Sib‐parentage testing using molecular markers when parents are unknown

García, Dolores Brandis; Carleos, Carlos; Parra, Denis; Cañón, Javier

doi:10.1046/j.1365-2052.2002.00882.x

Cited by 10 publications

(12 citation statements)

References 8 publications

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“…Memory‐based kinship assignments assessment was made at two levels. Sire–offspring and dam–offspring dyads were examined using the likelihood approach (Garcia et al. 2002).…”

Section: Methodsmentioning

confidence: 99%

“…2007) (http://www.fieldgenetics.com/). Here, the power of a set of genetic markers to exclude candidate parents was systematically computed into an EP (Garcia et al. 2002).…”

Section: Methodsmentioning

confidence: 99%

“…Memory-based kinship assignments assessment was made at two levels. Sire-offspring and damoffspring dyads were examined using the likelihood approach (Garcia et al 2002). The average EP for non-exclusion of candidate parents was calculated using the program cervus version 3.0 (Kalinowski et al 2007) (http://www.fieldgenetics.com/).…”

Section: Statistical Analyses and Pedigree Reconstructionmentioning

confidence: 99%

“…The average EP for non-exclusion of candidate parents was calculated using the program cervus version 3.0 (Kalinowski et al 2007) (http://www.fieldgenetics.com/). Here, the power of a set of genetic markers to exclude candidate parents was systematically computed into an EP (Garcia et al 2002). This exclusion technique is based on Mendelian rules of inheritance and uses incompatibilities between parents and offspring to reject particular parent-offspring hypotheses (Jones & Arden 2003).…”

Section: Statistical Analyses and Pedigree Reconstructionmentioning

confidence: 99%

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Accuracy of pastoralists’ memory‐based kinship assignment of Ankole cattle: a microsatellite DNA analysis

Kugonza

Kiwuwa

Mpairwe

et al. 2011

J Animal Breeding Genetics

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This study aimed to estimate the level of relatedness within Ankole cattle herds using autosomal microsatellite markers and to assess the accuracy of relationship assignment based on farmers' memory. Eight cattle populations (four from each of two counties in Mbarara district in Uganda) were studied. Cattle in each population shared varying degrees of relatedness (first-, second- and third-degree relatives and unrelated individuals). Only memory-based kinship assignments which farmers knew with some confidence were tested in this experiment. DNA isolated from the blood of a subsample of 304 animals was analysed using 19 microsatellite markers. Average within population relatedness coefficients ranged from 0.010 ± 0.005 (Nshaara) to 0.067 ± 0.004 (Tayebwa). An exclusion probability of 99.9% was observed for both sire-offspring and dam-offspring relationships using the entire panel of 19 markers. Confidence from likelihood tests performed on 292 dyads showed that first-degree relatives were more easily correctly assigned by farmers than second-degree ones (p < 0.01), which were also easier to assign than third-degree relatives (p < 0.01). Accuracy of kinship assignment by the farmers was 91.9% ± 5.0 for dam-offspring dyads, 85.5% ± 3.4 for sire-offspring dyads, 75.6% ± 12.3 for half-sib and 60.0% ± 5.0 for grand dam-grand offspring dyads. Herd size, number of dyads assigned and length of time spent by the herder with their cattle population did not correlate with error in memorizing relationships. However, herd size strongly correlated with number of dyads assigned by the herder (r = 0.967, p < 0.001). Overall, we conclude that memorized records of pastoralists can be used to trace relationships and for pedigree reconstruction within Ankole cattle populations, but with the awareness that herd size constrains the number of kinship assignments remembered by the farmer.

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“…Memory‐based kinship assignments assessment was made at two levels. Sire–offspring and dam–offspring dyads were examined using the likelihood approach (Garcia et al. 2002).…”

Section: Methodsmentioning

confidence: 99%

“…2007) (http://www.fieldgenetics.com/). Here, the power of a set of genetic markers to exclude candidate parents was systematically computed into an EP (Garcia et al. 2002).…”

Section: Methodsmentioning

confidence: 99%

Section: Statistical Analyses and Pedigree Reconstructionmentioning

confidence: 99%

Section: Statistical Analyses and Pedigree Reconstructionmentioning

confidence: 99%

See 2 more Smart Citations

Accuracy of pastoralists’ memory‐based kinship assignment of Ankole cattle: a microsatellite DNA analysis

