Essential oils have insecticidal activity against several insects and are composed of numerous compounds. This study investigated the insecticidal effects of the Thuja occidentalis L. (Cupressaceae) essential oil, terpinyl acetate, and bornyl acetate against the peach aphid [Myzus persicae (Sulzer)]. The insecticidal effect of essential oils on three Cupressaceae plants was highest in T. occidentalis. The Thuja occidentalis essential oil was extracted by steam distillation, and the optimum conditions were found. The GC-MS profiling of essential oil components extracted from T. occidentalis leaves identified 16 volatile compounds. The main components were α-thujone (16.58%), β-myrcene (14.62%), bornyl acetate (9.31%), and terpinyl acetate (8.52%). As a result of the metabolite profiling of three Cupressaceae plants, terpinyl acetate and bornyl acetate were present in large amounts in all of the essential oils, and they had an acetate structure, so it was estimated that they had insecticidal activity. The insecticidal activity of these two compounds was stronger than the other individual monoterpene compounds. The addition of surfactants to the terpinyl acetate and bornyl acetate showed strong insecticidal activity. Terpinyl acetate and bornyl acetate can be used as environmentally friendly insecticidal-active compounds.

An efficient plant regeneration protocol for shoot organogenesis from Hovenia dulcis callus cultures was established. Induction of organogenic callus was achieved on Murashige and Skoog (MS) medium supplemented with 4.65 lM kinetin and 4.5 lM 2,4-dichlorophenoxyacetic acid (2,4-D). Further differentiation of organogenic callus into primordia, shoot-like structures, and plantlets was achieved on MS medium supplemented with 0.23 lM gibberellic acid (GA 3 ) and 0.46 lM kinetin. Numerous abnormal shoots developed upon transfer of callus to MS medium containing cytokinins, and these failed to grow further into whole plantlets. However, transfer of 'abnormal' shoots to a fresh MS medium lacking cytokinins resulted in growth of normal shoots. Elongated shoots subsequently were rooted in basal MS medium, and whole plantlets were established in a soil mix. Analysis of regenerated plants using random amplified polymorphic DNA (RAPD) confirmed the genetic stability of these regenerant plantlets.

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Mountain-cultivated ginseng (MCG) is a very important health functional material in Korea and the USA. In this study, the growth and saponin of MCG change according to the sowing method were investigated. Six methods of sowing according to the natural cultivation method that grows in the Sancheong mountain ginseng farm were investigated. Germination rate and budding rate were different according to the sowing method. The growth characteristics of the MCG were also different in the sowing method. Among the six sowing methods, the length of leaves was the widest in BFS. The width of the leaves was wide in DFS and DS. The weight of the shoot was the heaviest in BFS, and the DF was the lightest. Root diameter was highest in BFS and DFS covering both fallen leaves and soil. Total fresh weight was the highest at 4.03 g in BFS, followed by DFS. The S/R ratio of DF was lower than that of the other methods. This suggests that the S/R ratio is lowered because the growth of the ground part is worse. The treatment group with the highest crude saponin content was BF and the lowest treatment group was BS. Shoot and root growth tended to increase with growing period, saponin content increased slightly within 1-3 years, but decreased slightly after that. In particular, the ratio of PD/PT, which has a great influence on the efficacy of ginseng, was also different according to the sowing method. The seed sowing method identified in this study will greatly contribute to the improvement of the survival rate decrease over the cultivation period, and it will also contribute to the production of high-quality MCG.