Short Communication: In vitro antimicrobial and antimalarial screening of a crude extract of Streptomyces sp. AB8 isolated from Lapindo Mud Volcano Area, Sidoarjo, Indonesia

Setyaningrum, Endah; Nukmal, Nismah; Aeny, Titik Nur

doi:10.13057/biodiv/d220731

Cited by 4 publications

(2 citation statements)

References 36 publications

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“…AB8 against P. falciparum 3D7 in vitro was 17.56 μg/mL. 46 Another study stated that parasite inhibition of S. hygroscopicus subsp. Hygroscopicus strain i18 towards P. falciparum in vitro as measured using probit analysis was 11.07 μg/mL.…”

Section: Discussionmentioning

confidence: 99%

Fractions 14 and 36K of Metabolite Extract Streptomyces hygroscopicus subsp. Hygroscopicus Have Antimalarial Activities Against Plasmodium berghei in vitro

Fitri¹,

Endharti²,

Abidah³

et al. 2023

IDR

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Purpose The study was conducted to investigate the effectivity and the cytotoxicity of fractions 14 and 36K of metabolite extract of Streptomyces hygroscopicus subsp. Hygroscopicus as an antimalarial compounds against Plasmodium berghei in vitro. Methods Fractions 14 and 36K of metabolite extract of Streptomyces hygroscopicus subsp. Hygroscopicus produced by the fractionation process utilizing the Flash Column Chromatography (FCC) BUCHI Reveleris ® PREP. Plasmodium berghei culture was used to assess the antimalarial activity of fractions 14 and 36K. Parasite densities and the ability of parasite growth were determined under microscopic. The cytotoxicity of the fractions was assessed using MTT assays on the MCF-7 cell line. Results Streptomyces hygroscopicus subsp. Hygroscopicus fractions 14 and 36K have antimalarial activity against Plasmodium berghei , with fraction 14 having the more potent activity. The percentage of Plasmodium berghei -infected erythrocytes was decreased as well as the increase of fraction concentration. Fraction 14 has the highest inhibition of parasite growth at a concentration of 156,25 μg/mL, with an inhibition percentage of 67.73% (R 2 = 0.953, p = 0.000). IC 50 of fractions 14 and 36K were found at 10.63 μg/mL and 135,91 μg/mL, respectively. The fractions caused morphological damage in almost all asexual stages of the parasite. Both fractions were not toxic against MCF-7, indicating that the fractions have a safe active metabolite. Conclusion Fractions 14 and 36K of metabolite extract Streptomyces hygroscopicus subsp. Hygroscopicus contains non-toxic compounds that could damage the morphology and inhibit the growth of Plasmodium berghei in vitro.

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Section: Discussionmentioning

confidence: 99%

Fractions 14 and 36K of Metabolite Extract Streptomyces hygroscopicus subsp. Hygroscopicus Have Antimalarial Activities Against Plasmodium berghei in vitro

Fitri¹,

Endharti²,

Abidah³

et al. 2023

IDR

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“…Furthermore, these natural chemicals protect cells against DNA damage caused by different carcinogens, including ROS, owing to their antioxidant properties. 27 Thus, they reduce DNA damage and prevent genetic instability that leads to β cell destruction. 28 Consequently, we observed a recovery of damaged β cells to their normal conditions after treatment with the ethanol extract of cherry leaves at 300, 400, and 500 mg/kg bw.…”

Section: Discussionmentioning

confidence: 99%

Kersen (Muntingia calabura L.) Ethanol Extract Repairs Pancreatic Cell Damage, Total Coliforms, and Lactic Acid Bacteria in Hyperglycemic Mice

Busman¹,

Lestari²,

Rosa³

2022

J. Pure Appl. Microbiol.

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Hyperglycemia is a condition in which the blood glucose levels exceed normal limits. Ethanol extract of Jamaican cherry leaves (Muntingia calabura L.) contains active components that have the potential to lower blood sugar levels and heal pancreatic cell damage in rats. Gut microbiota imbalance can cause immune system abnormalities, illnesses, and metabolic disorders, including diabetes. The aim of the present study was to determine whether an ethanol extract of Jamaican cherry leaves can repair pancreatic cell damage, as well as influence the total coliforms and lactic acid bacteria in hyperglycemic mice. In this study, 25 mice were randomly placed into five groups, which were then provided with food and drinking water. Alloxan in a dose of 160 mg/kg bw was administered to the positive control group and three treatment groups. Each of the three groups was then treated with the ethanol extract of Jamaican cherry leaves at doses of 300, 400, or 500 mg/kg bw for 14 days. The groups that received the extract were able to repair the pancreatic damage considerably by increasing the number of normal pancreatic cells. This was supported by histological observations. The total abundance of lactic acid bacteria in hyperglycemic mice was 1.08 ± 45 x 1010 CFU/g, which was substantially lower than that in mice treated with the extract. The overall coliform abundance in hyperglycemic mice was 4.37 ± 41 x 1010 CFU/g, which was higher than that in mice supplemented with the extract. Therefore, the administration of Jamaican cherry leaf ethanol extract on a regular basis can increase the abundance of cecum microbiota in hyperglycemic mice. Furthermore, this extract can be used as a natural alternative treatment for recovering pancreatic cell damage.

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Improving the glass ionomer restoration by Incorporating strontium NPs coated with AuNPs manufactured by laser ablation in liquid

Jaber,

Khashan,

Jamal Abbas

et al. 2024

Inorganic Chemistry Communications

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Short Communication: In vitro antimicrobial and antimalarial screening of a crude extract of Streptomyces sp. AB8 isolated from Lapindo Mud Volcano Area, Sidoarjo, Indonesia

Cited by 4 publications

References 36 publications

Fractions 14 and 36K of Metabolite Extract Streptomyces hygroscopicus subsp. Hygroscopicus Have Antimalarial Activities Against Plasmodium berghei in vitro

Fractions 14 and 36K of Metabolite Extract Streptomyces hygroscopicus subsp. Hygroscopicus Have Antimalarial Activities Against Plasmodium berghei in vitro

Kersen (Muntingia calabura L.) Ethanol Extract Repairs Pancreatic Cell Damage, Total Coliforms, and Lactic Acid Bacteria in Hyperglycemic Mice

Improving the glass ionomer restoration by Incorporating strontium NPs coated with AuNPs manufactured by laser ablation in liquid

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