Short term culture from solid tissues of fishes

Fenocchio, Alberto Sérgio; Vênere, Paulo César; Cesar, A.C.G.; Dias, Ana Lúcia; Bertollo, L.A.C.

doi:10.1080/00087114.1991.10797181

Cited by 67 publications

(44 citation statements)

References 13 publications

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“…In this way, P. joselimaianus (Weber, 1991) (three females, four males and one unidentified sex) were sampled in the lago Quatro Bocas (15º23'20.1''S, 51º42'45.9''W), near the city of Araguaiana, Mato Grosso, Brazil. Voucher specimens were deposited in the fish collection of the Instituto de Ciências e Letras do Médio Araguaia, Universidade Federal de Mato Grosso, Brazil (ICLMA434 Chromosomal preparations were obtained either from kidney cells after in vivo colchicine treatment (Bertollo et al, 1978), or from short-term cell culture (Fenocchio et al, 1991). These were Giemsa stained for conventional analysis.…”

Section: Methodsmentioning

confidence: 99%

Karyotype description of three species of Loricariidae (Siluriformes) and occurrence of the ZZ/ZW sexual system in Hemiancistrus spilomma Cardoso & Lucinda, 2003

2006

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The neotropical freshwater systems have a high number of catfish species (Siluriformes), and many of those are denominated "cascudos" in Brazil. Cytogenetic data about three "cascudos" species fished in the rio Araguaia are described in the present study. The Pterygoplichthys joselimaianus showed 2n=52, with 28 metacentrics (M) chromosomes, 16 submetacentrics (SM) and 8 subtelocentrics/acrocentrics (ST/A) in both sexes. Hemiancistrus spinosissimus showed 2n=52, with karyotype formulae 26M+22SM+4ST, in both sexes. Hemiancistrus spilomma also showed 2n=52, but in this species a ZZ/ZW sex chromosome system (25M+21SM+6ST in females and 24M+22SM+6ST in males) was observed. The cells from H. spinosissimus and P. joselimaianus showed one chromosome pair bearing Ag-NORs, while in the H. spilomma three chromosome pairs bearing AgNORs were detected. The data showed in this work reveal particular chromosomal characteristics, important for a good recognition of both Hemincistrus species, and also show the importance of the insertion of cytogenetic data on taxonomic phylogenetic studies.Os sistemas de água doce neotropicais possuem um alto número de espécies de peixes Siluriformes, muitas das quais popularmente conhecidas como cascudos no Brasil. Dados citogenéticos sobre três espécies de cascudos, capturadas na bacia do rio Araguaia são descritos no presente trabalho. Pterygoplichthys joselimaianus possui 2n=52, com 28 cromossomos metacêntricos (M), 16 submetacêntricos (SM) e 8 subtelecêntricos/acrocêntricos (ST/A) em ambos os sexos. Hemiancistrus spinosissimus revelou 2n=52, com fórmula cariotípica 26M+22SM+4ST, nos dois sexos. H. spilomma também possui 2n=52, porém, nesta espécie foi observado um sistema de cromossomos sexuais do tipo ZZ/ZW (25M+21SM+6ST para fêmeas e 24M+22SM+6ST para machos). As células de H. spinosissimus e P. joselimaianus mostraram um par de cromossomos portadores de Ag-RONs. Em H. spilomma, três pares de cromossomos portadores de Ag-RONs foram observados. Os dados apresentados neste trabalho revelam características cromossômicas particulares, importantes para um bom reconhecimento de ambas as espécies de Hemiancistrus e mostram a importância da inserção dos dados citogenéticos nos estudos taxonômicos e filogenéticos.

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Section: Methodsmentioning

confidence: 99%

Karyotype description of three species of Loricariidae (Siluriformes) and occurrence of the ZZ/ZW sexual system in Hemiancistrus spilomma Cardoso & Lucinda, 2003

2006

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“…Chromosome spreads were obtained by direct preparations from kidney cells (Bertollo et al, 1978) and by Heredity short-term culture of cells (Fenocchio et al, 1991), using stimulation of mitotic activity (Lee and Elder, 1980).…”

Section: Methodsmentioning

confidence: 99%

Evolutionary aspects of the ZZ/ZW sex chromosome system in the Characidae fish, genus Triportheus. A monophyletic state and NOR location on the W chromosome

Artoni

Bertollo

2002

Heredity

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Four species/populations of Triportheus, T. guentheri, T. cf. elongatus and T. paranense from different Brazilian hydrographic basins, were studied cytogenetically. All the species showed a similar karyotypic macrostructure, with a diploid chromosome number 2n = 52 and a ZZ/ZW sex chromosome system. Besides silver-and fluorochrome-staining, the chromosome mapping of 18S rDNA was also investigated using a biotinylated probe. In spite of some variation in the number of the NORs, a major chromosome site was always present on the short arm of an autosomal pair. In addition, a

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“…Mitotic chromosomes were obtained from short-term cell kidney cultures, as described elsewhere for fish cytogenetics (Fenocchio et al, 1991). Air-dried chromosome slides were stained with 5% Giemsa in phosphate buffer (pH 6.8).…”

Section: Chromosomal Preparationsmentioning

confidence: 99%

Genetic damage induced by trophic doses of lead in the neotropical fish Hoplias malabaricus (Characiformes, Erythrinidae) as revealed by the comet assay and chromosomal aberrations

et al. 2004

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The effects of clastogenic or mutagenic agents have rarely been studied in neotropical fish species exposed to contaminated water. In this study, the genetic damage caused by lead in the widely distributed South American fish, Hoplias malabaricus, was assessed using the comet (SCGE) assay and by testing for chromosomal aberrations. Eighteen specimens were acclimatized to laboratory conditions and then chronically exposed to contaminated food by feeding prey (Cyprinus sp.) injected intraperitoneally with doses of inorganic lead adjusted to give a contamination level of 21 µg of Pb 2+ .g -1 net weight of H. malabaricus. Three fish were sampled for chromosomal analysis after four doses (18 days) and another three after eight doses (41 days) of lead and the results then compared with three untreated controls kept under lead-free conditions. An additional six treated fish and three controls were sampled for the comet assay after 13 doses (64 days). Exposure to lead significantly increased the frequency of chromosomal aberrations and the frequency of tailed cell nuclei, the latter indicating DNA damage. These results show that H. malabaricus is a useful biological model for screening the clastogenic effects of lead and possibly other xenobiotics. The genetic damage seen here illustrates the need to investigate the potential effects of heavy metals on fish species in South America.

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Short term culture from solid tissues of fishes

Cited by 67 publications

References 13 publications

Karyotype description of three species of Loricariidae (Siluriformes) and occurrence of the ZZ/ZW sexual system in Hemiancistrus spilomma Cardoso & Lucinda, 2003

Karyotype description of three species of Loricariidae (Siluriformes) and occurrence of the ZZ/ZW sexual system in Hemiancistrus spilomma Cardoso & Lucinda, 2003

Evolutionary aspects of the ZZ/ZW sex chromosome system in the Characidae fish, genus Triportheus. A monophyletic state and NOR location on the W chromosome

Genetic damage induced by trophic doses of lead in the neotropical fish Hoplias malabaricus (Characiformes, Erythrinidae) as revealed by the comet assay and chromosomal aberrations

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