SHORT-TERM STORAGE OF ALGINATE-ENCAPSULATED PROTOCORM-LIKE BODIES OF PHALAENOPSIS CORNU-CERVI (BREDA) BLUME &amp; RCHB. F.

Rittirat, Suphat; Klaocheed, S.; Thammasiri, K.

doi:10.17660/actahortic.2015.1078.9

Cited by 5 publications

(6 citation statements)

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“…This, in turn, can instigate an excessive accumulation of phenolic compounds, ultimately resulting in browning. The compounds were generated due to the reaction between oxygen and phenol oxidase enzymes to form toxic quinone compounds that cause cell death (Pirttilä et al, 2008;Rittirat et al, 2012;Ru et al, 2013).…”

Section: Discussion Discussion Discussionmentioning

confidence: 99%

“…According to Amente and Chimdessa (2021), the incidence can be reduced by incorporating anti-phenolic compounds into the medium. (Rittirat et al, 2012) successfully applied 0.2% activated charcoal (AC) for Phalaenopsis cornucervi (Breda) Blume and Rchb. f. and 0.2 % AC for Cymbidium aloifolium (Nongdam and Chongtham, 2011).…”

Section: Introduction Introduction Introduction Introductionmentioning

confidence: 99%

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Optimizing the potential utilization of bioreactors for the mass propagation of Indonesian Dendrobium varieties

RACHMAWATI,

PRAMANIK,

SHINTIAVIRA

et al. 2024

Not Bot Horti Agrobo

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The study focuses on two Indonesia Dendrobium varieties, D. Dian Agrihorti (DDA) and D. Syifa Agrihorti (DSA), which have potential to be commercialized, but face limitations on the availability of qualified seedlings sustainably. The research aimed to establish an in vitro propagation protocol using a Temporary Immersion System (TIS) to produce high-quality seedlings efficiently. Various factors, including varieties, media, plant growth regulators, anti-phenol compounds, organic additives, and TIS settings, were investigated using the basal part of the plantlet as the explant source. Key findings revealed that DDA outperformed DSA across all observed variables. In the initiation phase, basal plantlets cultured in Murashige and Skoog (MS) medium supplemented with 1.0 mg L-1 thidiazuron and 0.5 mg L-1 N-6 benzylaminopurine enhanced embryogenic callus (EC) formation, with a 13.5-day initiation period, 72% potential explant growth, 0.41 cm callus size, and a 3.45 rate of multiplication. During the proliferation stage, the addition of 150 mg L-1 ascorbic acid (AC) and the application of a TIS with a 30-minute dry period and a 15-minute wet period resulted in a 515.5% increase in EC fresh weight for DDA accompanied by a 6.16 multiplication rate. Regeneration of shoots was achieved using a Vacin and Went medium with 150 g L-1 banana extract, yielding 29.2 shoots per clump. Subsequent rooting of the shoots in 2 g L-1 Hyponex® medium with 20 g L-1 sugar and 2% AC proved successful. Acclimatization of plantlets with Cycas rumpii bulk demonstrated a 100% survivability rate. The established propagation protocol for DDA holds significant potential for application to other Dendrobium varieties, offering a sustainable and efficient method for meeting commercial demands in the Indonesian market.

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Section: Discussion Discussion Discussionmentioning

confidence: 99%

Section: Introduction Introduction Introduction Introductionmentioning

confidence: 99%

Optimizing the potential utilization of bioreactors for the mass propagation of Indonesian Dendrobium varieties

RACHMAWATI,

PRAMANIK,

SHINTIAVIRA

et al. 2024

Not Bot Horti Agrobo

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“…Pemanjangan akar tanaman terjadi karena proses pemuaian dan pemanjangan sel. Menurut Rittirat (2012) gula pasir sebagai sumber karbon yang berperan pengatur siklus sel yang berkaitan dengan tumbuhan.…”

