2014
DOI: 10.3892/or.2014.3267
|View full text |Cite
|
Sign up to set email alerts
|

shRNA targeting Bmi-1 sensitizes CD44+ nasopharyngeal cancer stem-like cells to radiotherapy

Abstract: Accumulating evidence indicates that cancer stem cells (CSCs) are involved in resistance to radiation therapy (RT). Bmi-1, a member of the Polycomb family of transcriptional repressors, is essential for maintaining the self-renewal abilities of stem cells and overexpression of Bmi-1 correlates with cancer therapy failure. Our previous study identified that the CD44+ nasopharyngeal cancer (NPC) cells may be assumed as one of markers of nasopharyngeal carcinoma cancer stem cell-like cells (CSC-LCs) and Bmi-1 is … Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
2
1

Citation Types

3
12
0

Year Published

2015
2015
2024
2024

Publication Types

Select...
7

Relationship

1
6

Authors

Journals

citations
Cited by 12 publications
(15 citation statements)
references
References 32 publications
3
12
0
Order By: Relevance
“…Although we previously confirmed that silencing of Bmi-1 resulted in CD44 + NPC CSC-LC sensitivity to radiotherapy (23), the role of Bmi-1 in NPC development has not been fully elucidated, and whether Bmi-1 can serve as a therapeutic target for NPC remains to be assessed. In the present study, scratch wound healing assay, together with Transwell migration and invasion assays were used to observe invasion and migration capacity; flow cytometry was used to analyze the cell apoptosis status; tumorigenesis in nude mice was used to assess tumorigenicity; and immunohistochemical techniques were used to detect the expression of CD44 in tumor tissues.…”
Section: Downregulation Of Bmi-1 Is Associated With Suppressed Tumorimentioning
confidence: 84%
See 2 more Smart Citations
“…Although we previously confirmed that silencing of Bmi-1 resulted in CD44 + NPC CSC-LC sensitivity to radiotherapy (23), the role of Bmi-1 in NPC development has not been fully elucidated, and whether Bmi-1 can serve as a therapeutic target for NPC remains to be assessed. In the present study, scratch wound healing assay, together with Transwell migration and invasion assays were used to observe invasion and migration capacity; flow cytometry was used to analyze the cell apoptosis status; tumorigenesis in nude mice was used to assess tumorigenicity; and immunohistochemical techniques were used to detect the expression of CD44 in tumor tissues.…”
Section: Downregulation Of Bmi-1 Is Associated With Suppressed Tumorimentioning
confidence: 84%
“…Cell culture. A stable Bmi-1-knockdown (KD) cell line was obtained by transfecting CD44 + cells with retroviral vector Bmi-1 short hairpin RNA (shRNA) (23). Negative control cells (NC) were transfected with an empty retroviral vector and the cells without transfection were used as the blank control (CoN).…”
Section: Methodsmentioning
confidence: 99%
See 1 more Smart Citation
“…In normal human keratinocytes, BMI1 elicits radioprotective effects by mitigating the genotoxic effects of ionizing radiation (IR) (15). In nasopharyngeal carcinoma cells, targeting BMI1 expression increases their susceptibility to radiation through the induction of oxidative stress and apoptosis (13). Elevated expression of BMI1 has been shown to radioprotect CD133-positive cancer-initiating neural stem cells through recruitment of DNA damage response (DDR) machinery to DSBs after exposure to radiation (16).…”
Section: Introductionmentioning
confidence: 99%
“…Intensity‐modulated radiation therapy and active anticancer agents are standard treatment options for NPC . In recent years, cancer stem cells and gene therapy are new concepts and promising strategies for NPCs, but these new technologies have yet to be applied in the clinic . Similar to other types of malignant tumour, TNM stages of NPC are significantly correlated with the treatment efficacy and the prognosis of the disease.…”
Section: Introductionmentioning
confidence: 99%