Sigma factor WhiG and its regulation constitute a target of a mutational phenomenon occurring during aerial mycelium growth in Streptomyces ambofaciens ATCC23877

Catakli, Sibel; Andrieux, Axelle; Decaris, Bernard; Dary, Annie

doi:10.1016/j.resmic.2004.12.001

Cited by 8 publications

(4 citation statements)

References 36 publications

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“…The finding that the dmdR1 gene overlaps with an antiparallel gene ( adm ) that shows 87% identity with a development‐regulated gene of S. aureofaciens [16] is really intriguing. The S. aureofaciens adm gene was found in a search for promoters that are recognized by the RpoZ sigma factor, a member of the WhiG family [17,18]. Nováková et al.…”

Section: Discussionmentioning

confidence: 99%

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Two overlapping antiparallel genes encoding the iron regulator DmdR1 and the Adm proteins control sidephore and antibiotic biosynthesis in Streptomyces coelicolor A3(2)

et al. 2009

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The dmdR1 gene of Streptomyces coelicolor encodes an important regulator of iron metabolism. An antiparallel gene (adm) homologous to a development‐regulated gene of Streptomyces aureofaciens has been found to overlap with dmdR1. Both proteins DmdR1 and Adm are formed in solid and liquid cultures of S. coelicolor A3(2). The purpose of this study was to assess possible interaction between the products of these two antiparallel genes. Two mutants with stop codons resulting in arrested translation of either DmdR1 or Adm were obtained by gene replacement and compared with a deletion mutant (ΔdmdR1/adm) that was defective in both genes. The deletion mutant was unable to form either protein, did not sporulate and lacked desferrioxamine, actinorhodin and undecylprodigiosin biosynthesis; biosynthesis of these compounds was recovered by complementation with dmdR1/adm genes. The mutant in which formation of Adm protein was arrested showed normal levels of DmdR1, lacked Adm and over‐produced the antibiotics undecylprodigiosin and actinorhodin (in MS medium), suggesting that Adm plays an important role in secondary metabolism. The mutant in which DmdR1 formation was arrested synthesized desferrioxamines in a constitutive (deregulated) manner, and produced relatively normal levels of antibiotics. In conclusion, our results suggest that there is a fine interplay of expression of these antiparallel genes, as observed for other genes that encode lethal proteins such as the toxin/antitoxin systems. The Adm protein seems to have a major effect on the control of secondary metabolism, and its formation is probably tightly controlled, as expected for a key regulator.

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Section: Discussionmentioning

confidence: 99%

“…1). This S. aureofaciens gene was found in a search for promoters that are recognized by the RpoZ sigma factor, a member of the WhiG family [17] of sigma factors [18,19]. Nováková et al.…”

Section: Introductionmentioning

confidence: 99%

Two overlapping antiparallel genes encoding the iron regulator DmdR1 and the Adm proteins control sidephore and antibiotic biosynthesis in Streptomyces coelicolor A3(2)

et al. 2009

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“…Although the S. coelicolor chromosome contains a prophage-like insertion upstream of whiG that is absent from S. avermitilis, the genes downstream are conserved and syntenous between the species. The whiG genes of several other streptomycetes have been shown to play a similar role in committing aerial hyphae to sporulation, and, where sequenced, all are highly conserved in their coding regions and in their promoters at least from À 1 to À 45, the region containing one of the two characterized BldD-binding sites in the whiG upstream region of S. coelicolor (Soliveri et al, 1993;Kormanec et al, 1994;Elliot et al, 2001;Catakli et al, 2005).…”

Section: Whig Sco5621 ( = Sav2630)mentioning

confidence: 99%

The evolution of development inStreptomycesanalysed by genome comparisons

2006

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There is considerable information about the genetic control of the processes by which mycelial Streptomyces bacteria form spore-bearing aerial hyphae. The recent acquisition of genome sequences for 16 species of actinobacteria, including two streptomycetes, makes it possible to try to reconstruct the evolution of Streptomyces differentiation by a comparative genomic approach, and to place the results in the context of current views on the evolution of bacteria. Most of the developmental genes evaluated are found only in actinobacteria that form sporulating aerial hyphae, with several being peculiar to streptomycetes. Only four (whiA, whiB, whiD, crgA) are generally present in nondifferentiating actinobacteria, and only two (whiA, whiG) are found in other bacteria, where they are widespread. Thus, the evolution of Streptomyces development has probably involved the stepwise acquisition of laterally transferred DNA, each successive acquisition giving rise either to regulatory changes that affect the conditions under which development is initiated, or to changes in cellular structure or morphology.

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“…The differentiation of Streptomyces cells could be divided into four stages (Figure C): substrate mycelium formation, aerial hypha formation, sporotrichial formation, and spore maturation. WhiAB and WhiGHI are the most important regulatory pathways for the sporulation in Streptomyces , and the gene regulation between these two pathways is mutual. − During the aerial hypha stage, whiA binds to σ whiG (encoded by whiG ) and inhibits the transcription of σ whiG -dependent genes, which hinders the beginning of sporulation. WhiB, encoded by whiB , is also an important regulator, which could combine with WhiA to form a complex WhiAB to activate the expression of key genes required for sporulation, such as bldB , fliP , ftsZ , and ftsK . − In addition, WhiD and SigmaF, indirectly regulated by σ whiG , are two important members of WhiGHI for controlling the probability of spore deformity.…”

Section: Resultsmentioning

confidence: 99%

Establishment of an Efficient Expression and Regulation System in Streptomyces for Economical and High-Level Production of the Natural Blue Pigment Indigoidine

Zhao,

Zhang,

Xiong

et al. 2023

J. Agric. Food Chem.

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Indigoidine, as a kind of natural blue pigment, is widely used in textiles, food, and pharmaceuticals and is mainly synthesized from L-glutamine via a condensation reaction by indigoidine synthetases, most of which originates from Streptomyces species. However, due to the complex metabolic switches of Streptomyces, most of the researchers choose to overexpress indigoidine synthetases in the heterologous host to achieve high-level production of indigoidine. Considering the advantages of low-cost culture medium and simple culture conditions during the large-scale culture of Streptomyces, here, an updated regulation system derived from the Streptomyces self-sustaining system, constructed in our previous study, was established for the highly efficient production of indigoidine in Streptomyces lividans TK24. The updated system was constructed via promoter mining and σ hrdB expression optimization, and this system was applied to precisely and continuously regulate the expression of indigoidine synthetase IndC derived from Streptomyces albus J1704. Finally, the engineered strain was cultured with cheap industrial glycerol as a supplementary carbon source, and 14.3 and 46.27 g/L indigoidine could be achieved in a flask and a 4 L fermentor, respectively, reaching the highest level of microbial synthesis of indigoidine. This study will lay a foundation for the industrial application of Streptomyces cell factories to produce indigoidine.

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Sigma factor WhiG and its regulation constitute a target of a mutational phenomenon occurring during aerial mycelium growth in Streptomyces ambofaciens ATCC23877

Cited by 8 publications

References 36 publications

Two overlapping antiparallel genes encoding the iron regulator DmdR1 and the Adm proteins control sidephore and antibiotic biosynthesis in Streptomyces coelicolor A3(2)

Two overlapping antiparallel genes encoding the iron regulator DmdR1 and the Adm proteins control sidephore and antibiotic biosynthesis in Streptomyces coelicolor A3(2)

The evolution of development inStreptomycesanalysed by genome comparisons

Establishment of an Efficient Expression and Regulation System in Streptomyces for Economical and High-Level Production of the Natural Blue Pigment Indigoidine

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