Siderophore production by Pseudomonas aeruginosa FP6, a biocontrol strain for Rhizoctonia solani and Colletotrichum gloeosporioides causing diseases in chilli

BAKTHAVATCHALU, SASIREKHA; Shivakumar, Srividya

doi:10.1016/j.anres.2016.02.003

Cited by 88 publications

(67 citation statements)

References 14 publications

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“…Many researchers have reported fluorescent pseudomonads to be prolific producers of siderophores (Pandey et al 2005 ; Subramanian and Satyan 2014 ; Pattan et al 2017 ; Kotasthane et al 2017 ). In fact, P. aeruginosa are amongst the most efficient producers of siderophores reported from rhizosphere or other habitats as shown by this work also (de Villegas et al 2002 ; Unni et al 2014 ; Sasirekha and Srividya 2016 ). Strains of P. aeruginosa (including TO3, RB1, and PF23) were found to be most efficient siderophore producers in comparison with others.…”

Section: Resultssupporting

confidence: 76%

Modified microplate method for rapid and efficient estimation of siderophore produced by bacteria

2017

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In this study, siderophore production by various bacteria amongst the plant-growth-promoting rhizobacteria was quantified by a rapid and efficient method. In total, 23 siderophore-producing bacterial isolates/strains were taken to estimate their siderophore-producing ability by the standard method (chrome azurol sulphonate assay) as well as 96 well microplate method. Production of siderophore was estimated in percent siderophore unit by both the methods. It was observed that data obtained by both methods correlated positively with each other proving the correctness of microplate method. By the modified microplate method, siderophore production by several bacterial strains can be estimated both qualitatively and quantitatively at one go, saving time, chemicals, making it very less tedious, and also being cheaper in comparison with the method currently in use. The modified microtiter plate method as proposed here makes it far easier to screen the plant-growth-promoting character of plant-associated bacteria.

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Section: Resultssupporting

confidence: 76%

Modified microplate method for rapid and efficient estimation of siderophore produced by bacteria

2017

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“…These results are in harmony with the results obtained by Pàez et al, 2005 who reported that P. aeruginosa was more efficient than P. fluorescens in inhibiting some plant pathogenic fungi [39]. Also, Sasirekha and Srividya (2016) reported that siderophores-producing P. aeruginosa FP6 had antagonistic activity against R. solani [40]. Also, Islam et al, 2018 showed that P. aeruginosa RKA5 inhibited mycelia growth of F. oxysporum f. sp.…”

Section: Discussionsupporting

confidence: 83%

Maximization of Siderophores Production from Biocontrol Agents, Pseudomonas aeruginosa F2 and Pseudomonas fluorescens JY3 Using Batch and Exponential Fed-Batch Fermentation

et al. 2020

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Twenty fluorescent Pseudomonas isolates were tested for their ability to produce siderophores on chrome azurol S (CAS) agar plates and their antagonistic activity against six plant pathogenic fungal isolates was assessed. Scaling-up production of siderophores from the promising isolates, P. aeruginosa F2 and P. fluorescens JY3 was performed using batch and exponential fed-batch fermentation. Finally, culture broth of the investigated bacterial isolates was used for the preparation of two economical bioformulations for controlling Fusarium oxysporum and Rhizoctonia solani. The results showed that both isolates yielded high siderophore production and they were more effective in inhibiting the mycelial growth of the tested fungi compared to the other bacterial isolates. Exponential fed-batch fermentation gave higher siderophore concentrations (estimated in 10 µL), which reached 67.05% at 46 h and 45.59% at 48 h for isolates F2 and JY3, respectively, than batch fermentation. Formulated P. aeruginosa F2 and P. fluorescens JY3 decreased the damping-off percentage caused by F. oxysporum with the same percentage (80%), while, the reduction in damping-off percentage caused by R. solani reached 87.49% and 62.5% for F2 and JY3, respectively. Furthermore, both formulations increased the fresh and dry weight of shoots and roots of wheat plants. In conclusion, bio-friendly formulations of siderophore-producing fluorescent Pseudomonas isolates can be used as biocontrol agents for controlling some plant fungal diseases.

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“…Based on our data, the three strains use different way to preclude the entry of excess zinc ions. P. aeruginosa PAO1 significantly decreased the secretion of siderophore, which was also found to complex with zinc, nickel, cobalt and magnesium as well as iron [36,37]. P. putida universally reduced the expression of membrane transporters.…”

Section: Discussionmentioning

confidence: 98%

Determining the different mechanisms used by Pseudomonas strains to cope with minimal inhibitory concentrations of zinc via comparative transcriptomic and metabolic analyses

P¹,

Chen²,

Liao³

et al. 2020

Preprint

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Background Pseudomonas is one of the most diverse bacterial genera identified in soil, water, plants and clinical samples. Genome sequence analysis has indicated that this genus can be clustered into three lineages and ten groups. Each group can adopt different mechanisms to thrive under zinc-depleted or high-zinc conditions, two environments that are frequently encountered during their environmental propagation. Results The response mechanisms of three prominent Pseudomonas strains (Pseudomonas aeruginosa PAO1, Pseudomonas putida KT2440, and Pseudomonas fluorescence ATCC13525T) under high-zinc condition were compared using RNA-seq and ultra-performance liquid chromatography–tandem mass spectrometry analysis. Results demonstrated that the three strains shared only minimal similarity at the transcriptional level. Only four genes responsible for zinc efflux were commonly upregulated. P. aeruginosa PAO1 specifically downregulated the operons involved in siderophore synthesis and the genes that encode ribosomal protein, while upregulated the genes associated with antibiotic efflux and cell envelope biosynthesis. The membrane transporters in P. putida KT2440 were globally downregulated, indicating changes in cell permeability. Compared with P. aeruginosa PAO1 and P. putida KT2440, the most remarkable transcriptional variation in P. fluorescence ATCC13525 is the significant downregulation of the type VI secretion system. Metabolite quantitative analysis showed that low concentrations of the metabolites involved in central carbon metabolism and amino acid synthesis were detected in the three strains. Dipeptides containing branched-chain amino acids seems played an important role in alleviating stress caused by zinc ions. Conclusion The exclusion of cytoplasmic ions and reducing the intrusion of excess zinc are equally important for P. aeruginosa, P. putida, and P. fluorescence to withstand external zinc stress. Although similar metal efflux systems were commonly upregulated, these three strains apparently used different pathways to reduce zinc entry. In addition, zinc treatment can increase the difficulties of scavenging P. aeruginosa from its colonisation area, and reduce the effectiveness of P. fluorescence as a biocontrol agent.

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Siderophore production by Pseudomonas aeruginosa FP6, a biocontrol strain for Rhizoctonia solani and Colletotrichum gloeosporioides causing diseases in chilli

Cited by 88 publications

References 14 publications

Modified microplate method for rapid and efficient estimation of siderophore produced by bacteria

Modified microplate method for rapid and efficient estimation of siderophore produced by bacteria

Maximization of Siderophores Production from Biocontrol Agents, Pseudomonas aeruginosa F2 and Pseudomonas fluorescens JY3 Using Batch and Exponential Fed-Batch Fermentation

Determining the different mechanisms used by Pseudomonas strains to cope with minimal inhibitory concentrations of zinc via comparative transcriptomic and metabolic analyses

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