Shoot Regeneration in Four Begonia Genotypes

Abstract

In vitro regeneration of four Begonia genotypes, B. semperflorens, B. rex, B. × elatior, and hybrid of Begonia with unknown parents 'Tiger' was carried out starting from leaf and petiole segments as explants. Five Murashige and Skoog′s derived media were tested, three of them supplemented with α-naphthaleneacetic acid (NAA) and 6benzyladenine (BA), and the other two with NAA and kinetin (KIN) in different concentrations. Shoot regeneration was preferentially induced on the BA containing media, quantitative differences being observed among explants and genotypes.

In vitro shoot bud differentiation and plantlet regeneration in Celastrus paniculatus Willd

“…BAP appeared to be more effective than Kn for inducing adventitious shoot buds in internode segments of C. paniculatus in agreement with the results described by various authors (Mishra 2002, Espino et al 2004). Kn was effective over BAP on shoot elongation as has also been reported in Rosa hybrida L. (Ibrahim and Debergh 2001).…”

“…(Celastraceae) is a large, woody climbing shrub which occurs naturally in hilly parts of India up to an altitude of 1200 m. The plant is valued for its immense medicinal properties. In vitro adventitious shoot bud regeneration has been achieved from mature explants of several woody trees (Swartz et al 1990, D'Onofrio and Morini 2005, shrubs (Espino et al 2004, Cheepala et al 2004) and herbaceous plant species (Mitra et al 2001, Bacchetta et al 2003. In vitro studies in C. paniculatus has been carried out (Nair and Seeni 2001, Arya et al 2002, Bilochi 2002 but there is no report on its regeneration through adventitious shoot bud differentiation.…”

Micropropagation of an Important Medicinal Plant, Begonia fallax (Begoniaceae)

“…But lower concentration of BA 0.1 mg -l produced only fewer shoots but shoot growth showed enhancement (Simmonds, 1984 (Espino et al, 2004). But, bud break of the nodal segments in B. malabarica was achieved at 4.4 mg -l BA and 1.4 mg -l IAA on MS medicum (Nisha et al, 2009).…”

“…Members of Begoniaceae can easily be distinguished from its allies mostly by fleshy stem, stipulate and usually asymmetrical leaves, showy unisexual flowers with petal-like perianth segments, numerous centripetal stamens, and seeds that uniquely have collar cells below an operculum (Clement et al, 2004).Begonia is one of the ten largest Angiosperm genera and has about 1,630 species, 53 varieties (The Plant List, 2013) and 15,000 cultivars distributed in tropical and subtropical regions (Clement et al, 2004;Espino et al, 2004).This genus is largely divided into 8 groups on the basis of form such as cane-like, rexcultorum, rhizomatous, semperflorens, shrub-like, thickstemmed, trailing or scandent and tuberous (Mendi et al, 2009). Hillebrandia is represented by monotypic, Hillebrandia sandwicensis Oliv.…”

In vitro culture of Bambara groundnut [<i>Vigna subrerranea</i> (L.) Verdc., Favaceae ]: Effect of plant growth regulators, explant type and genotype on callus induction and differentiation

“…According to Kaushal et al (2006), the differential response of explants, from different species to varied hormonal concentrations, can be attributed to the nature of the explant tissue, its genetic potential for regeneration and its nutritional requirement. It was observed that nutritional requirements for optimal growth of a tissue in vitro vary and even tissues from different parts of a plant may have different requirements for satisfactory growth (Espino et al, 2004 ;Zhang et al, 2004). Morphological and biochemical traits of explants affect their cytokinin assimilation and their subsequent ability to initiate calli or shoots (Dhar and Joshi, 2005).…”