Short-term antioxidative responses of 15 microalgae exposed to excessive irradiance including ultraviolet radiation

Janknegt, Paul J.; Graaff, C. Marco de; Poll, Willem H. van de; Visser, Ronald J. W.; Rijstenbil, J.W.; Buma, Anita G. J.

doi:10.1080/09670260902943273

Cited by 47 publications

(35 citation statements)

References 84 publications

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“…We did observe a slowing of PSII repair under sustained high WL. Therefore, our results indicate that the enhanced photoinactivation under moderately high BL or high WL was not mediated through higher ROS toxicity, again consistent with Nishiyama et al (2006) and Tyystjarvi (2008) and distinct from the results of Janknegt et al (2009), who found that under yet higher full sunlight, differential ROS toxicity contributed significantly to differential photophysiology of phytoplankton taxa. We speculate that the induction of NPQs might preempt the production of ROS, although we have no direct evidence for this linkage as yet.…”

Section: Discussionsupporting

confidence: 90%

“…Overexcitation of PSII can lead to the production of reactive oxygen species (ROS; Mü ller et al, 2001), causing damage to the photosynthetic apparatus (Nishiyama et al, 2006) and leading potentially to cell death (Janknegt et al, 2009). Diatoms, like all oxygenic photoautotrophs, are subject to photoinactivation of their PSII reaction centers (Nagy et al, 1995;Six et al, 2007;Edelman and Mattoo, 2008).…”

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confidence: 99%

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Distinctive Photosystem II Photoinactivation and Protein Dynamics in Marine Diatoms

Cockshutt

McCarthy

et al. 2011

Plant Physiology

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1G8 (A.M.C., A.M.) Diatoms host chlorophyll a/c chloroplasts distinct from green chloroplasts. Diatoms now dominate the eukaryotic oceanic phytoplankton, in part through their exploitation of environments with variable light. We grew marine diatoms across a range of temperatures and then analyzed their PSII function and subunit turnover during an increase in light to mimic an upward mixing event. The small diatom Thalassiosira pseudonana initially responds to increased photoinactivation under blue or white light with rapid acceleration of the photosystem II (PSII) repair cycle. Increased red light provoked only modest PSII photoinactivation but triggered a rapid clearance of a subpool of PsbA. Furthermore, PsbD and PsbB content was greater than PsbA content, indicating a large pool of partly assembled PSII repair cycle intermediates lacking PsbA. The initial replacement rates for PsbD (D2) were, surprisingly, comparable to or higher than those for PsbA (D1), and even the supposedly stable PsbB (CP47) dropped rapidly upon the light shift, showing a novel aspect of rapid protein subunit turnover in the PSII repair cycle in small diatoms. Under sustained high light, T. pseudonana induces sustained nonphotochemical quenching, which correlates with stabilization of PSII function and the PsbA pool. The larger diatom Coscinodiscus radiatus showed generally similar responses but had a smaller allocation of PSII complexes relative to total protein content, with nearly equal stiochiometries of PsbA and PsbD subunits. Fast turnover of multiple PSII subunits, pools of PSII repair cycle intermediates, and photoprotective induction of nonphotochemical quenching are important interacting factors, particularly for small diatoms, to withstand and exploit high, fluctuating light.Diatoms are oxygenic photoautotrophs whose cell structures and chlorophyll a/c chloroplasts are evolutionarily, structurally, and functionally distinct from the green lineage with chlorophyll a/b chloroplasts (Armbrust et al., 2004;Wilhelm et al., 2006;Larkum et al., 2007). Over the past 100 million years , diatoms have become nearly ubiquitous, accounting for approximately 20% of global primary productivity (Field et al., 1998). They are currently by far the most successful group of eukaryotic phytoplankton, not only in terms of primary production but also in their number of species (Medlin and Kaszmarska, 2004), which span a wide cell size range (Beardall et al., 2009). Therefore, diatoms functionally dominate the phytoplankton population (Wilhelm et al., 2006), particularly in turbulent coastal waters where they are exposed to frequent and large fluctuations in light due to fast vertical mixing through steep photic zone light gradients (Long et al., 1994;MacIntyre et al., 2000).In response to a sudden increase in irradiance, diatoms can dissipate excess light energy through distinct mechanisms of nonphotochemical quenching (NPQ; Lavaud et al., 2004;Eisenstadt et al., 2008;Grouneva et al., 2009;Bailleul et al., 2010;Park et al., 2010;Zhu and Green, ...

