Short-term liquid nitrogen storage of wild tomato (Solanum lycopersicum Mill.) seeds modifies the levels of phenolics in 7 day-old seedlings

Cryopreservation and Acclimatization of <i>Lycopersicon esculentum</i> (Mill.) Genotypes

“…1b, d). Cryopreservation of tomato has been reported for wild tomato seeds (60% regrowth) (Zevallos et al, 2013(Zevallos et al, , 2014, shoot meristems (52% regeneration) (Grout et al, 1978), andpollen (Karipidis andDouma, 2011). Length of roots (cm ± SD)* 0 16.6 ± 1.0 f 1.3 ± 0.5 d 1.4 ± 0.4 c 0.5 58.3 ± 1.7 c 7.6 ± 2.0 a 3.0 ± 0.8 b 1.0 77.7 ± 1.5 a 4.3 ± 2.5 c 4.7 ± 0.8 b 'Capriciu' 1.5…”

“…is one of the most extensively studied species not only for their importance as crop species but also as model system for genetic, molecular or physiological analyses (Gubiš et al, 2004). Various aspects of in vitro grown tomato cultures have been studied including micropropagation, selection of cell lines resistant to biotic / abiotic stress and production of haploids (Bhatia et al, 2004) or cryopreservation (Zevallos et al, 2013). Breeding efforts have raised the need for the conservation of valuable tomato germplasm (Grout and Crisp, 1995).…”

Cryopreservation of embryogenic tissues from mature holm oak trees

“…After 6 years in LN, just one third of them recovered growth, and only two were able to differentiate embryos [24]. In tomato, modifications in the phenolic content were observed after different periods of days in LN, although neither germination nor phenotypic variations were recorded [62]. Therefore, although ultralow temperatures arrest metabolic processes, some changes may happen with time in storage that caused the observed loss of differentiation ability.…”

Genetic integrity assessment of cryopreserved tomato(Lycopersicon esculentum Mill.) genotypes

“…Cryopreservation studies have been reported for tomato seeds (Stanwood, 1985;Zevallos et al, 2013;, shoot tips (Coste et al, 2014), meristems (Grout et al, 1978), and pollen (Sacks and St Clair, 1996;Karipidis and Douma, 2011). Our research showed that tomato shoot tips excised from in vitro-grown plants can be cryopreserved using the droplet vitrification protocol with a maximum of 70% shoot recovery rate in Pontica.…”

“…orthodox seeds, and one subsample of any accession should be systematically stored in liquid nitrogen, in addition to the samples stored under gene bank conditions (Engelmann and Ramanatha, 2012;Zevallos et al, 2013). Special consideration should be given to cryostorage of elite landraces, commercial varieties, and cultivars.…”