Short aggrecan gene repetitive alleles associated with lumbar degenerative disc disease in Turkish patients

Eser, Olcay; Eser, Betül; Coşar, Murat; Mo, Erdogan; Aslan, Adem; Yıldız, Hatice; Solak, Mustafa; Haktanır, Alpay

doi:10.4238/vol10-3gmr1222

Cited by 17 publications

(12 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The findings of this study are similar to those of Eser et al (2011), who investigated 100 Turkish individuals with and without LDH. The most prevalent alleles were A28, A27, and A29.…”

Section: Discussionsupporting

confidence: 89%

“…A 50-µL solution was used, containing 10 pmol sense and antisense sequence, 5 µL genomic DNA, 27.5 mM dNTP (deoxynucleotide triphosphates), 0.3 µL Taq DNA polymerase, 25 mM MgCl 2 , and 10X PCR buffer and water. Amplification was conducted over 38 cycles, with denaturation at 95°C for 5 min, followed by annealing at 66°C for 50 s and extension at 72°C for 50 s. The PCR products were separated on 1.5% agarose gel and visualized using a photodetector (BioRad, USA) after staining with ethidium bromide gel (Eser et al, 2010(Eser et al, , 2011. Alleles were measured using a standard molecular weight of 100 bp.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

CASE-REPORT Association between an ACAN gene variable number tandem repeat polymorphism and lumbar disc herniation: a case control study

et al. 2016

View full text Add to dashboard Cite

ABSTRACT. We investigated the association between an aggrecan gene (ACAN) polymorphism and lumbar disc herniation (LDH). This was a case-control study with quinquennial age and gender groups. The study comprised 119 men and women aged between 20 and 60 from Goiânia (Brazil). Of these, 39 were allocated to the case group (Ca) and 80 to the control group (Ct). We gathered sociodemographic and clinical data, and peripheral blood samples. DNA was isolated for genotyping the ACAN variable number tandem repeat (VNTR) via conventional polymerase chain reaction (PCR). Data were statistically analyzed using the chi-square test, multiple comparison analysis, the Student t-test, and odds ratios, with a level of significance set at 5% (P ≤ 0.05). The groups were homogenous in terms of sociodemographic, anthropometric, and life style variables. The allele score for the ACAN VNTR was significantly lower in volunteers with LDH; the A22 allele was significantly more prevalent in this same group; the Ca group presented greater frequency of short alleles A13-A25, whereas the Ct group presented a higher frequency of long alleles. However, this difference was not statistically significant. In both groups, the most common alleles were A28, A27, and A29, and the A26/A26 genotype was significantly more common in the Ca group. The results showed an association between short alleles and LDH among the investigated adults (Ca), corroborating the hypothesis that aggrecan with shorter repeat lengths can lead to a reduction in the physiological proteoglycan function of intervertebral disc hydration and, consequently, increased individual susceptibility to LDH.

show abstract

“…The findings of this study are similar to those of Eser et al (2011), who investigated 100 Turkish individuals with and without LDH. The most prevalent alleles were A28, A27, and A29.…”

Section: Discussionsupporting

confidence: 89%

Section: Methodsmentioning

confidence: 99%

CASE-REPORT Association between an ACAN gene variable number tandem repeat polymorphism and lumbar disc herniation: a case control study

et al. 2016

View full text Add to dashboard Cite

show abstract

“…Eser et al revealed that short repeated alleles of the aggrecan gene were significantly associated with disc degeneration and multilevel disc degeneration. Their study showed that short repeated alleles of the aggrecan gene are correlated with increased degenerative disc disease in Turkish population (9). A limitation of our study is that we did not analyze the crossover of genes in the patients.…”

Section: █ Discussionmentioning

confidence: 93%

Initial discectomy associated with aging lead to adjacent disc disease and recurrence

