Short-term bioaccumulation, circulation and metabolism of estradiol-17β in the oyster Crassostrea gigas

Curieux-Belfond, Olivier Le; Fiévet, Bruno; Séralini, Gilles‐Éric; Mathieu, Michel

doi:10.1016/j.jembe.2005.04.027

Cited by 34 publications

(16 citation statements)

References 20 publications

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“…This route of exposure, that allows for direct administration of estrogens into the animal open circulatory system leading to distribution from the blood (hemolymph) to the tissues, including the hepatopancreas, has been largely utilised for evaluating the effects of natural and environmental estrogens in bivalve molluscs (Gagnè et al, 2001;Canesi et al, 2005Canesi et al, , 2006Canesi et al, , 2007Le Curieux-Belfond et al, 2005;Gauthier-Clerc et al, 2006;. In Mytilus sp., exogenous E 2 added to the seawater (200-2000 ng/l) is taken up and progressively metabolised (within 7 days) to an esterified, inactive form that is stored in the tissues, this indicating that mussels can tightly regulate the concentration of free E 2 (Janer et al, 2005).…”

Section: Discussionmentioning

confidence: 99%

“…In bivalves, direct administration into the blood (hemolymph) by injection into the adductor muscle sinus has been widely utilised for identifying the possible targets of both natural and environmental estrogens (Gagnè et al, 2001;Canesi et al, 2005Canesi et al, , 2006Canesi et al, , 2007Le Curieux-Belfond et al, 2005;GauthierClerc et al, 2006;. The concentrations of BPA (ng/g dry weight/mussel) were in the same range as those previously shown to affect mussel immune parameters in the same experimental conditions (Canesi et al, 2005) and comparable to environmental exposure levels (Pojana et al, 2004 A c c e p t e d M a n u s c r i p t 6…”

Section: Page 4 Of 32mentioning

confidence: 99%

“…In bivalve molluscs, injection into the adductor muscle sinus allows for direct administration of estrogens into the animal open circulatory system leading distribution from the blood (hemolymph) to the tissues, including the hepatopancreas (Le Curieux-Belfond et al, 2005). This method has been largely utilised for evaluating the effects of natural and environmental estrogens in bivalves (Gagnè et al, 2001;Canesi et al, 2005Canesi et al, , 2006Canesi et al, , 2007Gauthier-Clerc et al, 2006;.…”

Section: Animals and Treatmentsmentioning

confidence: 99%

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Bisphenol-A alters gene expression and functional parameters in molluscan hepatopancreas

Canesi

Borghi

Ciacci

et al. 2007

Molecular and Cellular Endocrinology

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This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.A c c e p t e d M a n u s c r i p t A c c e p t e d M a n u s c r i p t 2 AbstractBisphenol A (BPA) is a well-known xenoestrogen in mammalian systems that can affect reproduction also in aquatic organisms. In this work the possible effects of BPA were investigated in the hepatopancreas of the bivalve mollusc Mytilus galloprovincialis: mussels were injected with different amounts of BPA (3-60 ng/g dw tissue) and tissues sampled at 24 h post-injection. Overall, these data indicate that BPA, at environmentally relevant concentrations, can have both estrogen-like and distinct effects in invertebrates like in vertebrates. Expression of different

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Section: Discussionmentioning

confidence: 99%

Section: Page 4 Of 32mentioning

confidence: 99%

Section: Animals and Treatmentsmentioning

confidence: 99%

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Bisphenol-A alters gene expression and functional parameters in molluscan hepatopancreas

Canesi

Borghi

Ciacci

et al. 2007

Molecular and Cellular Endocrinology

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“…Transformation and synthesis of steroids by microalgae or bacteria are possible (Pollio et al 1994;Panter et al 1999). Moreover, steroid and steroid-like compounds may be present in the environment and Le Curieux-Belfond et al (2005) investigated the bioaccumulation of estradiol-17b by the oyster Crassostrea gigas during in vivo experiments: in less than 48 h, estradiol-17b from seawater is concentrated up to 31 times in the soft tissues of oysters.…”

