Short wavelength fundus autofluorescence versus near-infrared fundus autofluorescence, with microperimetric correspondence, in patients with geographic atrophy due to age-related macular degeneration

Pilotto, Elisabetta; Vujosevic, Stela; Melis, R.; Convento, Enrica; Sportiello, Patrik; Alemany-Rubio, Ernesto; Segalina, Sara; Midena, Edoardo

doi:10.1136/bjo.2010.187344

Cited by 48 publications

(56 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, in highly elevated tumors, artifacts related to the technique may limit the analysis [ 15 ]. It has been suggested that longer and deeper wavelengths, such as NIR, could provide more information about the pigment composition of choroidal melanocytic lesions [ 3 ]. With NIR-AF, the melanin distribution can be explored, as oxidized melanin or compounds closely associated with melanin.…”

Section: Choroidal Melanomamentioning

confidence: 99%

“…Above the age of 70, as much as 20 % of the free cytoplasmic space of the RPE cells is occupied by LF granules but also by melanolipofuscin granules (MLF), a compound product of LF and melanin [ 2 ]. Also MLF acts as a fl uorophore, needing specifi c wavelengths of excitation and cutoff fi lters for emitting light (different from LF) to obtain the MLFrelated emission peak [ 3 ]. MLF is present in RPE in physiologic conditions, but increases in different pathologic conditions as well as LF.…”

Section: The Origin Of Autofl Uorescencementioning

confidence: 99%

“…MLF is present in RPE in physiologic conditions, but increases in different pathologic conditions as well as LF. Roughly all disorders causing RPE changes by direct or indirect involvement, as well as any disease affecting RPE metabolism, may cause a change in physiologic fundus AF by changing the amount of RPE-related fl uorophores [ 3 ].…”

Section: The Origin Of Autofl Uorescencementioning

confidence: 99%

“…Physiologic NIR-AF is characterized by the dark appearance of optic disc and retinal vessels [ 3 ]. The NIR-AF signal is maximal at the posterior pole, with a peak at the fovea and a marked decline toward the peripheral regions [ 1 ].…”

Section: Near-infrared Afmentioning

confidence: 99%

See 3 more Smart Citations

Diagnostic Techniques: Autofluorescence

Midena

Pilotto

Parrozzani

2013

Clinical Ophthalmic Oncology

View full text Add to dashboard Cite

Section: Choroidal Melanomamentioning

confidence: 99%

Section: The Origin Of Autofl Uorescencementioning

confidence: 99%

Section: The Origin Of Autofl Uorescencementioning

confidence: 99%

Section: Near-infrared Afmentioning

confidence: 99%

See 2 more Smart Citations

Diagnostic Techniques: Autofluorescence

Midena

Pilotto

Parrozzani

2013

Clinical Ophthalmic Oncology

View full text Add to dashboard Cite

“…For accurate calculation of juxtafoveal geographic atrophy, NI-AF should be performed in conjunction with BL-AF imaging to avoid artefactual overestimation of the area of RPE cell loss when BL-AF is used alone. 14 The Heidelberg retina angiography (HRA) and HRA2 (Heidelberg Engineering, Heidelberg, Germany) were the first commercial devices used for the acquisition of BL-AF and NI-AF. 12Y14 However, these devices are now superseded by Spectralis HRA + OCT (Heidelberg Engineering) in most clinics.…”

mentioning

confidence: 99%

Near-Infrared Autofluorescence Imaging in Geographic Atrophy Using Spectralis Single and Combined Wavelength Modes

Chen¹,

Khoo²,

Tang³

2015

Asia-Pacific Journal of Ophthalmology

View full text Add to dashboard Cite

show abstract

Microperimetry Features of Geographic Atrophy Identified With En Face Optical Coherence Tomography

et al. 2016

View full text Add to dashboard Cite

IMPORTANCE Progressive geographic atrophy (GA) of the retinal pigment epithelium leads to loss of central vision. To identify GA in age-related macular degeneration and assess treatment, correlation of function observed on microperimetry with structure observed on optical coherence tomographic (OCT) images may be of value.OBJECTIVE To characterize the microperimetric function of GA as identified from en face OCT imaging. DESIGN, SETTING, AND PARTICIPANTSIn a case-series study, 20 patients (22 eyes) entered the study at the University of Padova according to preplanned conditions. From March 1 to July 30, 2014, en face OCT images were obtained at the outer retinal layer and choroidal layer levels. The microperimetry sensitivity map was superimposed on the en face OCT images, which had been used to measure GA areas. Relative and dense scotoma rates were calculated in the GA areas. After data collection, the study eyes were divided into 3 groups according to the macular residual mean sensitivity. MAIN OUTCOMES AND MEASURESRetinal sensitivity measured by microperimetry within areas of GA identified by en face OCT images. RESULTS Twenty patients (5 men and 15 women) were included in the study, with a mean (SD) age of 79.5 (7.0) years (range, 69-98 years). Macular residual mean retinal sensitivity was less than 5 dB in 7 eyes (group 1), 5 to 10 dB in 9 eyes (group 2), and greater than 10 dB in 6 eyes (group 3). Mean (SD) GA area differed among the groups at the outer retinal (13.13 [5.03] mm 2 [range, 5.75-21.04 mm 2 ] in group 1; 7.80 [3.25] mm 2 [range, 3.31-13.52 mm 2 ] in group 2; and 3.94 [2.35] mm 2 [range, 1.46-7.90 mm 2 ] in group 3; P = .001) and choroidal (11.83 [5.55] mm 2 [range, 4.55-22.14 mm 2 ] in group 1; 7.00 [4.29] mm 2 [range, 0.90-13.83 mm 2 ] in group 2; and 3.27 [2.29] mm 2 [range, 0.91-7.23 mm 2 ] in group 3; P = .007) layer levels. Mean (SD) GA area imaged at the outer retinal layer level was significantly larger than that imaged at the choroidal level in group 3 (difference, 0.67 mm 2 ; 95% CI, 0.31-1.03 mm 2 ; P = .005), but not in groups 1 or 2. Mean (SD) rate of relative scotoma was significantly higher in the GA area imaged at the outer retinal layer level than at the choroidal level in group 3 (47.70% [31.30%] [range, 13.60%-100%] vs 34.00% [37.30%] [range, 0%-100%]; difference, 13.74%; 95% CI, 3.84%-23.63%; P = .02), but not in groups 1 or 2. CONCLUSIONS AND RELEVANCEIn the early stage of GA, when retinal sensitivity is relatively good, these data suggest that the GA area imaged on en face OCT at the outer retinal level correctly detects the wide functional degenerative involvement of the photoreceptors. These findings provide novel data that correlate function and structure, which may be of value when assessing treatments that might prevent or reduce the rate of growth of GA.

show abstract

Short wavelength fundus autofluorescence versus near-infrared fundus autofluorescence, with microperimetric correspondence, in patients with geographic atrophy due to age-related macular degeneration

Cited by 48 publications

References 20 publications

Diagnostic Techniques: Autofluorescence

Diagnostic Techniques: Autofluorescence

Near-Infrared Autofluorescence Imaging in Geographic Atrophy Using Spectralis Single and Combined Wavelength Modes

Microperimetry Features of Geographic Atrophy Identified With En Face Optical Coherence Tomography

Contact Info

Product

Resources

About