Short-term serum and urinary changes in sex hormones of healthy pre-pubertal children after the consumption of commercially available whole milk powder: a randomized, two-level, controlled-intervention trial in China

Wu, Jieshu; Shi, Xi; Zhang, Man; Lü, Xiaolong; Qin, Rui; Hu, Manli; Wang, Zhixu

doi:10.1039/d2fo02321k

Cited by 2 publications

(2 citation statements)

References 42 publications

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“…The simultaneous measurement of FSH, LH, PRL, and AMH can provide a comprehensive hormonal profile that is useful in assessing the stage of pubertal development, identifying underlying disorders, and differentiating between central and peripheral causes. , Therefore, the simultaneous assessment of the four reproductive hormone protein levels is of great importance for the detection of reproductive health. Furthermore, the change of urinary FSH, LH, and PRL levels also indicates the problems of reproductive health and demonstrates the efficacy of fertility treatments. , Nevertheless, recent studies have failed to detect the presence of AMH in urine. , Therefore, it is essential to conduct a thorough exploration into urinary AMH levels with the assistance of a more sensitive detection technique for studying the relationship between AMH levels and the development of related diseases.…”

Section: Introductionmentioning

confidence: 99%

“…Furthermore, the change of urinary FSH, LH, and PRL levels also indicates the problems of reproductive health and demonstrates the efficacy of fertility treatments. 48,49 Nevertheless, recent studies have failed to detect the presence of AMH in urine. 50,51 Therefore, it is essential to conduct a thorough exploration into urinary AMH levels with the assistance of a more sensitive detection technique for studying the relationship between AMH levels and the development of related diseases.…”

Section: ■ Introductionmentioning

confidence: 99%

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A Quadruplex Ultrasensitive Immunoassay for Simultaneous Assessment of Human Reproductive Hormone Proteins in Multiple Biofluid Samples

Cai,

Jin,

Zong

et al. 2023

Anal. Chem.

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Reproductive hormones play vital roles in reproductive health and can be used to assess a woman’s ovarian function and diagnose diseases associated with reproductive endocrine disorders. As these hormones are important biomarkers for reproductive health monitoring and diagnosis, a rapid, high-throughput, and low-invasive detection and simultaneous assessment of the levels of multiple reproductive hormones has important clinical applications. In this work, a quadruplex ultrasensitive immunoassay was developed for simultaneous assessment of 4 human reproductive hormone proteins (follicle-stimulating hormone (FSH), luteinizing hormone (LH), prolactin (PRL), and anti-Müllerian hormone (AMH)) in a variety of human biofluid samples. This assay takes advantage of single-molecule imaging of microwell arrays and capture antibody beads as a reaction interface to construct multiplex bead array immunoassays. The analyte-bound beads can easily be parsed to individual wells and detected via fluorophores, emitting distinct wavelengths associated to the beads. As a result, this proposed quadruplex immunoassay exhibits four good 4-parameter logistic calibration curves ranging from 2.7 to 2000, 1.6 to 1200, 1.8 to 1300, and 0.3 to 220 pg/mL with limits of detection of 0.32, 0.28, 0.14, and 0.02 pg/mL for FSH, LH, PRL, and AMH, respectively. Furthermore, the developed quadruplex immunoassay was used to test clinical venous serum samples where it showed remarkable consistency with clinical test results in methodological comparison and the diagnosis of polycystic ovary syndrome. In addition, we successfully applied the ultrasensitive capability of this assay to the simultaneous testing and evaluation of four proteins in fingertip blood as well as urine samples, in which the urinary AMH level (1.42–156 pg/mL) was measured and assessed quantitatively for the first time.

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Section: Introductionmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

A Quadruplex Ultrasensitive Immunoassay for Simultaneous Assessment of Human Reproductive Hormone Proteins in Multiple Biofluid Samples

Cai,

Jin,

Zong

et al. 2023

Anal. Chem.

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Dietary Progesterone Contributes to Intratissue Levels of Progesterone in Male Mice

et al. 2023

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Progesterone serum levels have been identified as a potential predictor for treatment effect in men with advanced prostate cancer, which is an androgen-driven disease. Although progesterone is the most abundant sex steroid in orchiectomized (ORX) male mice, the origins of progesterone in males are unclear. To determine the origins of progesterone and androgens, we first determined the effect of ORX, adrenalectomy (ADX), or both (ORX+ADX) on progesterone levels in multiple male mouse tissues. As expected, intra-tissue androgen levels were mainly testicular-derived. Interestingly, progesterone levels remained high after ORX and ORX+ADX with the highest levels in white adipose tissue (WAT) and in the gastrointestinal tract. High progesterone levels were observed in mouse chow and exceptionally high progesterone levels were observed in food items such as dairy, eggs, and beef, all derived from female animals of reproductive age. To determine if orally ingested progesterone contributes to tissue levels of progesterone in males, we treated ORX+ADX and sham mice with isotope labeled progesterone or vehicle by oral gavage. We observed a significant uptake of labeled progesterone in WAT and prostate, suggesting that dietary progesterone may contribute to tissue levels of progesterone. In conclusion, although adrenal-derived progesterone contributes to intra-tissue progesterone levels in males, non-adrenal progesterone sources also contribute. We propose that dietary progesterone is absorbed and contributes to intra-tissue progesterone levels in male mice. We speculate that food with high progesterone content could be a significant source of progesterone in males, possibly with consequences for men undergoing androgen deprivation therapy for prostate cancer.

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Short-term serum and urinary changes in sex hormones of healthy pre-pubertal children after the consumption of commercially available whole milk powder: a randomized, two-level, controlled-intervention trial in China

Abstract: Progesterone was found in commercial milk. After consuming this milk, compared with the control, serum progesterone levels after 3 h and urinary pregnanediol levels within 4 h increased, but those in urine after 48 h had no significant change.

Cited by 2 publications

References 42 publications

A Quadruplex Ultrasensitive Immunoassay for Simultaneous Assessment of Human Reproductive Hormone Proteins in Multiple Biofluid Samples

A Quadruplex Ultrasensitive Immunoassay for Simultaneous Assessment of Human Reproductive Hormone Proteins in Multiple Biofluid Samples

Dietary Progesterone Contributes to Intratissue Levels of Progesterone in Male Mice

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