Short-Term Malnutrition in Neonatal Rabbits: Effect on Gastrointestinal Epithelial Proliferation

Baker, Susan S.; Campbell, Cara L.

doi:10.1093/jn/119.5.785

Cited by 4 publications

(2 citation statements)

References 15 publications

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“…However, in an autoradiographic study, cell migration showed a significant reduction in starved rats (12). Both decreased cell migration rate and DNA synthesis have only been described in the intestine of fasted neonatal rabbits, which also showed some regional differences (23).…”

Section: Discussionmentioning

confidence: 95%

Feeding manipulation elicits different proliferative responses in the gastrointestinal tract of suckling and weanling rats

Palanch

Alvares

1998

Braz J Med Biol Res

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Food deprivation has been found to stimulate cell proliferation in the gastric mucosa of suckling rats, whereas the weanling period has been reported to be unresponsive in terms of proliferative activity. In the present study we analyze regional differences in the effect of milk or food deprivation on cell proliferation of the epithelia of the esophagus and of five segments of small intestine in suckling, weanling and newly weaned Wistar rats of both sexes. DNA synthesis was determined using tritiated thymidine to obtain labeling indices (LI); crypt depth and villus height were also determined. Milk deprivation decreased LI by 50% in the esophagus (from 15 to 8.35%) and small intestine (from 40 to 20%) of 14-day-old rats. In 18-day-old rats, milk and food deprivation decreased LI in the esophagus (from 13 to 5%) and in the distal segments of the small intestine (from 36-40 to 24-32%). In contrast, the LI of the epithelia of the esophagus (5%) and of all small intestine segments (around 30%) of 22-day-old rats were not modified by food deprivation. Crypt depth did not change after treatment (80 to 120 µm in 14-and 22-day-old rats, respectively). Villus height decreased in some small intestine segments of unfed 14-(from 400 to 300 µm) and 18-day-old rats (from 480 to 360 µm). The results show that, contrary to the stomach response, milk deprivation inhibited cell proliferation in the esophagus and small intestine of suckling rats, demonstrating the regional variability of each segment of the gastrointestinal tract in suckling rats. In newly weaned rats, food deprivation did not alter the proliferation of these epithelia, similarly to the stomach, indicating that weanling is a period marked by the insensitivity of gastrointestinal epithelia to dietary alterations.

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Section: Discussionmentioning

confidence: 95%

Feeding manipulation elicits different proliferative responses in the gastrointestinal tract of suckling and weanling rats

Palanch

Alvares

1998

Braz J Med Biol Res

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“…Decreased TO 2 in the jejunum caused by feed deprivation for 48 h was associated with decreased Na + , K + -ATPase-dependent O 2 uptake. Changes in ouabaininsensitive respiration (OISO 2 ; Table 2) may be related to reduced protein synthesis for cell proliferation and for hydrolases related to digestion (Baker and Campbell 1989).…”

Section: Methodsmentioning

confidence: 99%

The effect of feed deprivation on gastrointestinal respiration and Na⁺, K⁺-ATPase in chicks

Park¹,

McBride²,

Milligan³

et al. 1998

Can. J. Anim. Sci.

