Short-term toxicity of bis(tri-n-butyltin)oxide in flounder (Platichthys flesus): Pathology and immune function

Grinwis, Guy C. M.; Boonstra, André; Brandhof, E.J. van den; Dormans, J. A. M. A.; Engelsma, Marc; Kuiper, Raoul V.; Loveren, Henk Van; Wester, Piet W.; Vaal, Manon; Vethaak, A.D.; Vos, J.G.

doi:10.1016/s0166-445x(97)00100-8

Cited by 56 publications

(19 citation statements)

References 39 publications

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“…These findings have been discussed in the context of immunomodulative effects of environmental chemicals, especially in coastal and estuarine areas (Sindermann 1996;Grinwis et al 1998). Thus, there is a demand for reliable tests which are able to reflect the status of immunocompetence in fish.…”

Section: Introductionmentioning

confidence: 99%

Acid phosphatase activity in liver macrophage aggregates as a marker for pollution-induced immunomodulation of the non-specific immune response in fish

Broeg

2003

Helgoland Marine Research

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The activity of acid phosphatase in liver macrophage aggregates (MA-AP) of different fish species was used as a marker for a pollution-induced modulation of the digestive capacity of phagocytes, since functions of the non-specific immune response play a central role in the maintenance of animals' health. Based upon the investigation of more than 900 individual flounders (Platichthys flesus) and mullets (Liza aurata), natural variations, gender-specific differences and pollution-induced alterations in AP activity are demonstrated in this study. MA-AP activity was dependent on temperature and season but, nevertheless, distinctions between differently polluted areas were visible in all sampling campaigns with lowest MA-AP activity in fish from the polluted areas of the German Bight and the Israeli coast of the Mediterranean Sea. For organochlorine contaminants, as well as for mercury and copper, a significant correlation could be observed between residue concentrations in fish tissues and MA-AP activity. In all cases, except mercury which showed a positive correlation, AP activity was suppressed in animals with a high contaminant burden. MA-AP activity turned out to give reliable and consistent results for a quantification of immunomodulation in both fish species.

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Section: Introductionmentioning

confidence: 99%

Acid phosphatase activity in liver macrophage aggregates as a marker for pollution-induced immunomodulation of the non-specific immune response in fish

Broeg

2003

Helgoland Marine Research

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“…These contaminants cause deleterious changes in cellular structures, including the epithelium and pillar cells (Laurent and Perry 1991;Randall et al 1996;Van Veld et al 1997). For example: bis(tri-n-butyltin)oxide produces gill lesions in flounder (Platichthys flesus) (Grinwis et al 1998) and N-methyl-N´-nitro-N-nitrosoguanidine induces branchial neoplasms in medaka (Oryzias latipes) (Brittelli et al 1985). Additional changes in the gill have been attributed to aromatic hydrocarbons (AHs) (Gagnon and Holdway 1999) and various other contaminants (Abel 1974;Teh et al 1997).…”

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confidence: 99%

Structural changes in gill DNA reveal the effects of contaminants on Puget Sound fish.

Malins

Stegeman

Anderson

et al. 2004

Environ Health Perspect

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Structural differences were identified in gill DNA from two groups of English sole collected from Puget Sound, Washington, in October 2000. One group was from the industrialized Duwamish River (DR) in Seattle and the other from relatively clean Quartermaster Harbor (QMH). Chemical markers of sediment contamination [e.g., polynuclear aromatic hydrocarbons (PAHs) and polychlorinated biphenyls (PCBs)] established that the DR was substantially more contaminated than QMH. The levels of these chemicals in the sediments of both sites were consistent with levels of cytochrome P450 1A (CYP1A) expression in the gills of English sole from the same sites. Structural differences in gill DNA between the groups were evinced via statistical models of Fourier transform-infrared (FT-IR) spectra. Marked structural damage was found in the gill DNA of the DR fish as reflected in differences in base functional groups (e.g., C-O and NH 2 ) and conformational properties (e.g., arising from perturbations in vertical base stacking interactions). These DNA differences were used to discriminate between the two fish groups through principal components analysis of mean FT-IR spectra. In addition, logistic regression analysis allowed for the development of a "DNA damage index" to assess the effects of contaminants on the gill. The evidence implies that environmental chemicals contribute to the DNA changes in the gill. The damaged DNA is a promising marker for identifying, through gill biopsies, contaminant effects on fish.

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“…widely distributed, lives in close contact with the sediment and is tolerant of changes in salinity which enables the fish to invade estuaries as well. Studies on flounder focus on chemical (Grinwis et al 2000), pathological and parasitological (Broeg et al 1999) analysis but also comprise measurements of innate immune parameters (Pulsford et al 1995;Boonstra et al 1996;Grinwis et al 1998). Among these, serum lysozyme activity and responses of phagocytic cells were most promising candidates for biomarkers.…”

Section: Discussionmentioning

confidence: 99%

Measuring some flounder ( Platichthys flesus L.) innate immune responses to be incorporated in effect biomonitoring concepts

Skouras

Steinhagen

2003

Helgoland Marine Research

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For an implementation of innate immune responses of flounder (Platichthys flesus) in an integrated biological effect monitoring concept, leucocytes were isolated from peripheral blood, head kidney and spleen, and analysed for their capacity to mount a respiratory burst response upon phorbol ester stimulation. Responding cells were identified by reduced nitro-blue-tetrazolium salt deposits and by dihydro-rhodamine fluorescence in light microscope and flow cytometric analysis. Responding cells were found in head kidney derived cell suspensions rather than in peripheral blood or spleen. Parallel cytometric and microscopic analysis indicated that responding cells had a granulocyte or monocyte morphology, were alpha-naphtyl-esterase or myeloperoxidase positive and in flow cytometry exhibited a characteristic forward and side scatter (FSC/SSC) pattern. These cells represented 30-40% of head kidney derived cell suspensions and only 4-5 % of peripheral blood and spleen. In order to reduce sampling effort in field studies, leucocyte cell suspensions derived from flounder head kidney could be used in respiratory burst assays without further enrichment protocols. This paper combines, for the first time, conventional and cytometric analysis of phagocytes derived from flounder peripheral blood and head kidney.

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Short-term toxicity of bis(tri-n-butyltin)oxide in flounder (Platichthys flesus): Pathology and immune function

Cited by 56 publications

References 39 publications

Acid phosphatase activity in liver macrophage aggregates as a marker for pollution-induced immunomodulation of the non-specific immune response in fish

Acid phosphatase activity in liver macrophage aggregates as a marker for pollution-induced immunomodulation of the non-specific immune response in fish

Structural changes in gill DNA reveal the effects of contaminants on Puget Sound fish.

Measuring some flounder ( Platichthys flesus L.) innate immune responses to be incorporated in effect biomonitoring concepts

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