Signal amplification by enzymatic tools for nucleic acids

Miao, Peng; Tang, Yuguo; Wang, Bidou; Yin, Jian; Ning, Limin

doi:10.1016/j.trac.2014.12.006

Cited by 66 publications

(39 citation statements)

References 50 publications

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“…[9] For example, Plaxco and co-workers reported a methylene blue-labeled supersandwich assay in which each recognition probe can capture multiple target molecules and signal probes upon DNA hybridization. [10] Compared with traditional sandwich assay, such a smart supersandwich assay can amplify the signal remarkably and is very sensitive.…”

Section: Introductionmentioning

confidence: 99%

Highly Sensitive Electrochemiluminescence Assay for Cardiac Troponin I and Adenosine Triphosphate by using Supersandwich Amplification and Bifunctional Aptamer

Liu

et al. 2017

ChemElectroChem

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A highly sensitive electrochemiluminescence (ECL) assay was developed for sequential detection of cardiac troponin I (cTnI) and adenosine triphosphate (ATP) through supersandwich amplification and bifunctional aptamer. The bifunctional aptamer (S1) contained ATP binding aptamer and cTnI binding aptamer. A gold electrode was modified with the specific peptide of cTnI through a self-assembly technique. A sandwichtype conjugate (peptide < cTnI > S1) was formed when the peptide-modified electrode was successively reacted with cTnI and S1. Then, hybridizations between S1 and two ss-DNA auxiliary probes, in which one is complementary with ATP binding aptamer and the other is ATP binding aptamer, led to the formation of long-range ds-DNA on the electrode surface. In the presence of ECL indicator Ru(phen) 3 2 + , a large amount of Ru (phen) 3 2 + was intercalated into ds-DNA grooves, resulting in amplification of the ECL signals. The ECL assay was successfully developed for the detection of cTnI in the range of 8.0 10 À13 to 1.0 10 À11 g/mL based on the increased ECL intensity. After detecting cTnI, ATP was detected in the range of 30 to 500 nM, based on switching structures of aptamers from ds-DNA to ss-DNA/target complex. A low detection limit of 0.3 pg/mL and 10 nM for cTnI and ATP, respectively, was obtained. The employment of a bifunctional aptamer probe and supersandwich signal amplification is promising for the sensitive detection of multiple targets.

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Section: Introductionmentioning

confidence: 99%

Highly Sensitive Electrochemiluminescence Assay for Cardiac Troponin I and Adenosine Triphosphate by using Supersandwich Amplification and Bifunctional Aptamer

Liu

et al. 2017

ChemElectroChem

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“…Some methods involves the techniques like microarrays, PCR and surface plasmon resonance (SPR) may require chemical or enzymatic modications of miRNA prior to the detection. 20 For example, Cheng et al used rolling circle amplication; 21 Miao et al 18,19 Since the level of miRNA is usually much low, many methods employ signal amplication to enhance the sensitivity.…”

Section: Introductionmentioning

confidence: 99%

Retracted Article: Graphene oxide/DNA-decorated electrode for the fabrication of microRNA biosensor

et al. 2015

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“…Nucleases have also been widely applied for effective signal enhancement, owning to their specific catalytic properties in the engineering of DNA structures. [20] For example, Fu et al combined the advantages of high efficiency of exonuclease III (Exo III)-mediated recycling amplification and the high selectivity of designed DNA triplex to target melamine for highly sensitive detection of melamine. [21] Li et al fabricated a nicking endonuclease (NEase)-powered three-dimensional DNA nanomachine for the movement of DNA walker, which was also allowed to be tailored into a DNA nanosensor with isothermal and homogeneous signal amplification.…”

Section: Exonuclease and Nicking Endonuclease-assisted Amplified Elecmentioning

confidence: 99%

Exonuclease and Nicking Endonuclease‐Assisted Amplified Electrochemical Detection of Coralyne

Wang

Zhou

2017

ChemElectroChem

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Based on the catalysis of exonuclease I (Exo I) and nicking endonuclease (NEase), a novel electrochemical biosensor for the detection of coralyne is fabricated in this work. Specifically, DNA probe 1 can be protected from Exo I‐catalyzed digestion upon interacting with coralyne, the most representative homo‐adenine binding molecule. Subsequently, DNA probe 1 can further hybridize with a pendant sequence on top of the DNA tetrahedron, which is previously modified on the gold electrode surface. As the formed duplex contains the recognition site of Nb.BssSI, a type of NEase, the pendant sequence is thus cleaved, releasing the electrochemical probe on the 5’‐terminus from the electrode surface. DNA probe 1 is also released, which could then initiate the subsequent cycles of NEase‐catalyzed cleavage and amplify the declined electrochemical signals. We can determine coralyne in a linear range from 0.1 to 100 nM with the detection limit of 0.07 nM by using the proposed method. Experiments in real samples also demonstrate good results, showing excellent practical utility of this method. Moreover, it could be extended to assay other analytes by modifying certain DNA sequences.

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Signal amplification by enzymatic tools for nucleic acids

Cited by 66 publications

References 50 publications

Highly Sensitive Electrochemiluminescence Assay for Cardiac Troponin I and Adenosine Triphosphate by using Supersandwich Amplification and Bifunctional Aptamer

Highly Sensitive Electrochemiluminescence Assay for Cardiac Troponin I and Adenosine Triphosphate by using Supersandwich Amplification and Bifunctional Aptamer

Retracted Article: Graphene oxide/DNA-decorated electrode for the fabrication of microRNA biosensor

Exonuclease and Nicking Endonuclease‐Assisted Amplified Electrochemical Detection of Coralyne

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