Signal analysis of fiber-optic biosensor for the detection of organophosphorus compounds in the contaminated water

Choi, Jeong‐Woo; Min, Junhong; Lee, Won-Hong

doi:10.1007/bf02706068

Cited by 11 publications

(5 citation statements)

References 11 publications

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“…The control of immobilization of biomolecules onto a desired area is an important technique in various applications such as biosensors, biochips, and microdevices because it can perform high throughput analysis for a short time compared with conventional methods [1][2][3]. However, selective immobilization of biomolecules ("patterning") is difficult to achieve because of nonspecific binding of biomolecules onto fabricated microdevices and the limitation of biomolecules' stability for long-term study.…”

Section: Introductionmentioning

confidence: 99%

Simple fabrication of functionalized surface with polyethylene glycol microstructure and glycidyl methacrylate moiety for the selective immobilization of proteins and cells

Song

Lee

2008

Korean J. Chem. Eng.

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This study reported simple surface modification for the immobilization of biomolecules such as proteins and cells onto desired area at micron-scale level. First, thin film composed of glycidyl methacrylate (GMA) was prepared by UV-photopolymerization. Then, the polyethylene glycol (PEG) microstructures which played a role in the prevention of nonspecific binding of biomolecules were fabricated by using micromolding in capillaries (MIMIC). Thus, we could easily obtain an orthogonal surface having biomolecular attraction and repulsion areas. In addition, we could control of the height of prepared PEG microstructures with spin coating or not. For the investigation of feasibility of biomolecule patterning onto the functionalized surface, FITC-BSA and HEK 293 were examined as representative biomolecule models. A functionalized surface with GMA promotes the strong adhesion of biomolecules, and PEG microstructures located on the background prevent nonspecific binding of biomolecules at micron-scale level. The orthogonal difference in surface functionality showed strong possibility of simple patterning of biomolecules. In addition, the proposed method could easily control the size, shape, and height of patterns. It will be useful platform technology for the construction of a biomolecule array.

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Section: Introductionmentioning

confidence: 99%

Simple fabrication of functionalized surface with polyethylene glycol microstructure and glycidyl methacrylate moiety for the selective immobilization of proteins and cells

Song

Lee

2008

Korean J. Chem. Eng.

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“…PMSF competes with phenylacetate for the active site of AChE, thereby changing K m,1 to an the apparent value K m,1 (Equation ( 5)) [25].…”

Section: Ache Inactivation and Enzyme Kineticsmentioning

confidence: 99%

“…Choi et al developed a mathematical model for a fiber-optic biosensor to detect OPs. This model, which simulated both AChE inhibition kinetics and diffusion, was able to optimize the concentrations of AChE and its substrate [25].…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Biosensor for Markers of Neurological Esterase Inhibition

2021

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A novel, integrated experimental and modeling framework was applied to an inhibition-based bi-enzyme (IBE) electrochemical biosensor to detect acetylcholinesterase (AChE) inhibitors that may trigger neurological diseases. The biosensor was fabricated by co-immobilizing AChE and tyrosinase (Tyr) on the gold working electrode of a screen-printed electrode (SPE) array. The reaction chemistry included a redox-recycle amplification mechanism to improve the biosensor’s current output and sensitivity. A mechanistic mathematical model of the biosensor was used to simulate key diffusion and reaction steps, including diffusion of AChE’s reactant (phenylacetate) and inhibitor, the reaction kinetics of the two enzymes, and electrochemical reaction kinetics at the SPE’s working electrode. The model was validated by showing that it could reproduce a steady-state biosensor current as a function of the inhibitor (PMSF) concentration and unsteady-state dynamics of the biosensor current following the addition of a reactant (phenylacetate) and inhibitor phenylmethylsulfonylfluoride). The model’s utility for characterizing and optimizing biosensor performance was then demonstrated. It was used to calculate the sensitivity of the biosensor’s current output and the redox-recycle amplification factor as a function of experimental variables. It was used to calculate dimensionless Damkohler numbers and current-control coefficients that indicated the degree to which individual diffusion and reaction steps limited the biosensor’s output current. Finally, the model’s utility in designing IBE biosensors and operating conditions that achieve specific performance criteria was discussed.

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“…At the same time, different transduction schemes, such as potentiometric [34][35][36], amperometric [37][38][39][40][41][42][43][44][45], field effect transistors [46,47], optical biosensors [48][49][50][51][52][53][54][55][56][57], fluorescent [58][59][60] and colorimetric indicators [61][62][63][64], have been explored by different research groups worldwide (see review [65][66][67][68]). AChE-based biosensors have a major drawback: since organophoshorus and carbamic pesticides, heavy metals, and detergents exert strong specific inhibition of AChE, they give a sum parameter of AChE inhibition without any qualitative or quantitative information on the individual analytes, i.e.…”

Section: Conventional Strategies For Detection Of Op Pesticidesmentioning

confidence: 99%

Recent Advances in Nanosensors for Organophosphate Pesticide Detection

Liu¹,

Yuan²,

Yue³

et al. 2008

adv powder technol

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Recent trends and challenges in developing nanosensors for the detection of organophosphate (OP) pesticide residues in food are reviewed. Nanosensors have superior properties over the existing techniques such as high-performance liquid chromatography or gas chromatography, because they can provide rapid, sensitive, simple and low-cost on-field detection. The measurement protocols based on nanoparticles and nanotubes are also suitable for mass fabrication of miniaturized devices. The application of nanobiosensors for detection of OP agents is introduced in detail. Future prospects toward the development of selective, sensitive biosensing systems are discussed.

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Signal analysis of fiber-optic biosensor for the detection of organophosphorus compounds in the contaminated water

Cited by 11 publications

References 11 publications

Simple fabrication of functionalized surface with polyethylene glycol microstructure and glycidyl methacrylate moiety for the selective immobilization of proteins and cells

Simple fabrication of functionalized surface with polyethylene glycol microstructure and glycidyl methacrylate moiety for the selective immobilization of proteins and cells

Electrochemical Biosensor for Markers of Neurological Esterase Inhibition

Recent Advances in Nanosensors for Organophosphate Pesticide Detection

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