Dalgıç

Yıldırım²,

Okay³

et al. 2015

Turkish Neurosurgery

View full text Add to dashboard Cite

difficulties, especially the persistence and recurrence of symptoms. Failure of surgical treatment for LDH can be caused by the true recurrence of disc herniation, new disc herniation at a different disc level, epidural fibrosis, arachnoiditis, foraminal stenosis, and segmental instability (8, 21-23, 29, 30, 32). The overall rate of unsatisfactory discectomy results range from 5% to 20% of recurrent disc herniation and it is the major █ INTRODUCTIONLumbar discectomy is the most common surgical procedure performed by spine surgeons for patients complaining of back and leg pain. Numerous new techniques have been used to improve the efficacy of the surgical excision of herniated intervertebral discs but these procedures still include some AIM: Failure of surgery for lumbar disc herniation (LDH) can be commonly caused by recurrence. There are many debates regarding the risk factors of recurrent LDH (rLDH) and it is very difficult to define them because many clinical and complicated biomechanical parameters are involved. The purpose of study was to evaluate the long term result of re-discectomy for LDH at the same level and adjacent segments. MATERIAL and METHODS:Between 1999 and 2009, 1898 cases were operated and 142 (6.4%) patients underwent re-discectomy following initial operation. The study included 65 patients who were operated for single level discectomy, and their charts were analyzed retrospectively. RESULTS:There were 33 (50.8%) women and mean age was 45.5 years (24-73 years). rLDH was diagnosed at the initial level in 40 (61.5%) but adjacent and/or opposite level herniation (with or without the first level) was found in the remaining 25 cases (39.1%). Recurrence at the same level (SLG) and adjacent level groups (ALG) were similar according to the clinical outcomes in follow-up (mean 34.1 months). Admission period after initial operation was also parallel in SLG and ALG (54.7 and 53.1 months, respectively). However, the mean age of ALG (49.4 years) was significantly higher (p≤0.05) than SLG (42.8 years). CONCLUSION:After discectomy, collapsed discs are biomechanically more stable than those with preserved disc heights, and responses to axial compression on intervertebral disc pressure produced deformations of adjacent levels despite limitations.Altered biomechanical loading next to a fusion resulted in ongoing degeneration with aging at the affected entire lumbar spine.

show abstract

“…Environmental and genetic factors have been reported to play a major role in the pathogenesis of LDH. Previous studies have reported a significant correlation between LDH and polymorphisms in genes coding for aggrecan, vitamin D receptor, collagen, and MMPs (Takahashi et al, 2001;Ala-Kokko, 2002;Eser et al, 2010Eser et al, , 2011.…”

Section: Discussionmentioning

confidence: 95%

Effects of MMP-1 and MMP-3 gene polymorphisms on gene expression and protein level in lumbar disc herniation

Eser¹,

Eser²,

Yüksel³

et al. 2016

Genet. Mol. Res.

Self Cite

View full text Add to dashboard Cite

ABSTRACT. The aim of this study was to identify the possible correlation between polymorphisms in matrix metalloproteinase (MMP)-1 and MMP-3 and their corresponding protein levels in disc 2 B. Eser et al. Genetics and Molecular Research 15 (3): gmr.15038669 tissues obtained from patients with lumbar disc herniation (LDH) using biochemical and immunohistochemical analyses. Blood and disc samples were obtained from 100 patients with LDH who underwent a lumbar microdiscectomy. Based on the radiological degeneration, the patients were diagnosed with grade 2, 3, or 4 LDH. MMP-1 -1607 1G/2G and MMP-3 -1171 5A/6A were analyzed by real-time polymerase chain reaction. The expressions of MMP-1 and MMP-3 were detected by biochemical and immunohistochemical analyses. We found no association between the MMP-1 polymorphism and disc degeneration and MMP-1 expression. However, patients expressing the 6A/6A and 5A/6A alleles of MMP-3 -11715A/6A showed higher MMP-3 expression, compared to those expressing the 5A/5A genotype. Additionally, the radiological degeneration grades were correlated with the histological degeneration scoring. Protein levels and immunopositive cell rates of MMP-1 and MMP-3 were associated with disc degeneration grades. Moreover, the MMP-1 and MMP-3 expression and the histological and radiological scores were positively correlated and the MMP-3 -11715A/6A polymorphism was associated with MMP-3 expression in herniated disc tissues. This study is the first to investigate polymorphisms in MMP-1 and MMP-3, as well as their corresponding protein expressions. We also quantified an association between the radiological degeneration grades and MMP-1 and MMP-3 expression. Further genomic studies on MMPs could focus on the utilization of MMP-1 and MMP-3 as markers for the prevention and treatment of this disease.

show abstract

Short aggrecan gene repetitive alleles associated with lumbar degenerative disc disease in Turkish patients

Cited by 17 publications

References 24 publications

CASE-REPORT Association between an ACAN gene variable number tandem repeat polymorphism and lumbar disc herniation: a case control study

CASE-REPORT Association between an ACAN gene variable number tandem repeat polymorphism and lumbar disc herniation: a case control study

Initial discectomy associated with aging lead to adjacent disc disease and recurrence

Effects of MMP-1 and MMP-3 gene polymorphisms on gene expression and protein level in lumbar disc herniation

Contact Info

Product

Resources

About