Section: Occurrence and Titer Changesmentioning

confidence: 99%

Steroids in aquatic invertebrates

2007

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Steroid molecules are present in all invertebrates, and some of them have established hormonal roles: this is the case for ecdysteroids in arthropods and, to a lesser extent, for vertebrate-type steroids in molluscs. Steroids are not only hormones, they may also fulfill many other functions in chemical communication, chemical defense or even digestive physiology. The increasing occurrence of endocrine disruption problems caused by environmental pollutants, which interfere in particular with reproductive physiology of vertebrates but also of invertebrates has made necessary to better understand the endocrine physiology of the latter and the role of steroids in these processes. So many attempts are being made to better understand the endocrine roles of steroids in arthropods and molluscs, and to establish whether they also fulfill similar functions in other invertebrate phyla. At the moment, both the precise identification of these steroids, the determination of their origin (endogenous versus exogenous) and of their mechanism of action are under active investigation. This research takes profit of the development of genome sequencing programs on many invertebrate species, which allow the identification of receptors and/or biosynthetic enzymes, when related to their vertebrate counterparts, but the story is not so simple, as will be exemplified by estrogen receptors of molluscs.

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“…Preston et al (2000) In principle, however, there is a need to build a coherent picture of the reproductive endocrinology processes in a representative macroinvertebrate species from a given taxa, together with an understanding of the toxicological processes around xenobiotic absorption, distribution, metabolism and excretion (ADME). Le Curieux-Belfond et al (2005) and Wintermyer et al (2005) have both shown an impressive practical example of invertebrate ADME work using Pacific oysters and Eastern oysters, respectively. The principles of ADME are shown in Fig.…”

Section: Research Recommendation 2-prioritisation Of Selected Laboratmentioning

confidence: 99%

Small is useful in endocrine disrupter assessment—four key recommendations for aquatic invertebrate research

Hutchinson

2007

Ecotoxicology

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As we enter the 21st "biocentury", with issues such as biodiversity and biotechnology growing in public profile, it is important to reflect on the immense ecological, medical and economic importance of invertebrates. Efforts to understand the diverse biology of invertebrates come from many directions, including Nobel Prize winning developmental biology, research to control insects that threaten human health and food supplies, aquaculture opportunities and also within ecotoxicology. In the latter context, this special journal volume highlights the importance of addressing endocrine disruption in aquatic invertebrates, from molecular and cellular biomarkers to population-relevant adverse effects. The contributors to this special volume have provided an excellent assessment of both the fundamental endocrinology and applied ecotoxicology of many aquatic invertebrate groups. On the premise that reproductive success is ultimately the vital population parameter, this chapter gives a personal view of key gaps in knowledge in invertebrate reproductive and developmental endocrinology and ecotoxicology. Based on current knowledge, there are four key issues that need to be prioritised within aquatic ecotoxicology: (1) a wider assessment of the reproductive status of invertebrates in both freshwater and coastal ecosystems; (2) prioritisation of laboratory studies in OECD and other regulatory test organisms, including basic endocrinology and ADME (absorption, distribution, metabolism and excretion) research; (3) development and validation of mechanistic biomarkers that can be used as "signposts" to help prioritise species and chronic test endpoint selection, and help link data from laboratory and field studies; and (4) develop a comparative invertebrate toxicology database utilising the prioritised reference chemicals from the EDIETA workshop, encompassing the diverse modes-of-action pertinent to endocrine disrupter testing in both aquatic arthropod and non-arthropod invertebrates.

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Short-term bioaccumulation, circulation and metabolism of estradiol-17β in the oyster Crassostrea gigas

Cited by 34 publications

References 20 publications

Bisphenol-A alters gene expression and functional parameters in molluscan hepatopancreas

Bisphenol-A alters gene expression and functional parameters in molluscan hepatopancreas

Steroids in aquatic invertebrates

Small is useful in endocrine disrupter assessment—four key recommendations for aquatic invertebrate research

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