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Section: Discussionsupporting

confidence: 90%

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confidence: 99%

Distinctive Photosystem II Photoinactivation and Protein Dynamics in Marine Diatoms

Cockshutt

McCarthy

et al. 2011

Plant Physiology

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“…It is thus likely that iodide mobilization might contribute to the pool of antioxidant mechanisms acting in response to elevated ROS production due to high photosynthetic activity during exponential growth. The nature of the response of marine microalgae to irradiance is highly species-specific (Janknegt et al, 2009) and iodide could be playing a yet uncovered function at this regard. The later increase of dio in the declining phase suggests an additional role for iodide in other cellular processes not directly linked to photosynthesis.…”

Section: Discussionmentioning

confidence: 99%

“…Cells then need to rearrange mobilization of oxidative defense mechanisms including peroxidases, SODs, and other antioxidant defenses such as carotenoids (Blokhina et al, 2003). Although previous studies in diverse microalgae demonstrated absence of correlation between irradiance intensity and SOD activity (Janknegt et al, 2009), an increased SOD activity in the stationary growth phase associated to oxidative stress has been reported (Sigaud-Kutner et al, 2002). Moreover, SOD, catalase and APx activities are also activated under nitrogen depletion to cope with the physiological stress induced by such condition (Yilancioglu et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

Characterization of Iodine-Related Molecular Processes in the Marine Microalga Tisochrysis lutea (Haptophyta)

et al. 2018

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Iodine metabolism is essential for the antioxidant defense of marine algae and in the biogeochemical cycle of iodine. Moreover, some microalgae can synthetize thyroid hormone-like compounds that are essential to sustain food webs. However, knowledge regarding iodine-related molecular processes in microalgae is still scarce. In this study, a de novo transcriptome of Tisochrysis lutea cultured under high iodide concentrations (5 mM) was assembled using both long and short reads. A database termed IsochrysisDB was established to host all genomic information. Gene expression analyses during microalgal growth showed that most of the antioxidant-(aryl, ccp, perox, sod1, sod2, sod3, apx3, ahp1) and iodide-specific deiodinase (dio) genes increased their mRNA abundance progressively until the stationary phase to cope with oxidative stress. Moreover, the increase of dio mRNA abundance in aging cultures indicated that this enzyme was also involved in senescence. Cell treatments with iodide modified the expression of perox whereas treatments with iodate changed the transcript levels of gpx1 and ccp. To test the dependence of perox on iodide, microalgae cells were treated with hydrogen peroxide (H 2 O 2 ) either in presence or absence of iodide observing that several genes related to reactive oxygen species (ROS) deactivation (perox, gpx1, apx2, apx3, ahp1, ahp2, sod1, sod3, and aryl) were transcriptionally activated although with some temporal differences. However, only the expression of perox was dependent on iodide levels indicating this enzyme, acquired by horizontal gene transfer (HGT), could act as a haloperoxidase. All these data indicate that T. lutea activates coordinately the expression of antioxidant genes to cope with oxidative stress. The identification of a phase-regulated deiodinase and a novel haloperoxidase provide new clues about the origin and evolution of thyroid signaling and the antioxidant role of iodine in the marine environment.

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“…Little is currently known about the ROS scavenging capacity of different Symbiodinium subclades [24,59] and the knowledge about the role of glutathione in the antioxidant system of Symbiodinium is still fragmentary [60]; however, we do know that the intracellular location of ROS formation dictates which antioxidant might increase at the site of production [61]. Similarly, molecules such as DMS can diffuse through biological membranes and reach any cellular compartment [20,23] thereby interacting with the same ROS molecules.…”

Section: Discussionmentioning

confidence: 99%

Dimethylsulfoniopropionate, superoxide dismutase and glutathione as stress response indicators in three corals under short-term hyposalinity stress

et al. 2016

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Corals are among the most active producers of dimethylsulfoniopropionate (DMSP), a key molecule in marine sulfur cycling, yet the specific physiological role of DMSP in corals remains elusive. Here, we examine the oxidative stress response of three coral species (Acropora millepora, Stylophora pistillata and Pocillopora damicornis) and explore the antioxidant role of DMSP and its breakdown products under short-term hyposalinity stress. Symbiont photosynthetic activity declined with hyposalinity exposure in all three reef-building corals. This corresponded with the upregulation of superoxide dismutase and glutathione in the animal host of all three species. For the symbiont component, there were differences in antioxidant regulation, demonstrating differential responses to oxidative stress between the Symbiodinium subclades. Of the three coral species investigated, only A. millepora provided any evidence of the role of DMSP in the oxidative stress response. Our study reveals variability in antioxidant regulation in corals and highlights the influence life-history traits, and the subcladal differences can have on coral physiology. Our data expand on the emerging understanding of the role of DMSP in coral stress regulation and emphasizes the importance of exploring both the host and symbiont responses for defining the threshold of the coral holobiont to hyposalinity stress.

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Short-term antioxidative responses of 15 microalgae exposed to excessive irradiance including ultraviolet radiation

Cited by 47 publications

References 84 publications

Distinctive Photosystem II Photoinactivation and Protein Dynamics in Marine Diatoms

Distinctive Photosystem II Photoinactivation and Protein Dynamics in Marine Diatoms

Characterization of Iodine-Related Molecular Processes in the Marine Microalga Tisochrysis lutea (Haptophyta)

Dimethylsulfoniopropionate, superoxide dismutase and glutathione as stress response indicators in three corals under short-term hyposalinity stress

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