Short-term alanyl-glutamine dipeptide pretreatment in liver ischemia–reperfusion model: Effects on microcirculation and antioxidant status in rats

Szíjártó, Attila; Hahn, Oszkár; Batmunkh, Enkhjargal; Stangl, Rita; Kiss, András; Lotz, Gábor; Schaff, Zsuzsa; Váli, László; Blázovics, Anna; Gerö, Domokos; Szabó, Csaba; Kempler, Péter; Harsányi, László

doi:10.1016/j.clnu.2007.06.011

Cited by 24 publications

(16 citation statements)

References 27 publications

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“…Nesses experimentos, a administração foi realizada de 3-72 h antes da isquemia em infusão intravenosa contínua (Harward et al 1994, Jia et al 2006, Szijártó et al 2007). Entretanto, é fundamental ressaltar que essa utilização é praticamente inviável em casos de eqüinos com cólica, uma vez que seria necessário prever sua ocorrência para iniciar o tratamento com a glutamina.…”

Section: Discussionunclassified

“…A suplementação com glutamina foi benéfica na regeneração da mucosa intestinal e na cicatrização cutânea em humanos portadores de queimaduras e, em condições experimentais, em casos de injúria e reperfusão intestinal, hepática e periférica em ratos (Harward et al 1994, Peng et al 2004, Jia et al 2006, Murphy et al 2007, Szijártó et al 2007. A glutamina foi indicada em casos de transplantes ósseos e cirurgias intestinais em humanos (Fuchs & Bod 2006) e, em eqüinos como suplemento nutricional para atletas (Harris et al 2006).…”

Section: Introductionunclassified

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Utilização da glutamina no tratamento de lesões de isquemia e reperfusão no jejuno em eqüinos

et al. 2009

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Sob anestesia geral e com controle da pressão arterial, 12 eqüinos foram submetidos simultaneamente a modelos de isquemia venosa e distensão intraluminal em segmentos do jejuno por 2 h, seguidos de 12 h de reperfusão. Seis eqüinos foram tratados com glutamina a 2% (50mg/kg) por via intravenosa e seis com solução fisiológica em igual volume, 1 h após o início da isquemia e às 6h de reperfusão. Foram colhidas amostras de tecido intestinal antes e com 2 h de isquemia e com 2 e 12 h de reperfusão para avaliações histopatológica e ultraestrutural, mensuração da área e número das vilosidades por mm² e atividade de mieloperoxidade (MPO). Não foram encontradas diferenças entre os grupos controle e tratado. Concluiu-se que a glutamina, nas condições deste trabalho, não atenua lesões de isquemia e reperfusão intestinal em eqüinos.

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Section: Discussionunclassified

Section: Introductionunclassified

Utilização da glutamina no tratamento de lesões de isquemia e reperfusão no jejuno em eqüinos

et al. 2009

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“…Within the sequelae of events, proinflammatory mediators such as TNF-α and IL-1β are key players, causing the microvascular dysfunction and perfusion failure. 8 Many imaging techniques have been previously used to study the hepatic microcirculation, such as use of gamma-emitting radionuclide-labeled microspheres, 4,5 laser Doppler flowmetry, 6 and computed tomography. 7 However, CEUS has still not been reported in the monitoring of liver microcirculation after ischemia-reperfusion injury, although it is a real-time and radiation-free method versus the other techniques.…”

Section: Discussionmentioning

confidence: 99%

“…1,2 Regarding the liver, hepatic ischemia-reperfusion injury is commonly a consequence of prolonged portal triad clamping followed by reperfusion during surgical resection, performed as an elective preplanned procedure or as an emergency maneuver, to control excessive bleeding from the cut hepatic surface. 2,3 Many techniques have been previously used for the study of hepatic microcirculation, such as the use of gamma-emitting radionuclide-labeled microspheres, 4,5 laser Doppler flowmetry, 6 and computed tomography. 7 However, as a method in monitoring microcirculation in many other organs and tissues, contrast-enhanced ultrasonography (CEUS) has not still been mentioned in this area.…”

Section: Introductionmentioning

confidence: 99%

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2016

Exp Clin Transplant

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Objectives: Our objective was to evaluate the effects of contrast-enhanced ultrasonography in monitoring microcirculation after rat liver ischemia-reperfusion injury. Materials and Methods: Male Wistar rats (n = 36) were divided into sham-operated and ischemia-reperfusion groups. Rats in the ischemia-reperfusion groups underwent normothermic liver ischemia for 15 minutes followed by 1, 6, or 24 hours of reperfusion. At different time points, contrast-enhanced ultrasonography was performed to determine peak intensity in monitoring hepatic microcirculation. In addition, serum levels of alanine aminotransferase, aspartate aminotransferase, tumor necrosis factor α, and interleukin 1β levels were measured. Histopathologic changes were also observed. Results: One hour after reperfusion, peak intensity values decreased, and serum levels of alanine aminotransferase, tumor necrosis factor α, and interleukin 1β increased significantly in the ischemiareperfusion group compared with the sham-operated group. Histology results showed mild injury. Six hours after reperfusion, peak intensity values decreased continuously, serum levels of alanine aminotransferase, tumor necrosis factor α, and interleukin 1β decreased, and aspartate aminotransferase levels increased. Histology results showed severe injury compared with 1 hour after reperfusion. Twenty-four hours after reperfusion, peak intensity values increased, alanine aminotransferase and aspartate aminotransferase levels decreased, and histology results showed moderate injury compared with 6 hours after reperfusion. Peak intensity values were negatively correlated to alanine aminotransferase (P < .05; γ = -0.38) and aspartate aminotransferase (P < .01; γ = -0.78) levels. Conclusions: Microcirculation dysfunction after liver ischemia-reperfusion injury can be monitored by contrast-enhanced ultrasonography. The perfusion of contrast agents negatively correlates to the severity of injuries.

