Short term storage stability at room temperature of two different platelet-rich plasma preparations from equine donors and potential impact on growth factor concentrations

Abstract

BackgroundThe increasing interest in platelet-rich plasma (PRP) based therapies is as yet accompanied by inconsistent information regarding nearly all aspects of handling and application. Among these storage stability of processed platelet-rich products may be the basis for a more flexible application mode. The objective of this study was (1) to estimate the storage stability of growth factors platelet derived growth factor BB (PDGF-BB) and transforming growth factor ß1 (TGF-ß1) in both, a single-step softspin centrifugation-based pure-PRP (P-PRP, ACP®), and a gravity filtration system-based leukocyte-rich-PRP (L-PRP, E-PET), over a six hours time span after preparation at room temperature and (2) to identify possible factors influencing these growth factor concentrations in an equine model.ResultsGrowth factor concentrations remained stable over the entire investigation period in L-PRP as well as P-PRP preparations revealing a mean of 3569 pg/ml PDGF-BB for E-PET and means of 1276 pg/ml PDGF-BB and 5086 pg/ml TGF-ß1 for ACP®. Pearson correlations yielded no significant impact of whole blood platelet (PLT), white blood cell (WBC) and red blood cell (RBC) counts on resulting cytokine values. In case of ACP® no significant dependencies between PLT, WBC and RBC counts of the processed platelet-rich product and resulting cytokine content occurred with exception of TGF-ß1 concentrations showing a strong correlation with the WBC content. PDGF-BB content of E-PET preparations showed a strong positive correlation with PLT and a strong negative with WBC of these preparations but not with RBC.ConclusionsL-PRP ad modum E-PET and P-PRP ad modum ACP® are applicable over at least a six hours time span at room temperature without loss of growth factor content. Based on the results of this study factors influencing the resulting growth factor concentrations still remain questionable. Additional studies implicating a further standardization of preparation protocols are necessary to identify consistent impact on cytokine content after PRP processing.Electronic supplementary materialThe online version of this article (doi:10.1186/s12917-016-0920-4) contains supplementary material, which is available to authorized users.

The effect of four different freezing conditions and time in frozen storage on the concentration of commonly measured growth factors and enzymes in equine platelet-rich plasma over six months

“…All samples were placed in their respective storage conditions within 2 h of PRP preparation. Previous studies with equine and human blood have shown that concentrations of TGF-β1, PDGF-BB, and MMP-9 do not change within 4–6 h from the time of PRP preparation [26, 27]. The -20A was monitored using an Elitech RC-5 USB temperature data logger, to record temperature fluxes.…”

Effects of three blood derived products on equine corneal cells, an in vitro study

“…Furthermore, adaption time of corneal cells to PRP media might have been prolonged, due to the presence of additional cellular particles in PRP. It is known that equine PRP produced with the E-PET TM system contains leucocytes, therefore the product is also called L-PRP [25]. human subchondral mesenchymal progenitor cells [19] and human mesenchymal stem cells [20] as well as rabbit mesenchymal stem cells [21].…”

“…Interestingly, these cells remained intact after freezing at À20°C. It is known that equine PRP produced with the E-PET TM system contains leucocytes, therefore the product is also called L-PRP [25]. Eosinophils are especially enriched compared with the base line [14].…”

Platelet-rich plasma (PRP) therapy: An approach in reproductive medicine based on successful animal models

“…Examples include using PRP in horses for orthopedic therapy (musculoskeletal lesions) and tenodesmic lesions (Scala et al, 2014;Brossi et al, 2015), and using PRP in dogs for orthopedic therapy of ligament rupture by intra-articular injections (Silva et al, 2013;Vilar et al, 2013). Groups of veterinary researchers have studied the biology of PRP more extensively (mostly cellular and molecular studies) in horses (Rinnovati et al, 2016;Giraldo et al, 2015;Hessel et al, 2015;Hauschild et al, 2017) than in dogs (Silva et al, 2012;Carr et al, 2016;Frye et al, 2016). Scully et al (2018) reviewed the PRP applications in animal models for regenerative tissue, and they reported some important observations in rats (Hammond et al, 2009Martins et al, 2016;Li et al, 2016;Pinheiro et al, 2016;Borrione et al, 2017) using the application of PRP on a flexor sublimis lesion and tibialis anterior under muscle strain.…”