Signal suppression/enhancement in high-performance liquid chromatography tandem mass spectrometry

Gosetti, Fabio; Mazzucco, Eleonora; Zampieri, Davide; Gennaro, Maria Carla

doi:10.1016/j.chroma.2009.11.060

Cited by 593 publications

(363 citation statements)

References 81 publications

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“…Regarding instrument parameters, DP and CE were optimized so that suitable fragmentation is obtained while the precursor ion remained abundant allowing for MRM analysis. One inherent challenge in the ESI-MS/MS is matrix effect and the inconsistency in ion current response when using ESI source [29]. To account for such variations, the use of internal standard that has similar physicochemical properties to the analyte and elutes at the same time can ensure the validity of the quantitative data [21].…”

Section: Methods Developmentmentioning

confidence: 99%

Development and validation of fast and simple flow injection analysis–tandem mass spectrometry (FIA–MS/MS) for the determination of metformin in dog serum

Michel

Gaunt

Arnason

et al. 2015

Journal of Pharmaceutical and Biomedical Analysis

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A simple, fast and sensitive quantification method for the drug metformin in dog serum was developed using flow injection analysis (FIA)-tandem mass spectrometry (MS/MS). The method was fully validated according to industry standards. It is the first time that FIA-MS/MS for metformin was developed surpassing all existing methods in terms of time of analysis. The quantification method was dependent on the formation of [M+H] + using electrospray ionization (ESI) and employing multiple reaction monitoring (MRM) using quadrupole-linear ion trap (4000 QTRAP ® ) instrument. A deuterated internal standard (IS) of metformin bearing six deuterium atoms was used to compensate for matrix effects and for variation in ion current within the ESI source. The ion transitions that were monitored were m/z 130.1 m/z 71.0 and m/z 130.1 m/z 60.1 for metformin and m/z 136.0 m/z 77.0 for the internal standard. A linear response (r = 0.9966) was established for a range of concentrations of 5-2340 ng/ml. The inter-and intra-day variations were within the acceptable criteria for all quality control samples. The method was successfully applied for measurement of serum metformin concentration in dogs after intravenous injection.

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Section: Methods Developmentmentioning

confidence: 99%

Development and validation of fast and simple flow injection analysis–tandem mass spectrometry (FIA–MS/MS) for the determination of metformin in dog serum

Michel

Gaunt

Arnason

et al. 2015

Journal of Pharmaceutical and Biomedical Analysis

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“…The matrix effect was also evaluated in a second way using post-column injection 25,12,26 . A syringe pump was connected to the HPLC column effluent using a tee.…”

Section: Lc-ms/ms Analysismentioning

confidence: 99%

“…However, an important feature is the clean-up of samples prior to analysis. The presence of matrix interferences leads to ion suppression or enhancement in many cases 11,12 . In this investigation a molecularly imprinted polymer (MIP) 13,14,15,16 was developed and applied as a sorbent in a molecularly imprinted solid phase extraction (MISPE) 17,18 protocol.…”

Section: Introductionmentioning

confidence: 99%

Development of Suspension Polymerized Molecularly Imprinted Beads with Metergoline as Template and Application in a Solid-Phase Extraction Procedure toward Ergot Alkaloids

Lenain

Mavungu²,

Dubruel³

et al. 2012

Anal. Chem.