Kugonza

Kiwuwa

Mpairwe

et al. 2011

J Animal Breeding Genetics

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“…Studies focused on the genetic characterization of species and breeds of domestic animals have a wide range of possible applications. These studies have been carried out to investigate the origin and course of domestication of different species and breeds (Braend and Roed, 1987;Tanabe, 1990;Troy, 2001;Savolainen et al, 2002), to characterize the breeds and lines (by estimation of genetic drift and distance) (Pihkanen et al, 1996;Tsuda et al, 1997), to design adequate breeding programs based upon the identification of genes which control economically relevant traits (Christensen et al, 1985;Andersson et al, 1987), for parentage control (Juneja et al,1987;Dostal and Stratil, 1994;Garcia et al, 2002), etc. Genetic characterization of autochthonous breeds in different geographical localities is of particular importance.…”

Section: Introductionmentioning

confidence: 99%

Genetic polymorphism of blood proteins in Yugoslav shepherd dog

Vladimir¹,

Slobodan²,

Mila³

et al. 2005

Acta vet. (Beogr.)

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The present study is aimed to provide the first insight into the genetic constitution of Yugoslav shepherd dog based upon analysis of blood proteins and enzyme polymorphism. A total of 60 Yugoslav shepherd dogs were included in the study and polymorphism of hemoglobin (Hb), acid phosphatase (Acp) superoxide dismutase (Sod), albumin (Al), and transferrine (If) was electrophoretically analysed. Hb system did not show polymorphism. FS and SS phenotypes of Acp were established with Acps allelic gene displaying significantly higher frequency (0.82) than AcpF (0.18). The analysis of Sod polymorphism showed SodAA and SodAB phenotypes controlled by SodA (0.74) and SodB (0.26) with clear predominance of SodA allele. Both Al allelic genes At (0.30) and Als(0.70), and all three possible phenotypes (F, FS, and S) were detected. Seven Tf phenotypes controlled by five allelic genes 77* Tf8, Tf, 77°, and Tf-were revealed which makes the Yugoslav shepherd dog the only dog breed displaying all five Tf genes. The associated gene frequencies were (0.13, 0.35, 0.06, 0.41 i 0.05), respectively. According to the calculated values of homogeneity index, Al and Tf were identified as highly polymorphic protein markers which is a result of importance for future genetic characterization of the Yugoslav shepherd dog. Significant deviations from Hardy-Weinberg equilibrium were detected at Sod and Tf loci these being primarily attributed to genetic drift, although crossbreeding should not be disregarded as a factor of possible importance as well

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Waste not, want not: Microsatellites remain an economical and informative technology for conservation genetics

Hauser

Athrey

Leberg

2021

Ecology and Evolution

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Comparisons of microsatellites and single-nucleotide polymorphisms (SNPs) have found that SNPs outperform microsatellites in population genetic analyses, questioning the continued utility of microsatellites in population and landscape genetics.Yet, highly polymorphic markers may be of value in species that have reduced genetic variation. This study repeated previous analyses that used microsatellites with SNPs developed from ddRAD sequencing in the black-capped vireo source-sink system.SNPs provided greater resolution of genetic diversity, population differentiation, and migrant detection but could not reconstruct parentage relationships due to insufficient heterozygosities. The biological inferences made by both sets of markers were similar: asymmetrical gene flow from source sites to the remaining sink sites. With the landscape genetic analyses, we found different results between the two molecular markers, but associations of the top environmental features (riparian, open habitat, agriculture, and human development) with dispersal estimates were shared between marker types. Despite the higher precision of SNPs, we find that microsatellites effectively uncover population processes and patterns and are superior for parentage analyses in this species with reduced genetic diversity. This study illustrates the continued applicability and relevance of microsatellites in population genetic research.

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Sib‐parentage testing using molecular markers when parents are unknown

Cited by 10 publications

References 8 publications

Accuracy of pastoralists’ memory‐based kinship assignment of Ankole cattle: a microsatellite DNA analysis

Accuracy of pastoralists’ memory‐based kinship assignment of Ankole cattle: a microsatellite DNA analysis

Genetic polymorphism of blood proteins in Yugoslav shepherd dog

Waste not, want not: Microsatellites remain an economical and informative technology for conservation genetics

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