Signal transduction in electrically stimulated articular chondrocytes involves translocation of extracellular calcium through voltage-gated channels

Xu, Jihong; Wang, W.; Clark, Charles C.; Brighton, Carl T.

doi:10.1016/j.joca.2008.07.001

Cited by 101 publications

(122 citation statements)

References 62 publications

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“…The genes that showed the most significant elevations at that time were Nav1.5 and SCN7a, which both code for voltage gated sodium channels. There was a strong correlation between elevated expression levels of signal transduction pathways and voltage gated ion channels and ES, and our results supported those findings [16]. The upregulation of CX45, a cardiac specific gap junction protein, was also significantly evident on day 6, and the histological assessment reinforces the translation of the CX45 sequences into functional protein.…”

Section: Discussionsupporting

confidence: 90%

Electrical Stimulation of Artificial Heart Muscle: A Look Into the Electrophysiologic and Genetic Implications

Mohamed

Islas

Schwartz³

et al. 2017

ASAIO Journal

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Development of tissue-engineered hearts for treatment of myocardial infarction or biological pacemakers has been hindered by the production of mostly arrhythmic or in-synergistic constructs. Electrical stimulation (ES) of these constructs has been shown to produce tissues with greater twitch force and better adrenergic response. In order to further our understanding of the mechanisms underlying the effect of ES, we fabricated a bioreactor capable of delivering continuous or intermittent waveforms of various types to multiple constructs simultaneously. In this study, we examined the effect of an intermittent biphasic square wave on our artificial heart muscle (AHM) composed of neonatal rat cardiac cells and fibrin gel. Twitch forces, spontaneous contraction rates, biopotentials, gene expression profiles, and histological observations were examined for the ES protocol over a 12 day culture period. We demonstrate improved consistency between samples for twitch force and contraction rate, and higher normalized twitch force amplitudes for electrically stimulated AHM. Improvements in electrophysiology within the AHM was noted by higher conduction velocities and lower latency in electrical response for electrically stimulated AHM. Genes expressing key electrophysiological and structural markers peaked at days 6 and 8 of culture, only a few days after the initiation of ES. These results may be used for optimization strategies to establish protocols for producing AHM capable of replacing damaged heart tissue in either a contractile or electrophysiological capacity. Optimized AHM can lead to alternative treatments to heart failure and alleviate the limited donor supply crisis.

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Section: Discussionsupporting

confidence: 90%

Electrical Stimulation of Artificial Heart Muscle: A Look Into the Electrophysiologic and Genetic Implications

Mohamed

Islas

Schwartz³

et al. 2017

ASAIO Journal

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“…Field strength was comparable to prior studies utilizing electric fields for bone differentiation, cartilage repair and neural tissue engineering. 19,24,61,62 …”

Section: Resultsmentioning

confidence: 99%

Neural responses to electrical stimulation on patterned silk films

Hronik-Tupaj

Raja

Tang-Schomer

et al. 2013

J Biomedical Materials Res

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Peripheral nerve injury is a critical issue for trauma patients. Following injury, incomplete axon regeneration or misguided axon innervation into tissue will result in loss of sensory and motor functions. The objective of this study was to examine axon outgrowth and axon alignment in response to surface patterning and electrical stimulation. To accomplish our objective, metal electrodes with dimensions of 1.5 mm × 4 cm, were sputter coated onto micropatterned silk protein films, with surface grooves 3.5 μm wide × 500 nm deep. P19 neurons were seeded on the patterned electronic silk films and stimulated at 120 mV, 1 kHz, for 45 minutes each day for 7 days. Responses were compared to neurons on flat electronic silk films, patterned silk films without stimulation, and flat silk films without stimulation. Significant alignment was found on the patterned film groups compared to the flat film groups. Axon outgrowth was greater (p < 0.05) on electronic films on day 5 and day 7 compared to the unstimulated groups. In conclusion, electrical stimulation, at 120 mV, 1 kHz, for 45 minutes daily, in addition to surface patterning, of 3.5 μm wide × 500 nm deep grooves, offered control of nerve axon outgrowth and alignment.

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“…However, the direct targets of EMFs on the cellular surface have been unknown. There has been substantial evidence to support that voltage-gated calcium channels (VGCCs) are such a direct target (Hojevik et al, 1995;Kindzelskii and Petty, 2005;Morgado et al, 1998;Piacentini et al, 2008;Xu et al, 2009) The Alizarin red staining results after 12 days and the comparison for the numbers and areas of mineralized nodules. *P < 0.05 or **P < 0.01 vs Nc, ▲ P < 0.05 or ▲▲ P < 0.01 vs P + siRNA.…”

Section: Discussionmentioning

confidence: 99%

Pulsed electromagnetic fields promote osteoblast mineralization and maturation needing the existence of primary cilia

Yan

Zhou

et al. 2015

Molecular and Cellular Endocrinology

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Although pulsed electromagnetic fields (PEMFs) have been approved as a therapy for osteoporosis, action mechanisms and optimal parameters are elusive. To determine the optimal intensity, exposure effects of 50 Hz PEMFs of 0.6-3.6 mT (0.6 interval at 90 min/day) were investigated on proliferation and osteogenic differentiation of cultured calvarial osteoblasts. All intensity groups stimulated proliferation significantly with the highest effect at 0.6 mT. The 0.6 mT group also obtained the optimal osteogenic effect as demonstrated by the highest ALP activity, ALP(+) CFU-f colony formation, nodule mineralization, and expression of COL-1 and BMP-2. To verify our hypothesis that the primary cilia are the cellular sensors for PEMFs, osteoblasts were also transfected with IFT88 siRNA or scrambled control, and osteogenesis-promoting effects of 0.6 mT PEMFs were found abrogated when primary cilia were inhibited by IFT88 siRNA. Thus primary cilia of osteoblasts play an indispensable role in mediating PEMF osteogenic effect in vitro.

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Signal transduction in electrically stimulated articular chondrocytes involves translocation of extracellular calcium through voltage-gated channels

Cited by 101 publications

References 62 publications

Electrical Stimulation of Artificial Heart Muscle: A Look Into the Electrophysiologic and Genetic Implications

Electrical Stimulation of Artificial Heart Muscle: A Look Into the Electrophysiologic and Genetic Implications

Neural responses to electrical stimulation on patterned silk films

Pulsed electromagnetic fields promote osteoblast mineralization and maturation needing the existence of primary cilia

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