;Shim et al, 2013;Choi et al, 2017;Yoon et al, 2018;Kim et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

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Shoot Regeneration from Leaf and Shoot Derived Callus of Hybrid Poplar(<i>Populus alba</i> × <i>P. glandulosa</i>) Clone

Cited by 6 publications

References 16 publications

Drought Stress-Mediated Transcriptome Profile Reveals NCED as a Key Player Modulating Drought Tolerance in Populus davidiana

Drought Stress-Mediated Transcriptome Profile Reveals NCED as a Key Player Modulating Drought Tolerance in Populus davidiana

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