Short-Term Feeding of Fish Oil Down-Regulates the Expression of Pyruvate Dehydrogenase E1 Alpha Subunit mRNA in Mouse Brain

Abstract

Previous studies have suggested that docosahexaenoic acid (DHA), contained in fish oil, prevents brain disease. In the current study, the effect of fish oil feeding on gene expression in the brain was investigated by suppression subtractive hybridization. We found that pyruvate dehydrogenase E1 alpha (PDHE1) mRNA expression is down-regulated by fish oil feeding. We examined whether the expression of PDHE1 mRNA is altered by DHA treatment in differentiated PC12 cells. PDHE1 mRNA was reduced by supplementation of DHA with a significant decrease in cellular ATP level. These results indicate that fish oil feeding might modulate energy metabolism in the brain.

Fish Oil Feeding Up-Regulates the Expression of 5-Aminolevulinate Synthase 2 mRNA in Rat Brain

“…They (183:2 AE 1:2 g) were divided into four groups of 10, and each group was fed a diet containing either 5% corn oil (LC), 20% corn oil (HC), 20% beef-tallow (HB), or 20% fish oil (HF). The full compositions of the diets were described previously, 9) and the change in the dietary level of fat was adjusted by replacing corn starch. The feeding experimental period was 35 d. Food intake and gain in body weight did not differ among the four groups.…”