Sialic acids acquired by Pseudomonas aeruginosa are involved in reduced complement deposition and siglec mediated host‐cell recognition

Abstract

The opportunism of Pseudomonas aeruginosa (PA) in immunocompromised hosts prompted us to explore the potential role of sialic acids (Sia) in this phenomenon. Culture of PA in the presence of exogenous Sia resulted in linkage-specific incorporation of Sia which was associated with decreased complement deposition on the bacteria. Sia acquired by PA mediated enhanced binding of bacteria to recombinant-CHO cells expressing human siglec-7 or siglec-9, as well as to human NK-cells and monocytes naturally expressing these siglecs. Therefore, Sia may be acquired by PA in the host and contribute to bacterial pathogenicity and host-cell interactions via reduction of complement deposition and siglec-dependent recognition.

Retracted Science and the Retraction Index

“…In addition, the passage of time may have reduced the significance of the reported findings such that the article is no longer assigned high priority by the journal. Nevertheless, there are instances in which a retracted article has been corrected and republished by the same or another journal (17,30,43,44,46,47). Scientists, it would seem, also believe in redemption.…”

Sialylation of Outer Membrane Porin Protein D: A Mechanistic Basis of Antibiotic Uptake in Pseudomonas aeruginosa

“…ϪSias were derivatized with 1,2-diamino-4,5-methylenedioxybenzene (DMB) (11,19,45). DMB-Sias were analyzed by fluorimetric reverse-phase HPLC analysis on a RP-18 column (LichroCART, Merck, Germany) using isocratic elution (19,45) and compared with Sias purified from BSM.…”

“…DMB-Sias were analyzed by fluorimetric reverse-phase HPLC analysis on a RP-18 column (LichroCART, Merck, Germany) using isocratic elution (19,45) and compared with Sias purified from BSM. Fluorescence was detected using an excitation and emission wavelength of 373 nm and 448 nm respectively.…”

“…We have reported earlier the presence of linkage-specific Sias on PA. Normal human serum (NHS) is possibly one of the sources of these Sias (19). PA utilizes these Sias to interact through siglecs present on the surface of different immune cells.…”

Genomic and Metabolic Profiling of Nonulosonic Acids in Vibrionaceae Reveal Biochemical Phenotypes of Allelic Divergence in Vibrio vulnificus

“…In their various locations on microbial surfaces, different NulO structures have been implicated in a variety of host-microbe interactions. NulOs of the sialic, legionaminic, and pseudaminic acid types are involved in bacterial behaviors like biofilm formation, autoagglutination, and motility (1,15,18,45,52), as well as direct protein-carbohydrate interactions between hosts and pathogens (8,9,26,30,52). In particular, sialic acid-containing bacterial glycans participate in strategies of immune suppression and subversion, likely contributing to clinical conditions ranging from urogenital, airway, and ear infections to systemic bacteremia, meningitis, and the induction of autoimmunity (1,24,32,56,57,63,64).…”