Short Communication Differential expression analysis of porcine MDH1, MDH2 and ME1 genes in adipose tissues

Zhou, Shuling; Li, M Z; Li, Q H; Guan, Jiuqiang; Li, X W

doi:10.4238/2012.may.9.4

Cited by 26 publications

(20 citation statements)

References 14 publications

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“…Other metabolically important genes -ACACA and SREBF1also displayed comparably higher transcript levels in subcutaneous tissue, and the PL pigs showed an increased ACACA transcript level in the subcutaneous tissue and lower in the longissimus muscle when compared to the other breeds (PLW, Duroc and Pietrain) (Stachowiak et al 2013). Our results also confirm the data reported by Zhou et al (2012) who observed an increased ME1 transcript level in the adipose tissue of a local Chinese fatty pig breed (Rongchang) when compared with the leaner Landrace pigs. It must be noted, however, that it was not the PL breed but rather the Pietrain gilts (N = 12), which were phenotypically different from the other breeds with respect to most production traits (average backfat thickness p < 9.0 9 10 À5 , abdominal fat weight p = 3.4 9 10 À7 , lean meat content p = 5.6 9 10 À14 , daily gain p < 6.0 9 10 À4 ) except intramuscular fat content.…”

Section: Me1 Transcript Levelsupporting

confidence: 90%

Transcript level of the porcine ME1 gene is affected by SNP in its 3′UTR, which is also associated with subcutaneous fat thickness

Bartz

Kociucka

Mańkowska

et al. 2013

J Animal Breeding Genetics

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Pork quality depends on multiple factors, including fatty acid composition in muscle and fat tissues. The ME1 gene is a strong candidate for fat accumulation, as it encodes the malic enzyme, which is required for fatty acid synthesis. We identified seven new polymorphisms in 3'UTR of the ME1 gene and moreover confirmed the presence of 4 polymorphisms detected previously. Interestingly, the studied Duroc pigs were monomorphic at all these polymorphic sites, while in 3 other breeds (Pietrain, Polish Landrace and Polish Large White), the polymorphisms were unevenly distributed. One of the novel SNPs (c.*488A>G) was found in the Polish Large White and the Polish Landrace only, and the association studies revealed that it was significantly associated with backfat thickness and average daily weight gain in the Polish Landrace (N = 207) and the Polish Large White (N = 157). This SNP was differently associated with ME1 transcript level in muscle and backfat. The in silico analysis of another novel SNP (c.*548C>T) indicated that it is located within a binding sequence conserved among vertebrates for the miR-30 family in 3'UTR of the ME1. It was shown that in the longissimus muscle, but not in adipose tissue, CT gilts compared with CC ones had significantly lower levels of the ME1 transcript. This polymorphism, however, was not associated with production traits. Additionally, we observed that transcript level of the ME1 was significantly higher in subcutaneous fat than in the longissimus muscle, as well as both investigated tissues of the Polish Landrace when compared to the other breeds. However, no association was found between this polymorphism and fatty acid profiles. We conclude that the ME1 gene polymorphism (c.*488A>G) is a potential marker for porcine backfat thickness.

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Section: Me1 Transcript Levelsupporting

confidence: 90%

Transcript level of the porcine ME1 gene is affected by SNP in its 3′UTR, which is also associated with subcutaneous fat thickness

Bartz

Kociucka

Mańkowska

et al. 2013

J Animal Breeding Genetics

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“…Rongchang pig is a representative obese type of indigenous breed. In previous study, researchers seemed to be more interested in adipose tissue of Rongchang pig such as the expression level of some genes, gene polymorphism and so on (Jiang, Hua, et al, ; Zhou, Li, Li, Guan, & Li, ), while some studies on gene expression profiling of skeletal muscle (Fang, Yin, Li, Zhang, & Watford, ; Zhang, et al, ). However, information available on the expression profiling of miRNAs during myogenesis of Rongchang pig is inconclusive.…”

Section: Discussionmentioning

confidence: 99%

Deciphering the miRNA transcriptome of Rongchang pig longissimus dorsi at weaning and slaughter time points

Chen

Zhao

Dou

et al. 2020

Animal Physiology Nutrition

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MicroRNA (miRNA) is essential for the process of gene posttranscriptional regulation in skeletal muscle of many species, such as mice, cattle and so on. However, a little number of miRNAs have been reported in the muscle development of Chinese native pig breeds. In this study, the longissimus dorsi transcripts of Chinese native Rongchang pig at weaning and slaughter time points were analysed for miRNA‐seq. The results showed that 19 novel and 186 known miRNAs involved in the Rongchang pig skeletal muscle development were identified. Based on these findings, we further confirmed that porcine miR‐127, miR‐299 and miR‐432‐5p were obviously down‐expressed in adult pig (287 days of age), while miR‐7134‐3p and 664‐5p were significantly up‐expressed in weaning pig (35 days of age). In other words, these miRNAs could be the potential molecular markers and play vital roles in the muscle development process. Moreover, we found miR‐127 could inhibit the proliferation and myogenesis of porcine satellite cells in longissimus dorsi muscle. Our findings will provide deep insight into miRNA function for pork quality research with Chinese indigenous pig breeds.

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“…The present results revealed that the cytosolic (Mdh-1 and ME-1) were the only expressed isoenzymes in most samples of racing camel while both cytosolic and mitochondrial isoforms were expressed in production breed. The two essential malate dehydrogenases, Mdh-1 (cytosolic malate dehydrogenase) and Mdh-2 (mitochondrial malate dehydrogenase), play important roles in the Krebs cycle for energy production through aerobic respiration [27]. Mdh-1 and Mdh-2 are also involved in lipid synthesis by providing extramitochondrial reducing equivalents from the oxidation of malate [28,29].…”

Section: Resultsmentioning

confidence: 99%

Comparison of energy-related isoenzymes between production and racing Arabian camels

AL-Harbi¹,

Amer²

2012

ABB

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Native polyacrylamide gel electrophoresis have been applied for the analyses of alkaline phosphatase (ALP), malate dehydrogenase (Mdh) and malic (ME) isoenzymes in Arabian camel for racing and production. Two fractions for each of these isoenzymes have been recorded in the studied breeds. ALP showed very weak patterns without remarkable difference between the two breeds and this is an indication to that the samples used were healthy and being from the same age. The cytosolic Mdh-1 and ME-1 have been recorded in both camel breeds with high intensity. The mitochondrial Mdh-2 and ME-2 have been recorded with small intensity in production breeds commonly. The present data indicate the necessity of the mitochondrialMdh-2 for energy production in racing breed and the responsibility of the cytosolic Mdh-1 for lipogenesis and energy production in both breeds. We therefore may assume that the appearance of both Mdh forms is necessary for both energy and lipid production in the production breeds while Mdh-1 was useful as bioenergetic enzyme necessary for racing. The different expressions are indications of the difference in the physiological adaptations of both camel breeds and are not for a systematic value

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Short Communication Differential expression analysis of porcine MDH1, MDH2 and ME1 genes in adipose tissues

Cited by 26 publications

References 14 publications

Transcript level of the porcine ME1 gene is affected by SNP in its 3′UTR, which is also associated with subcutaneous fat thickness

Transcript level of the porcine ME1 gene is affected by SNP in its 3′UTR, which is also associated with subcutaneous fat thickness

Deciphering the miRNA transcriptome of Rongchang pig longissimus dorsi at weaning and slaughter time points

Comparison of energy-related isoenzymes between production and racing Arabian camels

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