Short Communication:  Single nucleotide polymorphism in C-type lysozyme gene and its correlation with Aeromonas hydrophila resistance in African catfish Clarias gariepinus

Nasrullah, Hasan; Nababan, Yanti Inneke; Safitri, Ika; Yanti, Dwi; Nuryati, Sri; Zairin, Muhammad; Ali, Alimuddin

doi:10.13057/biodiv/d210138

Cited by 3 publications

(1 citation statement)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…One of the diseases that may infect freshwater fish is Motile Aeromonas Septicemia (MAS), caused by Aeromonas hydrophila bacteria (Nasrullah et al 2020). Bacterial infection by A. hydrophila causes 80% of the deaths of cultured fish at various stages.…”

Section: Introductionmentioning

confidence: 99%

Potential extracts of wedge sea hare (Dolabella auricularia) as immunostimulators in comet fish (Carassius auratus auratus) infected by Aeromonas hydrophila

2022

View full text Add to dashboard Cite

Abstract. Nurhalisa N, Nur I, Suryani S. 2022. Potential extracts of wedge sea hare (Dolabella auricularia) as immunostimulators in comet fish (Carassius auratus auratus) infected by Aeromonas hydrophila. Biodiversitas 23: 1884-1893. Microencapsulation is an innovative method to protect the active compound of pharmaceutical material from environmental impacts or unwanted conditions. This study aims to determine the active compound content and appropriate concentration of wedge sea hare extract (Dolabella auricularia) using the microencapsulation method to treat Aeromonas hydrophila bacterial which infected in comet fish (Carassius auratus auratus). The treatment consisted of three concentrations of D. auricularia extract (6.3, 7 and 7.7 g/kg feed) and commercial feed as a positive control in triplicates. C. a. auratus fed with experimental diets twice a day (8.00 a.m and 4.00 p.m) for 30 days of culture period before being challenged with an injection of A. hydrophila. Blood profile, weight gain, survival and relative survival of C. a. auratus were observed before and after the challenge test. The characteristics and types of active compounds in the extract were observed qualitatively, as well as the morphology and particle size distribution of the microcapsules. The results showed that D. auricularia extract contained phenol, tannins, steroids, terpenoids, alkaloids, and saponins. The microcapsules in all treatments were a spherical shape. The average diameter was similar to all extract treatments. The addition of D. auricularia extract microcapsules in the feed produced significantly different results on the blood profile, weight gain and survival. The highest of total leukocytes, total erythrocytes, hematocrit and blood hemoglobin were obtained in addition to 7.7 g D. auricularia extract/kg of feed with respective values ??of 7.35±0.94×104 cell.mm-3, 2.83±0.03×106 cell.mm-3, 22.43±0.03% and 6.72±0.02 g.dL-1, respectively. The highest weight gain was found in fish-fed diets contained of 7.7 g of D. auricularia extract (3.37 g). The highest relative survival and survival were found in the treatment of 7 and 7.7 g extract/kg of feed, which was 100%. This study concluded that microencapsulated with D. auricularia extract at dose 7 g extract/kg of feed was the optimum dose for C. a. auratus infected with A. hydrophila.

show abstract

Section: Introductionmentioning

confidence: 99%

Potential extracts of wedge sea hare (Dolabella auricularia) as immunostimulators in comet fish (Carassius auratus auratus) infected by Aeromonas hydrophila

2022

View full text Add to dashboard Cite

show abstract

PACAP modulates the transcription of TLR-1/TLR-5/MyD88 pathway genes and boosts antimicrobial defenses in Clarias gariepinus

Rodríguez

Velázquez

González

et al. 2021

Fish & Shellfish Immunology

View full text Add to dashboard Cite

Identification of a Potential SNP Related to the Expression of Immune Genes and Its Possible Application to Selection of WSSV-Resistant Pacific White Shrimp (<I>Litopenaeus vannamei</I>)

Basuki

Ali²,

Soelistyowati³

et al. 2023

JIPK

View full text Add to dashboard Cite

Highlight Research A molecular marker for shrimp selection in disease resistance. Single nucleotide polymorphisms (SNP) in the ALF gene strongly correlate with shrimp resistance to WSSV infection. Higher ALF gene expression in survivor shrimp. SNP as molecular marker inherited in the first generation (G1) shrimp. ARMS-PCR method successfully detect SNP in the shrimp ALF gene. Abstract The Pacific white shrimp (Litopenaeus vannamei) is Indonesia’s main export commodity, but its production is constrained by the white spot syndrome virus (WSSV). Selective breeding of disease-resistant broodstock based on single nucleotide polymorphism (SNP) in the anti-lipopolysaccharide factor (ALF) gene is an alternative strategy for solving the disease problem. This study aimed to detect the SNP g.455 A>G in the anti-lipopolysaccharide factor (ALF) shrimp gene, evaluate the correlation of SNP with WSSV-resistance trait, analyze the expression level of immunity genes and genotype frequencies of the WSSV-resistance population shrimp and analyze the SNP inheritance in the first generation of selected shrimp. A total of 120 individuals from 4 families were used to detect the SNP marker using tetra-primer amplification refractory mutation system-polymerase chain reaction (ARMS-PCR). The correlation of the SNP marker with survival rate (SR) was analyzed using a general linear model (GLM) between genotype frequencies and SR. Genotypic similarities between broodstock and pedigree were analyzed using Chi-square. SNP g.455 A>G was successfully detected using the ARMS-PCR method and had a strong correlation between the marker and SR (p-value of AA = 0.012; AG = 0.359, and GG = 0.001). The resistant population has significantly higher ALF and SOD gene expression levels and AA genotype frequency. The SNP marker was inherited, so the broodstock and pedigree have the same genotype frequencies according to chi-square analysis (χ2 = 0.46 and p-value = 0.497). These results suggested that the g.455 genotype AA could be selected to produce WSSV-resistant Pacific white shrimp.

show abstract

Short Communication: Single nucleotide polymorphism in C-type lysozyme gene and its correlation with Aeromonas hydrophila resistance in African catfish Clarias gariepinus

Cited by 3 publications

References 23 publications

Potential extracts of wedge sea hare (Dolabella auricularia) as immunostimulators in comet fish (Carassius auratus auratus) infected by Aeromonas hydrophila

Potential extracts of wedge sea hare (Dolabella auricularia) as immunostimulators in comet fish (Carassius auratus auratus) infected by Aeromonas hydrophila

PACAP modulates the transcription of TLR-1/TLR-5/MyD88 pathway genes and boosts antimicrobial defenses in Clarias gariepinus

Identification of a Potential SNP Related to the Expression of Immune Genes and Its Possible Application to Selection of WSSV-Resistant Pacific White Shrimp (<I>Litopenaeus vannamei</I>)

Contact Info

Product

Resources

About