Short Communication Prevalence of Trypanosoma evansi in Water Buffaloes in Remote Areas in Northern Vietnam Using PCR and Serological Methods

Holland, Wicher; Thanh, Ng; My, Ln; Do, Tt; Goddeeris, Bruno; Vercruysse, Jozef

doi:10.1023/b:trop.0000009521.13984.17

Cited by 34 publications

(15 citation statements)

References 8 publications

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“…Five provinces were chosen at random from areas with high water buffalo populations that were located at least 100km from each other. (Holland et al, 2004). In total, 484 serum samples were collected during the survey.…”

Section: Field Surveymentioning

confidence: 99%

Diagnostic value of the recombinant tandem repeat antigen TeGM6-4r for surra in water buffaloes

Nguyen

Zhou

Ngasaman

et al. 2014

Veterinary Parasitology

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Trypanosoma evansi infection, or surra, is currently affecting various species of animals, especially water buffaloes. Since diagnosis is an important aspect of surra control, development of novel diagnostic antigens is of interest to implement and improve the currently utilized methods. Our study evaluated the tandem repeat antigen TeGM6-4r in T. evansi antibody detection in water buffaloes. TeGM6-4r-based ELISA was performed with 20 positive and 8 negative controls and 484 field samples from water buffaloes in Northern Vietnam. To examine cross-reactivity, sera from Japanese cattle that had been experimentally infected with Theileria orientalis (n=10), Babesia bovis (n=3), Babesia bigemina (n=7) and Trypanosoma theileri (n=59) were included in the study. The sensitivity of the test was 80%. TeGM6-4r did not react with Theileria or Babesia infected sera, however it showed cross reactivity with 11/59 T. theileri infected samples. The reference test, CATT/T. evansi also reacted with 3/59 T. theileri infected sera. The lysate antigen-based ELISA reacted with 4/59 T. theileri, 9/10 Theileria and 3/10 Babesia infected sera. In contrast, TeGM6-4r-based ELISA was 86.3% sensitive and 58.3% specific in the screening of field samples. The average seroprevalence of T. evansi infection among water buffaloes in Northern Vietnam was 27.1% by CATT/T. evansi and 53.7% by TeGM6-4r. Seroprevalence in the five surveyed provinces ranged from 17.4% to 39.8% in the reference test, and 47.3% to 67.3% in the recombinant antigen based test. The finding indicated that the disease is still widely endemic in the area and that surveillance programs need to be carried out regularly to better control surra. We proposed TeGM6-4r as a useful serodiagnostic antigen for the detection and epidemiological surveillance of T. evansi infection among water buffaloes.

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Section: Field Surveymentioning

confidence: 99%

Diagnostic value of the recombinant tandem repeat antigen TeGM6-4r for surra in water buffaloes

Nguyen

Zhou

Ngasaman

et al. 2014

Veterinary Parasitology

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“…Standardization and validation methods have been developed [ 12 – 15 ]. ELISA T. evansi , initially used on camels [ 16 ], has been adapted to other species such as horses [ 17 , 18 ], buffaloes [ 19 ], cattle [ 20 ], and pigs [ 21 ]. However, the local development of serological tests, including parasite production, mean population responses, and selection of control sera is necessary to cope with the variability in response that may exist between parasite stocks and hosts from different geographic areas [ 13 , 22 ].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of an Indirect-ELISA Test forTrypanosoma evansiInfection (Surra) in Buffaloes and Its Application to a Serological Survey in Thailand

Kocher

Desquesnes

Kamyingkird

et al. 2015

BioMed Research International

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Surra, caused by Trypanosoma evansi, is a neglected disease due to frequent subclinical evolution, especially in bovines in Asia. However, acute and chronic signs are regularly observed, with significant sanitary and economic impacts. In this study, we evaluated and applied an indirect-ELISA test for the detection of anti-T. evansi immunoglobulin G in buffaloes using antibovine conjugate. Based on buffalo reference sera from the Philippines, a two-graph receiver operating characteristics analysis (TG-ROC) was conducted to define an optimal cut-off value; sensitivity and specificity were estimated at 92.5% and 94.2%, respectively. A cross-sectional serological survey was carried out in the major buffalo breeding areas of Thailand; 892 buffaloes from 8 provinces were sampled in North, Northeastern, and Southern Thailand. Seropositive buffaloes were found in all 8 provinces, on 20.3% of farms for an overall prevalence of 12.2% (95% CI 10.2–14.5%). Nearly one-third of the sampled population was exposed to infection. Broader sampling would be necessary but is not possible in the southern half-wild breeding systems. According to our results, buffaloes may constitute a large and robust reservoir for T. evansi, which is a permanent threat to other livestock such as cattle and horses as well as wild animals such as elephants in Southest Asia.

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“…Following on from the surveys of Thu et al [14] and Holland et al [5], this paper reports the current seroprevalence of surra among water buffaloes in the mountainous region of North Vietnam and the effectiveness of 2 available trypanocidal drugs (Trypamidium ® and Berenil ® ). The study area was in the Cao Bang and Thai Nguyen Provinces in Northern Vietnam, which have a high population of water buffaloes and are known to be endemic for surra (Fig.…”

mentioning

confidence: 99%

“…A good understanding on the occurrence of the disease would guide veterinary officers and policy makers to implement a better preventative and treatment program [6]. However, there is a lack of information on the current prevalence and distribution of T. evansi infection in Vietnam, since the last surveys were completed in 1998 and 2000 [5,14,15]. Aside from epidemiological studies, chemotherapy administration is another aspect of disease control.…”

mentioning

confidence: 99%

Seroprevalence of <i>Trypanosoma evansi</i> Infection in Water Buffaloes from the Mountainous Region of North Vietnam and Effectiveness of Trypanocidal Drug Treatment

Nguyen

Pham

et al. 2013

The Journal of Veterinary Medical Science

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ABSTRACT. Water buffalo (WB) is an important domestic animal in Vietnam. This study utilized a card agglutination test to investigate seroprevalence of surra in WB population. Sera were collected from 585 WB from 4 different regions in Cao Bang and Thai Nguyen Provinces. Among them, 131 samples (22.4%) were positive for surra. The highest prevalence (24.6%) was found among 3 to 5 years old WB. Buffaloes less than 3 years old had the lowest prevalence (15.6%). Among 27 abortion cases, 9 WB (33.3%) were surra positive. For treatment of surra, Berenil ® demonstrated a 100% cure rate, while that of Trypamidium ® was only 40%. Our findings suggest that the current control strategy has not succeeded in reducing prevalence of surra in Vietnam.

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Short Communication Prevalence of Trypanosoma evansi in Water Buffaloes in Remote Areas in Northern Vietnam Using PCR and Serological Methods

Cited by 34 publications

References 8 publications

Diagnostic value of the recombinant tandem repeat antigen TeGM6-4r for surra in water buffaloes

Diagnostic value of the recombinant tandem repeat antigen TeGM6-4r for surra in water buffaloes

Evaluation of an Indirect-ELISA Test forTrypanosoma evansiInfection (Surra) in Buffaloes and Its Application to a Serological Survey in Thailand

Seroprevalence of <i>Trypanosoma evansi</i> Infection in Water Buffaloes from the Mountainous Region of North Vietnam and Effectiveness of Trypanocidal Drug Treatment

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