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“…Szoros értelemben véve nem kondicionálószer a glutamin, azonban számos jótékony hatá-sánál fogva régóta ismeretes pozitív hatása számos katabolikus állapot kapcsán [35]. Így preoperatív ("későbbi": 3 óra; "korábbi": 24 óra) alkalmazásának lehetőségét kutatócsoportunk máj I-R károsodása kapcsán vizsgálta [36,37]. A kezelés szignifi kánsan csökkentette a máj I-R károsodását, amelynek hátterében állhat egyfelől a nekrotikus folyamatok helyett az apoptózis -TUNEL-festéssel és kaszpáz-3 immunhisztokémiával igazolt -serkentése.…”

Section: Kondicionáló Eljárások éS Máj Ischaemiareperfúzió -áLlatkíséunclassified

Szabad gyökök és a máj ischaemiás-reperfúziós károsodása

Szíjártó

2015

Orvosi Hetilap

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Az ischaemiás-reperfúziós károsodás kiemelkedő jelentősége számos szerv és klinikai szituáció esetén jól bizonyított. A posztoperatív komplikációk prevalenciáját, a célszerv sérülését és a szisztémás szövődmények előfordulását jelentő-sen meghatározó károsodás mediálásában központi szerep jut a szabad gyököknek. A szabadgyök-stresszre kimondottan érzékeny máj anatómiailag és patofi ziológiailag speciális ischaemiás-reperfúziós károsodása májsebészeti szempontból kulcsfontosságú. A reperfúziós károsodás mérséklésére számos kondicionáló eljárás (adaptív technikák, kémiai vegyületek) ismert. Jelen összefoglaló célja az ischaemiás-reperfúziós károsodás mérséklésére irányuló technikák áttekintése a szerző kutatócsoportjának eddigi munkássága (ischaemiás prekondicionálás, -perkondicionálás, glutaminszupplementáció, adenozin-, inozin-, levosimendan-, poli-ADP-ribóz polimerázgátlás szerepe) alapján, külö-nös tekintettel a máj ischaemiás-reperfúziós károsodására, valamint a szabad gyökök abban betöltött patofi ziológiai szerepére. Orv. Hetil., 2015, 156(47), 1904-1907. Kulcsszavak: ischaemiás-reperfúziós károsodás, máj, szabad gyök, ischaemiás prekondicionálás Free radicals and hepatic ischemia-reperfusionThe critical importance of the ischemic-reperfusive injury is well documented with regards to numerous organs and clinical conditions. Oxygen free radicals play a central role in the mediation of the injury, which dominantly infl uences the prevalence of postoperative complications, (long term) organ damage, and the potential manifestation of systemic reactions. The both anatomically and pathophysiologically unique ischemic-reperfusive injury of the liver, which is expressively vulnerable to free radicals, is of utmost importance in liver surgery. Several techniques (adaptive maneuvers, chemical agents) are known to ameliorate the reperfusive injury. Based on the prior research of the workgroup of the author, the aim of the current article is to overview the set of measures capable of attenuating ischemic-reperfusive injury (ischemic preconditioning, -perconditioning, administration of adenosine, -inosine, -levosimendan, and -poly-ADP-ribose-polymerase inhibitor), with special attention to the ischemic-reperfusive injury of the liver, as well as the special pathophysiological role of free radicals in mediating hepatic damage. Keywords: ischemia-reperfusion injury, liver, free radicals, ischemic preconditioningSzijártó, A. [Free radicals and hepatic ischemia-reperfusion]. Orv. Hetil., 2015, 156(47), 1904-1907. (Beérkezett: 2015 elfogadva: 2015. szeptember 23.) Rövidítések ADP = adenozin-difoszfát; ALP = alkalikus foszfatáz; ALT = alanin-aminotranszferáz; AMP = adenozin-monofoszfát; ATP = adenozin-triszfoszfát; CARS = compensatory anti-infl ammatory response syndrome; CX = (circumfl ex artery) bal szívkoszorúér körbefutó ága; DAMP = danger-associated molecular pattern; DNS = dezoxiribonukleinsav; GSH = glutation redukált forma; I-R = ischaemia-reperfúzió; ic. = intracelluláris; IFN-γ = interferon-γ; IL-1β = interleuk...

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Short-term alanyl-glutamine dipeptide pretreatment in liver ischemia–reperfusion model: Effects on microcirculation and antioxidant status in rats

Cited by 24 publications

References 27 publications

Utilização da glutamina no tratamento de lesões de isquemia e reperfusão no jejuno em eqüinos

Utilização da glutamina no tratamento de lesões de isquemia e reperfusão no jejuno em eqüinos

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Szabad gyökök és a máj ischaemiás-reperfúziós károsodása

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