Short ingestion tests as alternative proposal for conventional range finding assays with Thamnocephalus platyurus and Brachionus calyciflorus

Abstract

ABSRACT:The goal of this study was to evaluate whether short 1 h sublethal assays may predict the results of 24 h lethality assays with rotifers Brachionus calyciflorus and anostracan crustaceans Thamnocephalus platyurus. The test bionts were hatched from cysts. Inhibition of ingestion was observed after 15 min of incubation of rotifers and crustaceans with the suspension of carmine and latex beads, respectively. Nine compounds with different modes of action were used as toxicants zinc ions, sodium dodecyl sulphate, p-nitrophenol, 3, 5-dichlorophenol and pharmaceuticals propranolol, fluoxetine, abamectin, doramectin and ivermectin. The toxicity values observed in the ingestion tests were very close to the mortality values over a wide range of toxicity from a low toxic surfactant to very toxic avermectins. The ratio between the 1 h EC 50 's in the ingestion test and the 24 h LC 50 's in the lethality test was below 2 in all cases for rotifers, and 7 in 9 cases for crustaceans. The toxicity of zinc and 3,5-dichlorophenol in the Thamnotoxkit F™ was 15-fold higher and 10 fold lower than in the ingestion test, respectively. The 24 h LC 50 values are within the range of 25-400 % of the 1 h EC 50 values for almost all toxicants tested with the exception of p-nitrophenol for B. calyciflorus and zinc and 3, 5-dichlorophenol for T. platyurus. Short, 1 h ingestion assays Rotoxrapid and Rapidtoxkit are good predictors of the mortality over the next 24 h and can be used as a range finding tests for representatives of pharmaceuticals and surfactants.

Adverse effects of the SSRI antidepressant sertraline on early life stages of marine invertebrates

“…Alterations in barnacle's locomotion may result in irreversible modifications that may contribute to the death of the animal. In rotifers, swimming behavior has been shown to play an important role in several processes like feeding or predator avoidance (Larsen et al, 2008;Yúfera, 2007); furthermore, crustaceans and rotifers are an important link between nanoplankton and macrozoplankton (Jawecki et al, 2011). Some research has been focused on Brachionus spp.…”

Influence of Selected Antidepressants on the Ciliated Protozoan Spirostomum ambiguum: Toxicity, Bioaccumulation, and Biotransformation Products

“…0.6 mg/L for Thamnocephalus platyurus [27] and 48-h EC50 of 0.92 mg/L for Daphnia magna [28]. Slightly lower toxicity was reported for fluoxetine, ranging from 0.23 and 0.82 mg/L for C. dubia and D. magna [12] to 0.85 mg/L for T. platyurus [29]. Contrary to the previous two antidepressants, paroxetine was 10-fold less toxic to D. magna (6.3 mg/L) [28] than to C. dubia (0.58 mg/L) [26].…”

Integrated Ecological Risk Assessment of the Agricultural Area under a High Anthropopressure Based on Chemical, Ecotoxicological and Ecological Indicators

“…Rapidtoxkit is an indicator test for detecting the presence of toxic substances using crustacean larvae Thamnocephalus platyurus [22,32,33], which take up their food by filtration from the surrounding solution. The rate and level of filtration decrease or, in extreme cases, stop completely after the exposure of T. platyurus to toxic substances.…”