sHsp22.6, an intronless small heat shock protein gene, is involved in stress defence and development in Apis cerana cerana

Zhang, Yuanying; Liu, Yaling; Guo, Xing; Li, Yalu; Gao, Hongjiang; Guo, Xingqi; Xu, Baohua

doi:10.1016/j.ibmb.2014.06.007

Cited by 61 publications

(51 citation statements)

References 63 publications

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“…Septic injury caused by the E. coli infection induced a strong expression of Tchsp68 and Tchsp18.3 , which had been reported in T. castaneum (Altincicek, Knorr, & Vilcinskas, ). In Apis cerana cerana , Acchsp22.6 was significantly upregulated by Ascosphaera apis treatment for 24–72 hr (Zhang, Liu et al., ). However, few studies have analysed the orthologous shsps expression in relation to pathogen stresses in insects.…”

Section: Discussionmentioning

confidence: 99%

Characterization and functional analysis of hsp21.8b: An orthologous small heat shock protein gene in Tribolium castaneum

Xie

Xiong

et al. 2018

J Applied Entomology

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Small heat shock proteins (sHSPs) act as molecular chaperones and are widely distributed in all kinds of organisms. Comparative analysis revealed that an orthologous shsp was present during insect evolution. Here, hsp21.8b, one insect orthologous shsp, had been identified in Tribolium castaneum. Quantitative real‐time PCR illustrated that Tchsp21.8b was expressed in all developmental stages, along with the lowest expression at early embryonic stage and relative high expression at other stages especially in late eggs and late pupae. In the adult period, Tchsp21.8b exhibited the highest expression level in central nervous system and followed in elytron, epidermis, ovary and fat body. Moreover, it was upregulated 3.39‐fold in response to enhanced heat stress (45°C) for 4 hr but not to cold stress (4°C) and was upregulated by 1.73‐ to 1.94‐fold under ultraviolet (UV) exposure during 4–6 hr. It was also downregulated by 20.8%–41.8% under starvation in 3 days and had a “down‐up‐down” trend under the pathogen stresses. Larval RNA interference of Tchsp21.8b caused 40.6% insects mortality and reduced the oviposition amount by 66.0% and only 21.0% of the ds‐Tchsp21.8b eggs could hatch into larvae. These results suggested that as an orthologous shsp, Tchsp21.8b not only plays important roles in the growth, development and fecundity of T. castaneum but with the competence to resist the environment stresses, although the response is relatively weak compared to other hsps. Results from this study also uncovered the functions of the orthologous shsp in the development and anti‐stresses ability of T. castanuem. It provided more scientific evidence for revealing the physiological mechanisms of shsps of the insects and enhanced the capabilities to control different pests.

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Section: Discussionmentioning

confidence: 99%

Characterization and functional analysis of hsp21.8b: An orthologous small heat shock protein gene in Tribolium castaneum

Xie

Xiong

et al. 2018

J Applied Entomology

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“…Recent studies showed that the sHSPs in insects also play various roles. For instance, in Apis cerana cerana , hsp22.6 was significantly up‐regulated by abiotic stresses, such as temperature (4°C, 16°C, 42°C), pesticides (cyhalothrin, pyridaben), oxidative stress (UV, H 2 O 2 ) and heavy metals (CdCl 2 ), as well as biotic stress, such as 20‐hydroxyecdysone (20E), Ascosphaera apis treatment (Zhang et al ., ). In D. melanogaster , over‐expressing the mitochondrial hsp22 increased the resistance to oxidative stress and extended lifespan by 32%.…”

Section: Introductionmentioning

confidence: 97%

Characterization and functional analysis of hsp18.3 gene in the red flour beetle, Tribolium castaneum

Xie

Zhai

et al. 2017

Insect Science

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Small heat shock proteins (sHSPs) are diverse and mainly function as molecular chaperones to protect organisms and cells from various stresses. In this study, hsp18.3, one Tribolium castaneum species-specific shsp, has been identified. Quantitative real-time polymerase chain reaction illustrated that Tchsp18.3 is expressed in all developmental stages, and is highly expressed at early pupal and late adult stages, while it is highly expressed in ovary and fat body at the adult period. Moreover, it was up-regulated 4532 ± 396-fold in response to enhanced heat stress but not to cold stress; meanwhile the lifespan of adults in ds-Tchsp18.3 group reduced by 15.8% from control group under starvation. Laval RNA interference (RNAi) of Tchsp18.3 caused 86.1% ± 4.5% arrested pupal eclosion and revealed that Tchsp18.3 played an important role in insect development. In addition, parental RNAi of Tchsp18.3 reduced the oviposition amount by 94.7%. These results suggest that Tchsp18.3 is not only essential for the resistance to heat and starvation stress, but also is critical for normal development and reproduction in T. castaneum.

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“…Several sHSPs are known in insects, with a variable number of members appearing in different species. Drosophila melanogaster was the first insect in which they were described (Southgate et al, 1983), though the sHSPs have also been identified and described in lepidopterans such as Bombyx mori (Sakano et al, 2006;Li et al, 2009;Wang et al, 2014), and hymenopterans such Apis cerana cerana (Liu et al, 2012Zhang et al, 2014). However, in most insect species exists only scattered information and a few members identified.…”

Section: Introductionmentioning

confidence: 99%

Characterization of six small HSP genes from Chironomus riparius (Diptera, Chironomidae): Differential expression under conditions of normal growth and heat-induced stress

Martín-Folgar

Fuente

Morcillo

et al. 2015

Comparative Biochemistry and Physiology Part A: Molecular &

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sHsp22.6, an intronless small heat shock protein gene, is involved in stress defence and development in Apis cerana cerana

Cited by 61 publications

References 63 publications

Characterization and functional analysis of hsp21.8b: An orthologous small heat shock protein gene in Tribolium castaneum

Characterization and functional analysis of hsp21.8b: An orthologous small heat shock protein gene in Tribolium castaneum

Characterization and functional analysis of hsp18.3 gene in the red flour beetle, Tribolium castaneum

Characterization of six small HSP genes from Chironomus riparius (Diptera, Chironomidae): Differential expression under conditions of normal growth and heat-induced stress

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