Short Communication Isolation and characterization of eight polymorphic microsatellite loci for the coconut pest, Brontispa longissima (Coleoptera: Hispidae)

Abstract

ABSTRACT. Brontispa longissima is one of the most serious insect pests of coconut in Southeast Asia; it was first discovered on Hainan Island in June 2002. Despite the economic risk associated with this pest, genetic aspects of the invasion process have remained relatively unexplored. Using microsatellite markers, we investigated the population structure, genetic variability and pattern of invasion in various geographic populations. The methodology was based on a modified biotin-capture method. Eight polymorphic microsatellite loci were isolated and characterized for the pest. The allele number per locus varied from 2 to 3 (N = 30). The expected and observed heterozygosities of the eight loci ranged from 0.042 to 0.509 and from 0.042 to 0.963, respectively. Although the frequency of polymorphisms was not very high in this population, the microsatellite loci that were isolated will be useful for investigating the genetic

Microsatellite Analysis Reveals Population Structure and Population Expansion ofTecia solanivora1inSolanum tuberosumin Colombia

“…Microsatellites provide insight into the effect of gene flow on the genetic structure of populations (Freeland 2005) with implications for evolutionary processes underlying population genetics, conservation biology, and pest management (Peccoud et al 2008, Ross and Shoemaker 2008, Ma et al 2011. They provide information on genetic variation within and between individuals of a population based on the numbers of alleles per individual, alleles fixed in a population, and alleles shared between populations (Freeland 2005).…”

“…They provide information on genetic variation within and between individuals of a population based on the numbers of alleles per individual, alleles fixed in a population, and alleles shared between populations (Freeland 2005). The markers have been used for several species of insect pests including Bemisia tabaci (whitefly) (Gennadius 1889) (Hemiptera: Aleyrodidae) from the Asia-Pacific region where De Barro et al (2005) found six distinct populations because of no gene flow among them; invasive behavior of Brontispa longissima (coconut hispine beetle) (Gestro 1885) (Coleoptera: Hispidae), the most serious pest of Cocos nucifera (Linn) (Arecaceae: Arecales) in Southeast Asia (Ma et al 2011); to estimate the number of founders in the United States of Solenopsis invicta (fire ant) (Buren 1972) (Hymenoptera: Formicidae) the World Conservation Union considers one of the 100 worst invasive alien species (Ross and Shoemaker 2008); to determine lack of genetic differentiation among populations of the moth Plutella xyllostella (diamondback moth) (Linnaeus 1758) (Lepidoptera: Yponomeutoidea) in Australia (Endersby et al 2006); and determine little genetic differentiation in the moth Cydia pomonella (L.) (Lepidoptera: Tortricidae) in Chile (Fuentes-Contreras et al 2008). Torres-Leguizamón et al (2009) isolated and characterized nine microsatellites from Tecia solanivora (Povolny) (Lepidoptera: Gelechiidae) in Central America (Costa Rica and Guatemala).…”

Development of Single Nucleotide Polymorphism (SNP) Markers for Analysis of Population Structure and Invasion Pathway in the Coconut Leaf Beetle Brontispa longissima (Gestro) Using Restriction Site-Associated DNA (RAD) Genotyping in Southern China

“…One named Pacific clade is distributed in a relatively limited area (Papua New Guinea, Australia, Samoa, and Sumba Island), whereas the other named Asian clade covers a wide area, including Asia (i.e., Indonesia, Cambodia, Japan, Myanmar, the Philippines, Taiwan, Thailand, and Vietnam) and the French Polynesia, New Caledonia, and Vanuatu [2]. Since the late 1930s, it has invaded Pacific islands [3,4]. In 1975, the beetle was introduced to Taiwan [5], and then spread to Hong Kong, Hainan, Guangdong, Guangxi, Yunnan, and Fujian provinces in China [6].…”

Keragaman Fenotipe dan Genetik Kumbang Brontispa longissima (Coleoptera: Chrysomelidae) pada Tanaman Kelapa; The Genetic and Phenotypic Diversities of Brontispa longissima Beetle (Coleoptera: Chrysomelidae) on Coconut Palms

“…Pada sebelas tahun terakhir, B. longissima telah menyebar dengan cepat dan menimbulkan kerusakan berat di kawasan Asia (Cambodia, Indonesia, Jepang, Myanmar, Filipina, Taiwan, Thailand dan Vietnam) (Takano et al, 2011;Yamashita et al, 2008), Australia (Darwin, Broome, kepulauan Moa, Cooktown, Cairns, Innisfail, Marcoola dan Townsville) (Ma et al, 2011) dan Pacific (French, Polynesia, New Caledonia dan Vanuatu) (Takano et al, 2011;Takano et al, 2013), sehingga merubah status hama ini menjadi hama penting (Rethinam & Singh, 2007). B. longissima telah dimasukkan sebagai hama invasif (Anonim, 2009).…”