Short exposure of intestinal epithelial cells to TNF-α and histamine induces Mac-1-mediated neutrophil adhesion independent of protein synthesis

Miyata, Ryukou; Iwabuchi, Kazuhisa; Watanabe, Sumio; Sato, Nobuhiro; Nagaoka, Isao

doi:10.1002/jlb.66.3.437

Cited by 20 publications

(21 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Our data show that none of these interventions affected PMN binding to LS174T cells in the low shear regime, suggesting the lack of L-selectin involvement under these conditions. These results are in accord with other previously published data on PMN-colon carcinoma cell interactions under static conditions (10). In distinct contrast, L-selectin appears to be critical for PMN-LS174T heteroaggregation at high shear, as evidenced by the abrogation of these heterotypic interactions upon PMN treatment with chymotrypsin or a function-blocking anti-L-selectin mAb.…”

Section: Pmn L-selectin Is Required For Optimal Pmn-ls174t Heteroaggrsupporting

confidence: 92%

“…However, use of an anti-CD11a mAb failed to reduce the extent of heterotypic interactions at a shear rate of 100 s Ϫ1 (see Fig. 4B), a finding which is in agreement with previous work performed under stationary conditions (10,11). In distinct contrast, CD11a blockade alone dramatically inhibited PMN-LS174T heteroaggregation at 800 s Ϫ1 (see Fig.…”

Section: Dependence Of Pmn-ls174t Heteroaggregation On Pmn Cd18 Integsupporting

confidence: 91%

“…Prior work has shown that PMNs interact with a variety of colon carcinoma cell lines in a CD18 (␤2 integrin)-dependent manner under static conditions (10,11). Therefore, we explored the potential involvement of CD18 in PMN-LS174T heteroaggregation under hydrodynamic shear in conjunction with chemotactic stimulation.…”

Section: Dependence Of Pmn-ls174t Heteroaggregation On Pmn Cd18 Integmentioning

confidence: 99%

“…Prior work performed under static conditions indicated that PMNs bind to cytokine-activated HT29 colon carcinoma cells in a CD11b-dependent but CD11a-and L-selectin-independent manner (10). Similarly, CD11b has been shown to mediate the attachment of fMLP-stimulated PMNs to T84 colon adenocarcinoma cells in the absence of any CD11a or CD11c contribution (11).…”

Section: Cd11b Is Sufficient To Mediate Optimal Pmn-ls174t Heteroaggrmentioning

confidence: 99%

“…Consequently, the present study was undertaken to systematically characterize the effects of hydrodynamic shear on PMN-tumor cell interactions at the molecular level using a human colon carcinoma cell model, because colon cancer is among those tumors with a propensity for hematogenous spread. The metastatic (16) sialyl Lewis x (sLe x )-bearing LS174T human colon adenocarcinoma cell line, which expresses both selectin and CD18 integrin ligands on the cell surface, was used in this study because it has been extensively characterized and widely used in a number of diverse assays (10,12,17,18). For comparison purposes, we also looked at the nonmetastatic (16,19) HCT-8 colon adenocarcinoma cell line expressing minimal levels of sLe x (sLe x -low HCT-8 cells) (16,19).…”

mentioning

confidence: 99%

See 4 more Smart Citations

Hydrodynamic Shear Regulates the Kinetics and Receptor Specificity of Polymorphonuclear Leukocyte-Colon Carcinoma Cell Adhesive Interactions

Jadhav

Bochner

Κωνσταντόπουλος

2001

The Journal of Immunology

View full text Add to dashboard Cite

The ability of tumor cells to metastasize hematogenously is regulated by their interactions with polymorphonuclear leukocytes (PMNs). However, the mechanisms mediating PMN binding to tumor cells under physiological shear forces remain largely unknown. This study was designed to characterize the molecular interactions between PMNs and tumor cells as a function of the dynamic shear environment, using two human colon adenocarcinoma cell lines (LS174T and HCT-8) as models. PMN and colon carcinoma cell suspensions, labeled with distinct fluorophores, were sheared in a cone-and-plate rheometer in the presence of the PMN activator fMLP. The size distribution and cellular composition of formed aggregates were determined by flow cytometry. PMN binding to LS174T cells was maximal at 100 s−1 and decreased with increasing shear. At low shear (100 s−1) PMN CD11b alone mediates PMN-LS174T heteroaggregation. However, L-selectin, CD11a, and CD11b are all required for PMN binding to sialyl Lewisx-bearing LS174T cells at high shear (800 s−1). In contrast, sialyl Lewisx-low HCT-8 cells fail to aggregate with PMNs at high shear conditions, despite extensive adhesive interactions at low shear. Taken together, our data suggest that PMN L-selectin initiates LS174T cell tethering at high shear by binding to sialylated moieties on the carcinoma cell surface, whereas the subsequent involvement of CD11a and CD11b converts these transient tethers into stable adhesion. This study demonstrates that the shear environment of the vasculature modulates the dynamics and molecular constituents mediating PMN-tumor cell adhesion.

show abstract

Section: Pmn L-selectin Is Required For Optimal Pmn-ls174t Heteroaggrsupporting

confidence: 92%

Section: Dependence Of Pmn-ls174t Heteroaggregation On Pmn Cd18 Integsupporting

confidence: 91%

Section: Dependence Of Pmn-ls174t Heteroaggregation On Pmn Cd18 Integmentioning

confidence: 99%

Section: Cd11b Is Sufficient To Mediate Optimal Pmn-ls174t Heteroaggrmentioning

confidence: 99%

mentioning

confidence: 99%

See 3 more Smart Citations

Hydrodynamic Shear Regulates the Kinetics and Receptor Specificity of Polymorphonuclear Leukocyte-Colon Carcinoma Cell Adhesive Interactions

Jadhav

Bochner

Κωνσταντόπουλος

2001

The Journal of Immunology

View full text Add to dashboard Cite

show abstract

Regulatory Effect of Histamine on the Barrier Function of Intestinal Mucosal

Duan

Chen

Alexander

2010

Journal of Gastrointestinal Surgery

View full text Add to dashboard Cite

show abstract

Local inflammatory events induced by Bothrops atrox snake venom and the release of distinct classes of inflammatory mediators

Moreira

Dos-Santos

Nascimento

et al. 2012

Toxicon

View full text Add to dashboard Cite

Short exposure of intestinal epithelial cells to TNF-α and histamine induces Mac-1-mediated neutrophil adhesion independent of protein synthesis

Cited by 20 publications

References 44 publications

Hydrodynamic Shear Regulates the Kinetics and Receptor Specificity of Polymorphonuclear Leukocyte-Colon Carcinoma Cell Adhesive Interactions

Hydrodynamic Shear Regulates the Kinetics and Receptor Specificity of Polymorphonuclear Leukocyte-Colon Carcinoma Cell Adhesive Interactions

Regulatory Effect of Histamine on the Barrier Function of Intestinal Mucosal

Local inflammatory events induced by Bothrops atrox snake venom and the release of distinct classes of inflammatory mediators

Contact Info

Product

Resources

About