Shot hole disease on Prunus laurocerasus caused by Neofusicoccum parvum in Serbia

Abstract

Between 2010 and 2014, symptoms of a shot hole disease were observed on cherry laurel (Prunus laurocerasus L.) trees and shrubs in parks and other public plantings in Belgrade, Serbia. Ten symptomatic leaves were collected from each of the diseased plants and the associated fungus isolated and identified using multigene phylogenetic analyses and asexual morphological characters. The pathogen was identified as Neofusicoccum parvum. The same symptoms were produced when the pathogen was inoculated on test plants. To the best of our knowledge, this is the first report of N. parvum causing shot hole disease on P. laurocerasus.

Host specificity of co‐infecting Botryosphaeriaceae on ornamental and forest trees in the Western Balkans

“…Diversity indexes were calculated in R (R Core Team, ), using statistical package Vegan v. 2.2.1 (Oksanen et al., ). Botryosphaeriaceae species diversity in the Western Balkans was assessed using the whole collection of Botryosphaeriaceae isolates from the Western Balkans region, including isolates identified in this and in previous studies (Figure S1; Zlatković et al., ,b, ).…”

“…In contrast to a large number of studies investigating the Botryosphaeriaceae diversity and impact in the Mediterranean region (MR) and in the tropics (e.g., Alves, Linaldeddu, Deidda, Scanu, & Phillips, ; Burgess, Barber, & Hardy, ; Jami et al., ; Rodas, Slippers, Gryzenhout, & Wingfield, ), less attention has been given to the Botryosphaeriaceae occurring in the Continental climate‐type region (CR). Apart from some recent studies from the Balkans (e.g., Piškur et al., ; Zlatković, Keča, Wingfield, Jami, & Slippers, ; Zlatković et al., , ), most research on the Botryosphaeriaceae conducted in the CR has been focused on the pine pathogen, D. sapinea (e.g., Jankovský & Palovćiková, ; Karadžić & Milijašević, ; Fabre et al., ; Adamson et al., ).…”

“…In the Western Balkans, N. parvum , Botryosphaeria dothidea and D. seriata have been found to cause disease on V. vinifera , Malus domestica and Olea europaea (Kaliterna, Milicevic, Bencic, & Duralija, ; Kaliterna, Milicevic, Ivic, Bencic, & Mesic, ; Latinović, Mazzaglia, Latinović, Ivanović, & Gleason, ; Vasić, Duduk, Vico, & Ivanović, ). Furthermore, a large number of Botryosphaeriaceae species have recently been found on various diseased trees in this region, including B. dothidea , N. parvum , D. sapinea , Diplodia mutila , D. seriata , Dothiorella sarmentorum , Dothiorella sp., Dothiorella omnivora , P. cupressi and S. visci (Zlatković et al., ,b, ). These species have been found associated with both angiosperms and gymnosperms belonging to various families, that is Rosaceae, Fagaceae, Cupressaceae, Pinaceae, Oleaceae, Sapindaceae, Santalaceae, Pittosporaceae and Myrtaceae.…”

“…A selection of these isolates and isolates of the Botryosphaeriaceae identified in the previous study (Zlatković et al., ) was then used to conduct reciprocal inoculations on the hosts from which the Botryosphaeriaceae had been isolated, as well as on C. lawsoniana , Cedrus deodara, Picea omorika , E. grandis and Pinus patula from which some or all of the Botryosphaeriaceae species were not isolated. The overlapping patterns of all Botryosphaeriaceae species identified in the Western Balkans (Zlatković et al., ,b, ) were assessed at an individual plant, host species and geographic level.…”

Occurrence and pathogenicity of Phytophthora × cambivora on Prunus laurocerasus in Serbia

“…Due to its high ornamental value and non‐problematic growth, P. laurocerasus is also worldwide used as an ornamental plant. Despite being generally relatively resistant to pathogens, cherry laurel plants can suffer from diseases caused by several pathogenic organisms, in particular, species within the Botryosphaeriaceae family (Zlatković, Keča, Wingfield, Jami, & Slippers, ). In Italy, Phytophthora cryptogea was found causing root rot and dieback of cherry laurel plants (Vettraino et al., ).…”