Section: Panjang Akar (Cm)unclassified

PEMBERIAN GULA PASIR DAN PUPUK GAVIOTA MENGGUNAKAN TEKNIK PERENDAMAN TANAMAN ANGGREK BULAN (Phalaenopsis hibrida) PADA FASE PEMBIBITAN

agustina

Nuhayati

Sumarmi

2023

JIA

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Penelitian yang berjudul Pengaruh Pemberian Gula Pasir dan Pupuk Gaviota Menggunakan Teknik Perendaman Pada Tanaman Anggrek Bulan (phalaenopsis hibrida) Pada Fase Pembibitan dilaksanakan pada tanggal 17 Oktober 2022 sampai dengan tanggal 9 Januari 2023 di Kebun Green Leaves Orchid yang berada di Desa Sinoman Tempel, Kecamatan Sidorejo, Salatiga. Tujuan penelitian ini untuk mengetahui pengaruh pemberian Gula Pasir dan Pupuk Gaviota dengan dosis tertentu terhadap pertumbuhan tanaman anggrek bulan fase pembibitan. Penelitian menggunakan Rancangan Acak Lengkap (RAL) yang terdiri dari 2 faktor perlakuan dan 3 ulangan. Faktor pertama adalah pupuk Gaviota (P) dengan 3 taraf yaitu tanpa perlakuan 0 g/liter (P0), 15 g/liter) dan 25 g/liter (P2), faktor kedua yaitu Gula Pasir (S) dengan 3 taraf yaitu tanpa perlakuan 0 g/liter (S0), 2,5 g/liter (S1), 3,5 g/liter (S2). Parameter yang diamati meliputi jumlah akar, panjang akar (cm), jumlah daun baru, panjang daun (cm), lebar daun (cm), persentase tumbuh. Berdasarkan hasil penelitian, dan pembahasan maka dapat disimpulkan bahwa : kedua perlakuan memberikan respon atau berpengaruh terhadap perlakuan dosis pupuk Gaviota dan sukrosa. Kata Kunci : Anggrek Bulan, Pupuk Gaviota, Gula Pasir

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“…Pemberian perlakuan konsentrasi sukrosa sebesar 40 g/l menunjukkan nilai rata-rata jumlah akar 15,33 buah dibandingkan dengan perlakuan sukrosa 20 g/l dan 30 g/l yang menghasilkan rata-rata jumlah akar 4,2 dan 6,4 buah. Rittirat et al (2012) menyatakan bahwa penambahan sukrosa dengan konsentrasi 40 g/l merupakan konsentrasi paling optimum dalam menambah tinggi planlet, bobot basah, jumlah daun, jumlah akar dan panjang akar.…”

Section: Jumlah Akarunclassified

PENGARUH PENGGUNAAN SUKROSA DAN IBA TERHADAP INDUKSI AKAR EKSPLAN TUNAS ANGGREK (Dendrobium sp.) SECARA IN VITRO

Rustam

Rajani

2022

AGROTEK

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Dendrobium sp. is well known as the largest genus in orchid, every region has its own characteristics. The aim of the research was to examine the interaction effect of sucrose and IBA (indole 3-butyric acid) on root initiation of Dendrobium sp. in vitro culture. This study used a completely randomized design (CRD) factorial design 3x4 with 3 replications. The first factor was sucrose concentration (S) : S1 (20 g/l), S2 (30 g/l) and S3 (40 g/l). The second factor was IBA concentration (I) : I0 (0 mg/l), I1 (1 mg/l), I2 (2 mg/l) and I3 (3 mg/l). The parameters observed were number of roots, length of root, initiation root period, height of plantlets, number of shoots and culture success percentages. Data was analysed using analysis of variance and further test using LSD (least significance different) with a 5% level. The result showed that the concentration of sucrose 40 g/l had a significant effect on number of roots, length of root, height of plantlets and number of shoots. Concentrations of IBA 2 mg/l had a significant effect on height of plantlets. Iinteraction of both sucrose and IBA had no significant effect on all of the parameters. Two objects of culture were contaminated by fungi. Suggest for this study was to increase sucrose and IBA concentrations for the best result combination

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SHORT-TERM STORAGE OF ALGINATE-ENCAPSULATED PROTOCORM-LIKE BODIES OF PHALAENOPSIS CORNU-CERVI (BREDA) BLUME & RCHB. F.

Cited by 5 publications

References 0 publications

Optimizing the potential utilization of bioreactors for the mass propagation of Indonesian Dendrobium varieties

Optimizing the potential utilization of bioreactors for the mass propagation of Indonesian Dendrobium varieties

PEMBERIAN GULA PASIR DAN PUPUK GAVIOTA MENGGUNAKAN TEKNIK PERENDAMAN TANAMAN ANGGREK BULAN (Phalaenopsis hibrida) PADA FASE PEMBIBITAN

PENGARUH PENGGUNAAN SUKROSA DAN IBA TERHADAP INDUKSI AKAR EKSPLAN TUNAS ANGGREK (Dendrobium sp.) SECARA IN VITRO

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