Short-term Resistance Training Increases APPL1 Content in the Liver and the Insulin Sensitivity of Mice Fed a Long-term High-fat Diet

Minuzzi, Luciéle Guerra; Kuga, Gabriel Keine; Breda, Leonardo; Gaspar, Rafael Calais; Muñoz, Vitor Rosetto; Pereira, Rodrigo Fernando; Botezelli, José Diego; Silva, Adelino Sánchez Ramos da; Cintra, Dennys Esper; Moura, Leandro Pereira de; Ropelle, Eduardo Rochete; Pauli, José Rodrigo

doi:10.1055/a-0885-9872

Cited by 9 publications

(7 citation statements)

References 35 publications

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“…Also, Domingos et al, (2012) reported activation of the AMPK pathway and stimulation of lipid oxidation in the liver following resistance training, which decreased lipogenic enzymes such as SREPB-1c, ACC, SCD-1, and fatty acid synthase (19). Resistance training improves IR in rats fed a high-fat diet (28). It appears that in our study, adding sucrose solution along with a high-fat diet had a significant effect on IR, and PRT could not have a significant effect on IR and consequently the expression of these enzymes.…”

Section: Discussionmentioning

confidence: 98%

Investigating the Levels of Liver Lipogenic and Lipolytic Enzymes in Rat with High-Fat Diet and Sucrose Solution Underwent Progressive Resistance Training

delbari

Fathi

Safarzade

et al. 2023

IJDO

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Objective: Consuming too much fat or carbohydrates stimulates lipogenesis and excess fat is stored in non-fat tissues, including the liver, and manifests as obesity and fatty liver disease. This study aimed to investigate the effect of eight weeks of progressive resistance training (PRT) on the liver levels of some enzymes affecting lipid metabolism in rats fed a high-fat diet and sucrose solution. Materials and Methods: Twenty-four male wistar rats with 5 weeks of age were randomly divided into two groups: standard diet (SD) (n=8) and high-fat diet and sucrose solution (HFDS) (n=16). Twelve weeks later, HFDS group was divided into two groups: sedentary (HS) and PRT (HPRT). The PRT program was implemented 3 days a week for 8 weeks. Gene expression of AMPKα1, SCD-1, ATGL and FASN enzymes affecting lipid metabolism in liver tissue and its fat content were investigated. Results: HFDS significantly increased the body weight (P: 0.001) and significantly decreased the liver expression of ATGL and FASN (P: 0.001, P: 0.011). Eight weeks of PRT did not show a significant difference in the expression of AMPKα1, SCD-1, ATGL and FASN genes. Rats fed HFDS had considerably higher levels of triglyceride (TG) and total cholesterol (TC) in their liver tissue (P: 0.004, P: 0.001) and PRT did not affect them (P: 0.959, P: 0.809 respectively). Conclusion: It seems that eight weeks of PRT will not change liver lipid metabolism enzymes. Therefore, modifying the diet and changing it, will probably show different results after PRT.

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Section: Discussionmentioning

confidence: 98%

Investigating the Levels of Liver Lipogenic and Lipolytic Enzymes in Rat with High-Fat Diet and Sucrose Solution Underwent Progressive Resistance Training

delbari

Fathi

Safarzade

et al. 2023

IJDO

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“…The results show that short-term RT increased the APPL1 content, which was accompanied by an improvement in insulin sensitivity. 37 To investigate the effects of IKKϵ deletion, Chiang and colleagues 38 observed an increased energy expenditure and protection from the negative impacts of a high-fat diet in knockout (KO) IKKϵ mice, suggesting a relation of IKKϵ in the thermogenesis pathway, such as uncoupling protein 1 (UCP1) expression. Additionally, in the liver, it was shown that IKKϵ content was elevated after high-fat feeding.…”

Section: Discussionmentioning

confidence: 99%

“…Data report that hepatic Akt and IRS1 phosphorylation was higher in the exercised group than in the obese sedentary by reducing protein‐tyrosine phosphatase 1B (PTP1B) 36 . Minuzzi et al 37 also investigated the effects of short‐term resistance training (RT) on APPL1 content and adiponectin pathway in the liver of mice fed a long‐term high‐fat diet. The results show that short‐term RT increased the APPL1 content, which was accompanied by an improvement in insulin sensitivity 37 …”

