Short peptide epitope design from hantaviruses causing HFRS

Abstract

Several genotypes of the hantavirus cause hemorrhagic fever with renal syndrome (HFRS) and is an important public health problem worldwide. There is now growing interest to develop subunit vaccines especially focused to elicit cytotoxic T lymphocyte responses which are important against viral infection. We identified candidate T-cell epitopes that bind to Class I HLA supertypes towards identifying potential subunit vaccine entity. These epitopes are conserved in all 5 hantavirus genotypes of HFRS (Hantaan, Dobrava- Belgrade, Seoul, Gou virus and Amur). The epitopes identified from S and M segment genomes were analyzed for human proteasome cleavage, transporter associated antigen processing (TAP) efficiency and antigenicity using bioinformatic approaches. The epitope MRNTIMASK which had the two characteristics of high proteasomal cleavage score and TAP score, also had high antigenicity score. Our results indicate that this epitope from the nucleocapsid protein may be considered the most favorable moiety for the development of subunit peptide vaccine.

Exploring the out of sight antigens of SARS-CoV-2 to design a candidate multi-epitope vaccine by utilizing immunoinformatics approaches

“…The MAPPP ( http://www.mpiib-berlin.mpg.de/MAPPP/cleavage.html ) [38] and NetChop ( http://tools.iedb.org/netchop/ ) were used for predicting the proteasomal processing [39] . In addition, the binding affinity of the multi-epitope vaccine with the TAP (transporter associated with antigen processing) was investigated by the TAPPred ( http://crdd.osdd.net/raghava/tappred/index.html ) server [40] .…”

In Silico Analysis of Synaptonemal Complex Protein 1 (SYCP1) and Acrosin Binding Protein (ACRBP) Antigens to Design Novel Multiepitope Peptide Cancer Vaccine Against Breast Cancer

“…NetChop (http://tools .iedb.org/netch op/) and MAPPP (http:// www.mpiib -berli n.mpg.de/MAPPP / cleavage.html) (Subramanian and Chinnappan 2013) were employed for predicting the proteasomal processing. In addition, TAPPred (http:// crdd.osdd.net/ragha va/tappr ed/index .html) (Sankar et al 2017) was used to evaluate the binding affinity of the peptides with the TAP transporter.…”

Integrative Analysis of HTNV Glycoprotein Derived MHC II Epitopes by In Silico Prediction and Experimental Validation

“…However, it is well known that inactivated vaccines do not provide long-lasting immunity without multiple boosters ( Song et al., 2016 ). Therefore, many studies have explored novel candidate vaccines against hantavirus with new epitope designs ( Sankar et al., 2017a ; Sankar et al., 2017b ; Conte et al., 2019 ) and different methods evaluating efficacy ( Zhao et al., 2012 ; Hooper et al., 2013 ; Ma et al., 2016 ). These studies mainly focused on CD8+ T cells and B cell-mediated immunity.…”