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The first successfully developed molecularly imprinted polymer toward six ergot alkaloids and their respective epimers is described. A new imprinting molecule, metergoline, was used as template analogue in the production of suspension polymerized beads. These spherical particles functioned as selective sorbent in a solid-phase extraction column. The application of this column in the cleanup of barley samples prior to liquid chromatography coupled with tandem mass spectrometry allowed simple and cost-efficient sample preparation. The performance of the imprinted polymer and a non-imprinted control polymer was evaluated. This includes determination of the recovery values and the matrix effect of each of the 12 tested ergot alkaloids as well as a crossreactivity study with 25 common mycotoxins. The binding isotherms were obtained for metergoline, thus allowing comparison with other (imprinted) sorbents. A comparison between bulk and suspension polymerization is provided to determine the appropriate production technique. E rgot alkaloids are mycotoxins produced by Claviceps species, specifically Claviceps purpurea. These fungi can be found on cereals and produce sclerotia, which vary in composition and concentration of ergot alkaloids. The sclerotia are harvested together with the grains and only up to 80% of the kernels can be removed with the mechanical separation techniques currently available. Especially in periods of drought these techniques become unreliable as smaller sclerotia, similar in size to the grains, are produced. Generally, sclerotia are also broken during transport, allowing them to enter the food chain more easily.These ergot alkaloids possess toxicity due to interactions with α-adrenergic, serotinergic, and dopaminergic receptors. 1−3 Excess intake by humans can cause nausea, convulsions, hallucinations, and vasoconstriction and can even lead to gangrenous symptoms and abortion. Animals are infected through consumption of contaminated feed. This is a widespread phenomenon in livestock leading to adverse effects ranging from weight loss to death of infected animals. [4][5][6]2,7 The European Food Safety Authority (EFSA) stated in 2005 that the degree of variability in ergot alkaloid pattern in relation to fungal species, host plant, and geographical distribution is not known. 5 Six ergot alkaloidsergometrine, ergosine, ergotamine, ergocristine, ergocryptine, and ergocornine which are considered the most important ones according to EFSA, are depicted in Figure 1. An isomerization from the biologically active R-form to the biologically inactive S-form occurs at the C 8 stereogenic center of the tetracyclic ergoline structure in a reversible epimerization reaction. 8,9,6 The suffix "-ine" is used in the nomenclature if the side chain is present in the R-form at the C 8 stereogenic center, for example, ergotamine. The side chain is present in the S-orientation in the corresponding epimer which is expressed by the suffix "-inine", for example, ergotaminine. This reaction is promoted in humid, aqueous,...

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“…3 For example, matrix compounds entering the ionization at the same time with caffeine derivative. 41 In addition, other pharmaceuticals could exist in the samples after SPE treatment. 4 Referring to the work of Sui et al, 2,35 the matrix suppression of caffeine in wastewater was 49%, and a calibration curve was applied for the quantitation of caffeine by a standard solution.…”

Section: Methods Validationmentioning

confidence: 99%

Determination of Caffeine and Its Metabolites in Wastewater Treatment Plants Using Solid-Phase Extraction and Liquid Chromatography–Tandem Mass Spectrometry

Echigo

Itoh

2018

ANAL. SCI.

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For caffeine and its seven major metabolites (i.e., theobromine, theophylline, paraxanthine, 1-methylxanthine, 3-methylxanthine, 7-methylxanthine, and xanthine), an optimized analytical method using liquid chromatography-tandem mass spectrometry (LC-MS/MS) for their detection in wastewater samples was developed in this study. Extraction of these compounds (recoveries ranged from 60.3 to 83.2%) was made possible by combining universal polymeric reversedphase cartridge and polymeric strong cation exchange cartridge. This method was applied to the determination of caffeine and its metabolites in the influent and effluent of an anaerobic-anoxic-oxic (A2O) process. In the A2O influent, caffeine and its metabolites (except xanthine) ranged from 1.39 to 5.45 μg/L, and their concentrations in the A2O effluent ranged from 10.2 to 171.3 ng/L. The mass load of caffeine was 14.9 g/day/1000 inhabitants, considering the population served by the target wastewater treatment plant (WWTP). The concentration of caffeine derivatives in wastewater influent is a tool for estimating the population size in the area served by WWTPs.

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Signal suppression/enhancement in high-performance liquid chromatography tandem mass spectrometry

Cited by 593 publications

References 81 publications

Development and validation of fast and simple flow injection analysis–tandem mass spectrometry (FIA–MS/MS) for the determination of metformin in dog serum

Development and validation of fast and simple flow injection analysis–tandem mass spectrometry (FIA–MS/MS) for the determination of metformin in dog serum

Development of Suspension Polymerized Molecularly Imprinted Beads with Metergoline as Template and Application in a Solid-Phase Extraction Procedure toward Ergot Alkaloids

Determination of Caffeine and Its Metabolites in Wastewater Treatment Plants Using Solid-Phase Extraction and Liquid Chromatography–Tandem Mass Spectrometry

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