Short term culture of breast cancer tissues to study the activity of the anticancer drug taxol in an intact tumor environment

Kuip, Heiko van der; Mürdter, Thomas E.; Sonnenberg, Maike; McClellan, Monika; Gutzeit, Susanne; Gerteis, A; Simon, Wolfgang; Fritz, Péter; Aulitzky, Walter E.

doi:10.1186/1471-2407-6-86

Cited by 95 publications

(99 citation statements)

References 25 publications

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“…By using primary tumors in ex vivo culture, relatively unbiased analyses of the type of disease that is treated in the clinic can be evaluated. In keeping with other similar approaches, 18 the cultured tissue explants retained the associated stromal components that are known to play important roles in the pathogenesis and therapeutic sensitivities of cancer.…”

Section: Discussionmentioning

confidence: 89%

Therapeutic response to CDK4/6 inhibition in breast cancer defined by ex vivo analyses of human tumors

et al. 2012

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Section: Discussionmentioning

confidence: 89%

Therapeutic response to CDK4/6 inhibition in breast cancer defined by ex vivo analyses of human tumors

et al. 2012

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“…Tissue from large (>3 cm) and untreated mammary carcinoma was directly received after surgery. Cylindrical pieces with a radius of 5 mm were punched out and 0.2-mm-thick slices were cut as described before (21). Another part of the tumor tissue was dissociated during 1.5 h of incubation in an enzyme cocktail consisting of a protease, a DNase, and a collagenase (Sigma) at 37°C.…”

Section: Methodsmentioning

confidence: 99%

“…Large and untreated mammary carcinoma were directly received after surgery and cut into 0.2-mm-thick slices (21). Another part of the tumor tissue was dissociated, and EpCAM + and CD3 + cells were isolated.…”

Section: Cd4mentioning

confidence: 99%

Antiestrogens Induce Transforming Growth Factor β–Mediated Immunosuppression in Breast Cancer

et al. 2010

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Antiestrogens are universally used to treat estrogen receptor-positive breast cancer, but relapses occur commonly due to the development of drug resistance. The ability of antiestrogen to induce transforming growth factor β (TGFβ) in breast cancer cells may be relevant to the emergence of resistance, not only at the level of cell autonomous effects of TGFβ on cancer progression but also at the level of its effects on the host immune system. To evaluate the potential role of tumor-derived, antiestrogen-induced TGFβ as an immune suppressor, we established in vitro mixed lymphocyte tumor reactions (MLTR) using MCF-7 cells and peripheral blood mononuclear cells (PBMC), as well as tumor tissue and autologous tumor infiltrating lymphocytes (TIL) obtained from primary breast cancer biopsies. In allogeneic MLTR, antiestrogentreated MCF-7 cells caused downregulation of the effector molecules granzyme B, perforin, and Fas ligand in CD8 + T cells, and suppressed the generation of cytotoxic effector cells in a TGFβ-dependent manner.Furthermore, we documented induction of regulatory T cells in CD4 + T cells, based on Foxp3 expression and T-cell activation in cocultures. In autologous MLTR, antiestrogen treatment gave rise to enhanced Foxp3 expression of TIL/PBMC and decreased the number of apoptotic tumor cells. These effects were reversed by addition of a TGFβ neutralizing antibody. Our findings offer evidence that antiestrogen induces immunosuppression in the tumor microenvironment, through a TGFβ-dependent mechanism that may contribute to the development of antiestrogen resistance in breast cancer. Cancer Res; 70(4); 1314-22.

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“…Moreover, the system provides quick access to drug efficacy and presents relevant information to identify potential response as anticancer medicine in the complex microenvironment of tumor tissue (Van der Kuip et al 2006, Mitra et al 2013.…”

Section: Discussionmentioning

confidence: 99%

Immunocytochemical characterization of primary cell culture in canine transmissible venereal tumor

et al. 2016

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RESUMO.-[Caracterização imunocitoquímica de culturas primárias de tumor venéreo transmissível canino.]Os anticorpos anti-vimentina, anti-lisozima, anti-alfa 1 antitripsina, anti-CD3 e anti-CD79α foram empregados para a caracterização de culturas primárias de tumor venéreo transmissível canino (TVT). Amostras para cultura primá-ria e imuno-histoquímica foram coletadas de oito cães com diagnóstico clínico e citológico de TVT. Para validar o resultado inmunocitoquímico nas culturas de TVT foi realizada a contagem de cromossomos. Para a análise estatística o teste de Mann-Whitney foi empregado a um nível de significância de p<0.05. As culturas e os tecidos de TVT apresentaram intensa reatividade para vimentina, moderada a leve para Lisozima, moderada para alfa-antitripsina e não houve marcação para CD3 e CD79α. Immunochemistry with anti-vimentin, anti-lysozyme, anti-alpha 1 antitrypsin, anti-CD3 and anti-CD79α antibodies has been used for characterization of primary cell culture in the transmissible venereal tumor (TVT). Samples for primary cell culture and immunohistochemistry assays were taken from eight dogs with cytological and clinical diagnosis of TVT. To validate the immunochemical results in the primary cell culture of TVT, a chromosome count was performed. For the statistical analysis, the Mann-Whitney test with p<0.05 was used. TVT tissues and culture cells showed intense anti-vimentin immunoreactivity, lightly to moderate immunoreactivity for anti-lysozyme, and mild for anti-alpha-antitrypsin. No marking was achieved for CD3 and CD79α. All culture cells showed chromosomes variable number of 56 to 68. This is the first report on the use of immunocytochemical characterization in cell culture of TVT. Significant statistic difference between immunochemistry in tissue and culture cell was not established, what suggests that the use of this technique may provide greater certainty for the confirmation of tumors in the primary culture. This fact is particularly important because in vitro culture of tumor tissues has been increasingly used to provide quick access to drug efficacy and presents relevant information to identify potential response to anticancer medicine; so it is possible to understand the behavior of the tumor. Immunocytochemical characterization of primary cell culture in transmissible venereal tumor o uso da immunocitoquímica para a caracterização de culturas de TVT. Assim, e devido ao fato de se observar semelhança entre a imunomarcação em células e tecidos, sugere-se que o uso desta técnica possa auxiliar na confirmação de culturas primárias do tumor, fato muito importante porque a utilização da cultura do tumor pode permitir o acesso a informação relevante sobre resposta potencial a um tratamento e conhecimento do comportamento biológico do tumor.TERMOS DE INDEXAÇÃO: Tumor venéreo transmissível canino, alfa 1 antitripsina, citogenética, lisozima, vimentina.

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Short term culture of breast cancer tissues to study the activity of the anticancer drug taxol in an intact tumor environment

Cited by 95 publications

References 25 publications

Therapeutic response to CDK4/6 inhibition in breast cancer defined by ex vivo analyses of human tumors

Therapeutic response to CDK4/6 inhibition in breast cancer defined by ex vivo analyses of human tumors

Antiestrogens Induce Transforming Growth Factor β–Mediated Immunosuppression in Breast Cancer

Immunocytochemical characterization of primary cell culture in canine transmissible venereal tumor

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