Short communication: Progressive motility of frozen–thawed canine semen is highest five minutes after thawing

Karger, S; Geiser, B; Grau, Manuel; Heuwieser, W.; Arlt, Sebastian

doi:10.1111/rda.12905

Cited by 4 publications

(2 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The present study results are in corroboration with the kinetic parameters reported for jack semen previously (Prashant et al 2019). A significant decrease in total and progressive motility was recorded in all jack stallions, supporting the general agreement that the semen of the donkey was less efficiently frozen and freezing process can contribute to a decline in sperm motility, loss of membrane integrity and acrosome structure and DNA integrity (Karger et al 2017).…”

Section: Resultssupporting

confidence: 91%

Supplementation of caffeine to extender improves post-thaw seminal attributes of Poitou donkey semen

Kant

Pal

Legha

et al. 2021

Indian J of Anim Sci

View full text Add to dashboard Cite

The current study was conducted with an objective of elucidating the effect of addition of various levels of caffeine to jack semen on seminal attributes at pre- and post-thaw stage. Six ejaculates from each three adult jack stallions were collected via artificial vagina using estrus jenny as dummy. Caffeine was added to semen extender at 0.1, 0.5 and 2 mM at pre-freeze stage and the semen was cryopreserved according to standard protocol. Sperm motility, viability and other functional attributes were assessed at pre-freeze and post-thaw stages. The present study revealed that, adding caffeine to jack semen extender at 0.1 mM, 0.5 mM or 2 mM levels had positive impact on seminal parameters compared to the control semen. Sperm kinetics (VAP, VSL and STR) and motility increased in the caffeine treated groups in comparison to the control group. The caffeine at 2.0 mM concentration resulted in better post-thaw sperm motility, viability, and acrosome integrity as compared to 1 or 0.5 mM of caffeine and control. Caffeine supplementation also enhanced post-thaw sperm kinematic parameters, which implies its potential as an alternative antioxidant supplement. It was concluded that, 2 mM caffeine supplementation to donkey semen significantly improved post-thaw sperm motility and other functional attributes as compared to the control. However, the potential benefits of caffeine on the fertility rates needs to be investigated.

show abstract

Section: Resultssupporting

confidence: 91%

Supplementation of caffeine to extender improves post-thaw seminal attributes of Poitou donkey semen

Kant

Pal

Legha

et al. 2021

Indian J of Anim Sci

View full text Add to dashboard Cite

show abstract

“…Sperm DNA damage, loss of membrane integrity, and changes to the acrosome's structure can all be brought on by cryopreservation (Karger et al, 2017;Kim et al, 2010) The aforementioned dangers call for novel approaches to improve canine spermatozoa post-thaw parameters, maximize oocyte fertilization, and boost contraceptive rates.…”

Section: Introductionmentioning

confidence: 99%

Effect of tris-citric acid-egg yolk-glycerol and vitrified medium with and without ascorbic acid on the quality of post- thaw canine semen

Elbehiry

Abdelkéfi²,

Hattab³

et al. 2023

Damanhour Journal of Veterinary Sciences

View full text Add to dashboard Cite

The study aimed to compare vitrified medium (Soya lecithin 1% and 0.25 M sucrose) with tris-citric acid fructose-egg yolk 20%-glycerol 3% (TCF-EY-GL) for freezing of canine semen and assess the impact of adding ascorbic acid to both extenders on the post-thawing semen quality. Semen was collected from 3 healthy and fertile German shepherd dogs by digital manipulation and stimulation of the bulbus glandis. Collected semen was initially evaluated by a computerized assisted semen analyzer (CASA). Ejaculates from the dogs were pooled and divided into 4 equal parts and diluted 1:1 with the following 4 extenders: TCF-EY-GL with ascorbic acid (Ex1), TCF-EY-GL without ascorbic acid (Ex2), vitrified medium with ascorbic acid (Ex3) and vitrified medium without ascorbic acid (Ex4). The extended semen allowed for equilibrating for 1 h in a refrigerator set at 4 ºC then packed into 0.5ml straws. Straws containing semen diluted in Ex1 and2 were frozen by the conventional method however, those containing semen diluted in Ex3 and Ex4 (vitrified media) were plunged directly in liquid nitrogen. Semen samples were evaluated by CASA immediately after thawing. Results revealed that progressive motility, normal morphology, and acrosome integrity were significantly higher in vitrified medium (Soya lecithin 1% and 0.25 M sucrose) than those obtained in TCF-EY-glycerol however, plasma membrane integrity was significantly improved in TCF-EY-GL compared with that observed in the vitrified medium. Addition of ascorbic acids to vitrified medium and TCF-EY-GL resulted in a significant increase in all semen quality parameters. Total sperm motility, progressive motility, and normal sperm morphology were significantly higher in vitrified medium with ascorbic acid than those observed in TCF-EY-GL with ascorbic acid. It was concluded that the addition of ascorbic acid to vitrified medium or TCF-EY-GL extender resulted in significant improvement in postthawing canine semen quality and the vitrified medium can be used as a good alternative for TCF-EY-GL extender to facilitate freezing and avoid undesirable use of EY.

show abstract

Assessment of Optimized Frozen/Thawed Semen Samples in Canines with the New A-Kinase Anchor Protein 4 Precursor Biomarker

Langlade

Buff

Dias

et al. 2020

Biopreservation and Biobanking

View full text Add to dashboard Cite

Short communication: Progressive motility of frozen–thawed canine semen is highest five minutes after thawing

Cited by 4 publications

References 13 publications

Supplementation of caffeine to extender improves post-thaw seminal attributes of Poitou donkey semen

Supplementation of caffeine to extender improves post-thaw seminal attributes of Poitou donkey semen

Effect of tris-citric acid-egg yolk-glycerol and vitrified medium with and without ascorbic acid on the quality of post- thaw canine semen

Assessment of Optimized Frozen/Thawed Semen Samples in Canines with the New A-Kinase Anchor Protein 4 Precursor Biomarker

Contact Info

Product

Resources

About