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-Radford, L. M. 1998. The effect of feed deprivation on gastrointestinal respiration and Na + , K + -ATPase in chicks. Can. J. Anim. Sci. 78: 315-326. In order to investigate the effect of nutrient availability on energy metabolism of the gastrointestinal tract (GIT) of chicks, two experiments were conducted. In exp. 1, jejunal slices from male White Leghorn chicks at 14 d were incubated in salt-based balanced medium (SBM) or complex medium (CM) for 30 min. The total O 2 (TO 2 ) and ouabain-sensitive O 2 (OSO 2 ) consumption in jejunal slices were significantly decreased in SBM. The results from exp. 1 imply that when the GIT mucosa is subjected to nutrient deprivation, the utilization of oxygen consumption is altered primarily by shifting active Na+ pump activity. Changes in the TO 2 , OSO 2 and amiloride-sensitive O 2 (ASO 2 ) uptake in response to feed deprivation in the small intestine of male White Leghorn chicks were investigated in exp. 2. There was little change in TO 2 , OSO 2 and ASO 2 per unit of dry matter of the small intestine from birds subjected to fasting, except for a TO 2 decrease in the jejunum of 48 h fasted chicks. However, there were significant reductions in TO 2 , OSO 2 and ASO 2 in the jejunum when data were expressed per unit DNA. Na + , K + -ATPase-dependent respiration represented 31-37% of TO 2 in the avian jejunoileum. Amiloride-sensitive respiration accounted for 10-18% of TO 2 in the jejunoileum. The maximal amount of 3 Houabain binding sites of jejunal enterocytes was significantly decreased in chicks fasted for 48 h (P < 0.05).Key words: Oxygen consumption, fasting, Na + , K + -ATPase, ouabain, jejunum, ileum, chicks Park, H., McBride, B. W., Milligan, L. P. et Trouten-Radford, L. M. 1998. Effet du jeûne sur la respiration gastro-intestinale et sur la Na+, K+-ATPase chez le poulet. Can. J. Anim. Sci. 78: 315-326. Deux expériences ont été réalisées pour examiner l'effet de l'apport d'aliment sur le métabolisme énergétique du tractus gastro-intestinal (TGI) du poulet. Dans la première, des tranches du jéjunum de poulets White Leghorn âgés de 2 semaines étaient mises en incubation pendant 30 minutes dans un milieu salin équilibré (MSE) ou dans un milieu complexe (MC). La consommation de O 2 total (TO 2 ) et de O 2 sensible à la ouabaïne (OSO 2 ) était significativement abaissée dans le MSE. Il semble que, lorsque la muqueuse du TGI est exposée à une privation d'apports nutritifs, l'utilisation de la consommation de l'oxygène soit modifiée, surtout par une réorientation de l'activité de la pompe Na + . Dans la 2 e expérience, nous observions les modifications de l'absorption de TO 2 , de OSO 2 et de O 2 sensible à l'amiloride (ASO 2 ), résultant de la privation d'aliments, dans l'intestin grêle de poulets White Leghorn. On ne constatait que peu de changements de ces trois critères par unité de matière sèche d'intestin chez les sujets mis au jeûne, à part une baisse de TO 2 dans le jéjunum des poulets privés d'aliments pendant 48 h. Toutefois, lorsque les données étaient exprimées p...

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Stage of Development and Fasting Affect Protein Synthetic Activity in the Gastrointestinal Tissues of Suckling Rats

Burrin

Davis

Fiorotto

et al. 1991

The Journal of Nutrition

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We studied suckling rats from 1 to 28 d of age to determine the normal developmental changes in the protein synthetic activity of gastrointestinal tissue. We also studied the changes that occurred in response to 10 or 18 h of fasting at 5, 10, 16 and 28 d of age. Protein synthesis was measured in vivo using a flooding dose of L-[4-3H]phenylalanine. Fractional protein synthesis rates (FSR, %/d) of stomach and small intestines increased nearly 80% during the period normally associated with weaning (d 18 to 28). Between birth and 28 d, the pancreas FSR increased approximately 6.5-fold from 46 to 302%/d. The increases in stomach, small intestinal and pancreatic FSR were largely due to increases in translational efficiency (gram protein synthesized per gram RNA). At 5, 10, 16 and 28 d postpartum, both the FSR and translational efficiency in pancreatic and small intestinal tissues were decreased after 10 h of fasting; however, measures in stomach tissue were largely unaffected. The magnitude of decline in FSR and translational efficiency in response to fasting was greater during the earlier than in the later stages of development. The results suggest that the FSR in the pancreas, small intestines and stomach of suckling rats increase during the period of development associated with weaning. Fasting affected protein synthetic activity more profoundly in pancreatic and small intestinal tissue in the early stages of development.

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Short-Term Malnutrition in Neonatal Rabbits: Effect on Gastrointestinal Epithelial Proliferation

Cited by 4 publications

References 15 publications

Feeding manipulation elicits different proliferative responses in the gastrointestinal tract of suckling and weanling rats

Feeding manipulation elicits different proliferative responses in the gastrointestinal tract of suckling and weanling rats

The effect of feed deprivation on gastrointestinal respiration and Na⁺, K⁺-ATPase in chicks

Stage of Development and Fasting Affect Protein Synthetic Activity in the Gastrointestinal Tissues of Suckling Rats

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Short-Term Malnutrition in Neonatal Rabbits: Effect on Gastrointestinal Epithelial Proliferation

Cited by 4 publications

References 15 publications

Feeding manipulation elicits different proliferative responses in the gastrointestinal tract of suckling and weanling rats

Feeding manipulation elicits different proliferative responses in the gastrointestinal tract of suckling and weanling rats

The effect of feed deprivation on gastrointestinal respiration and Na+, K+-ATPase in chicks

Stage of Development and Fasting Affect Protein Synthetic Activity in the Gastrointestinal Tissues of Suckling Rats

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The effect of feed deprivation on gastrointestinal respiration and Na⁺, K⁺-ATPase in chicks