Section: Discussionmentioning

confidence: 99%

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Resistance exercise attenuates IKKε phosphorylation and hepatic fat accumulation of obese mice

Antunes

Lima

Vieira

et al. 2022

Clin Exp Pharma Physio

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Obesity is associated with low-grade inflammation and disturbances in hepatic metabolism. This study aimed to investigate the effects of resistance exercise on inflammatory signalling related to IκB kinase (IKK) ϵ protein (IKKϵ) and on hepatic fat accumulation in obese mice. Male Swiss mice were distributed into three groups: control (CTL) fed with standard chow; obese (OB) mice induced by a high-fat diet (HFD); obese exercised (OB + RE) mice fed with HFD and submitted to a resistance exercise training. The resistance exercise training protocol consisted of 20 sets/3 ladder climbs for 8 weeks, three times/week on alternate days. The training overload was equivalent to 70% of the maximum load supported by the rodent. Assays were performed to evaluate weight gain, hepatic fat content, fasting glucose, insulin sensitivity, IKKϵ phosphorylation and proteins related to insulin signalling and lipogenesis in the liver. Mice that received the high-fat diet showed greater adiposity, impaired insulin sensitivity, increased fasting glucose and increased hepatic fat accumulation. These results were accompanied by an increase in IKKϵ phosphorylation and lipogenesis-related proteins such as cluster of differentiation 36 (CD36) and fatty acid synthase (FAS) in the liver of obese mice. In contrast, exercised mice showed lower body weight and adiposity evolution throughout the experiment. In addition, resistance exercise suppressed the effects of the high-fat diet by reducing IKKϵ phosphorylation and hepatic fat content. In conclusion, resistance exercise training improves hepatic fat metabolism and glycaemic homeostasis, which are, at least in part, linked to the anti-inflammatory effect of reduced IKKϵ phosphorylation in the liver of obese mice.

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“…RT was executed according to the protocol described by Minuzzi with several adjustments [22]. First, an empty weight-bearing device was immobilized on the tail while the mice were acclimated to the ladder.…”

Section: Maximum Voluntary Carrying Capacity (Mvcc) Test and Resistan...mentioning

confidence: 99%

Adaptation of Resistance Training is impaired in muscle-specific PGC-1α overexpressing mice

ZHANG,

SONG,

BAI

et al. 2024

Preprint

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Resistance training (RT) is important for skeletal muscle health. However, compared with RT implemented alone, concurrent training can attenuate RT adaptations. We verify the effects of RT on muscle-enriched or hypertrophy-related miRNAs, mammalian target of rapamycin complex 1 (mTORC1) anabolic signaling proteins, and oxidative metabolism-related proteins in mice with different endurance muscle Phenotype. In the present study, we found that muscle-specific overexpression of PGC-1alpha attenuated RT adaptations and resulted in different expression of miR-222-3p and miR-206-3p after RT. The expression level of miR-206-3p and miR-222-3p in Wt+ mice (wild-type trained mice) was significantly higher than that in Tg+ mice (MCK-PGC-1α transgenic trained mice). This suggested that discordant miR-222-3p and miR-206-3p expression levels after RT may play a role in the mechanism contributing to the interference effects. With regard to protein expression,there was no difference in mTORC1 between the genotypes. Furthermore, the levels of parameters for oxidative metabolism were significantly higher in Tg mice than in Wt mice. Our study indicated miR-222-3p and miR-206-3pas novel molecules that could provide a new research direction into the mechanisms underlying the concurrent training effect.

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Short-term Resistance Training Increases APPL1 Content in the Liver and the Insulin Sensitivity of Mice Fed a Long-term High-fat Diet

Cited by 9 publications

References 35 publications

Investigating the Levels of Liver Lipogenic and Lipolytic Enzymes in Rat with High-Fat Diet and Sucrose Solution Underwent Progressive Resistance Training

Investigating the Levels of Liver Lipogenic and Lipolytic Enzymes in Rat with High-Fat Diet and Sucrose Solution Underwent Progressive Resistance Training

Resistance exercise attenuates IKKε phosphorylation and hepatic fat accumulation of obese mice

Adaptation of Resistance Training is impaired in muscle-specific PGC-1α overexpressing mice

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