Short-term Quinolones for Successful Eradication of Multiply Resistant Vibrio Cholerae in Adult Patients

Doğancı, Levent; Gün, Hüseyin; Baysallar, Mehmet; Albay, Ali; Çinar, Eşref; Haznedaroğlu, Tunçer

doi:10.3109/00365549509032747

Cited by 7 publications

(4 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Changes in susceptibility pattern have been attributed to isolation time, source and geographical location. However, high activities of ofloxacin and ciprofloxacin observed in our study are similar to other studies [15, 38, 39] and corroborate the use of fluoroquinolones as new alternatives for cholera treatment in some cholera endemic regions [40]. Our data and reports from other cholera endemic localities in Douala [20] therefore suggest ciprofloxacin and ofloxacin could be very good alternatives for chemotherapy during cholera or cholera-like disease outbreaks in Douala.…”

Section: Discussionsupporting

confidence: 92%

Water sources as reservoirs of Vibrio choleraeO1 and non-O1 strains in Bepanda, Douala (Cameroon): relationship between isolation and physico-chemical factors

Akoachere

Mbuntcha

2014

BMC Infect Dis

View full text Add to dashboard Cite

BackgroundCholera has been endemic in Douala since 1971. Most outbreaks start from Bepanda, an overcrowded neighbourhood with poor hygiene and sanitary conditions. We investigated water sources in Bepanda as reservoirs of Vibrio cholerae, the causative agent of cholera, determined its antibiotic susceptibility and some physico-chemical characteristics that could maintain the endemicity of this organism in Bepanda.MethodsThree hundred and eighteen water samples collected from 45 wells, 8 taps and 1 stream from February to July 2009 were analyzed for V. cholerae using standard methods. Isolates were characterized morphologically, biochemically and serologically. The disc diffusion technique was employed to investigate antibiotic susceptibility. Differences in prevalence of organism between seasons were analysed. Correlation strength and direction of association between physico-chemical parameters and occurrence of V. cholerae was analyzed using the Kendall tau_b non-parametric correlation. This was further confirmed with the forward-stepwise binary logistic regression.ResultsEighty-seven (27.4%) samples were positive for V. cholerae. Isolation was highest from wells. The organism was isolated in the rainy season and dry season but the frequency of isolation was significantly higher (χ2 = 7.009, df = 1, P = 0.008) in the rainy season. Of the 96 confirmed V. cholerae isolates, 32 (33.3%) belonged to serogroup O1 and 64 (66.6%) were serogroup non-O1/non-O139. Isolates from tap (municipal water) were non-O1/non-O139 strains. Salinity had a significant positive correlation with isolation in the dry season (+0.267, P = 0.015) and rainy season (+0.223, P = 0.028). The forward-stepwise method of binary logistic regression indicated that as pH (Wald = 11.753, df = 1), P = 0.001) increased, odds of isolation of V. cholerae also increased (B = 1.297, S.E = 0.378, Exp(B) = 3.657). All isolates were sensitive to ciprofloxacin and ofloxacin. Multi-drug resistance was predominant among the non-O1/non-O139 isolates.ConclusionV. cholerae was found in wells and stream in both seasons. Cholera will continue to be a health threat in Bepanda if intervention measures to prevent outbreak are not implemented. Continuous monitoring of water sources in this and other cholera high-risk areas in Cameroon is necessary, for a better preparedness and control of cholera.Electronic supplementary materialThe online version of this article (doi:10.1186/1471-2334-14-421) contains supplementary material, which is available to authorized users.

show abstract

Section: Discussionsupporting

confidence: 92%

Water sources as reservoirs of Vibrio choleraeO1 and non-O1 strains in Bepanda, Douala (Cameroon): relationship between isolation and physico-chemical factors

Akoachere

Mbuntcha

2014

BMC Infect Dis

View full text Add to dashboard Cite

show abstract

“…Almost all O1 and non-O1 strains displayed susceptibility to the broad-spectrum fluoroquinolones, such as ciprofloxacin and ofloxacin. It is important to note, however, that two clinical O1 strains showed intermediate resistance to these drugs while clinical studies have shown that these drugs are effective in the treatment of cholera [31,32], the emergence of fluoroquinolone-resistant strains of V. cholerae O1 has been reported [28]. The results from this study suggest that the treatment of diarrhoeal patients with alternative drugs such as fluoroquinolones will probably become problematic in the future due to the emergence of resistance.…”

Section: Analysis Of Antibiotic Resistancementioning

confidence: 74%

Genetic diversity and antibiotic resistance of clinical and environmental Vibrio cholerae suggests that many serogroups are reservoirs of resistance

Campos

Zahner²,

Avelar³

et al. 2004

Epidemiol. Infect.

View full text Add to dashboard Cite

Vibrio cholerae is an important human pathogen and the cause of cholera. Since genetic variation and antibiotic resistance of strains have implications for effective treatment of the disease, we examined the genetic diversity and antibiotic resistance profile in 92 clinical strains (serogroup O1) and 56 environmental strains (O1 antigen, 42 strains; non-O1 antigen, 14 strains) isolated in Brazil between 1991 and 1999. Clinical and environmental O1 strains showed greater drug resistance compared to environmental non-O1 strains. Nearly all clinical O1 strains were resistant to one or more antibiotics while half of the environmental O1 and non-O1 strains were resistant to one or more antibiotics. No plasmids or class 1 integrons were detected in the strains by PCR analysis. Multilocus enzyme electrophoresis analysis (MLEE) suggests most of the O1 strains belong to a single (South American) clone that is related but different to seventh-pandemic strains isolated from other parts of the world. Our results show that there is a close genetic relationship between clinical and environmental O1 strains and that many serogroups and the environment can be a reservoir for antibiotic resistance.

show abstract

“…The antibiotics that were effective with all the studied strains were ciprofloxacin and doxycycline, which connected with other antibiotics as the fluoroquinolones are being evaluated nowadays to determine new alternatives in the treatment against the cholera but whose use is uncommon in our country [29][30][31]. In developed countries, these antibiotics would be probably the election drugs because they quickly reduce the fecal excretion of Vibrio, and they can cover a wide spectrum of enteropathogens including V. cholera.…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial resistance of clinical and environmental strains of Vibrio cholerae isolated in Lima-Peru during epidemics of 1991 and 1998

Ibarra¹,

Alvarado²

2007

Braz J Infect Dis

View full text Add to dashboard Cite

Short-term Quinolones for Successful Eradication of Multiply Resistant Vibrio Cholerae in Adult Patients

Cited by 7 publications

References 5 publications

Water sources as reservoirs of Vibrio choleraeO1 and non-O1 strains in Bepanda, Douala (Cameroon): relationship between isolation and physico-chemical factors

Water sources as reservoirs of Vibrio choleraeO1 and non-O1 strains in Bepanda, Douala (Cameroon): relationship between isolation and physico-chemical factors

Genetic diversity and antibiotic resistance of clinical and environmental Vibrio cholerae suggests that many serogroups are reservoirs of resistance

Antimicrobial resistance of clinical and environmental strains of Vibrio cholerae isolated in Lima-Peru during epidemics of 1991 and 1998

Contact Info

Product

Resources

About