Signal transduction to mitochondrial ATP synthase: Evidence that PDGF-dependent phosphorylation of the δ-subunit occurs in several cell lines, involves tyrosine, and is modulated by lysophosphatidic acid

Ko, Young Hee; Pan, Weiying; Inoue, Chiyoko N.; Pedersen, Peter L.

doi:10.1016/s1567-7249(01)00036-8

Cited by 41 publications

(28 citation statements)

References 32 publications

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“…Thus, there is a possibility that a cAMP-dependent phosphorylation may be involved in regulation of a function of the 45S complex and that it is modulated by the LmjF32.0650 protein. It was shown that cAMPdependent pathways are involved in regulation of oxidative phosphorylation complexes in other mitochondria [49][50][51].…”

Section: Discussionmentioning

confidence: 99%

Proteomics and electron microscopic characterization of the unusual mitochondrial ribosome-related 45S complex in Leishmania tarentolae

Maslov

Spremulli

Sharma

et al. 2007

Molecular and Biochemical Parasitology

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A novel type of ribonucleoprotein (RNP) complex has been described from the kinetoplastmitochondria of Leishmania tarentolae. The complex, termed the 45S SSU*, contains the 9S small subunit rRNA but does not contain the 12S large subunit rRNA. This complex is the most stable and abundant mitochondrial RNP complex present in Leishmania. As shown by tandem mass spectrometry, the complex contains at least 39 polypeptides with a combined molecular mass of almost 2.1 MDa. These components include several homologs of small subunit ribosomal proteins (S5, S6, S8 S9, S11, S15, S16, S17, S18, MRPS29); however, most of the polypeptides present are unique. Only a few of them show recognizable motifs, such as protein-protein (coiled-coil, Rhodanese) or protein-RNA (pentatricopeptide repeat) interaction domains. A cryo-electron microscopy examination of the 45S SSU* fraction reveals that 27% of particles represent SSU homodimers arranged in a head-to-tail orientation, while the majority of particles are clearly different and show an asymmetric bilobed morphology. Multiple classes of two-dimensional averages were derived for the asymmetrical particles, probably reflecting random orientations of the particles and difficulties in correlating these views with the known projections of ribosomal complexes. One class of the two-dimensional averages shows an SSU moiety attached to a protein mass or masses in a monosome-like appearance. The combined mass spectrometry and electron microscopy data thus indicate that the majority 45S SSU* particles represents a heterodimeric complex in which the SSU Publisher's Disclaimer: This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. NIH Public Access

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Section: Discussionmentioning

confidence: 99%

Proteomics and electron microscopic characterization of the unusual mitochondrial ribosome-related 45S complex in Leishmania tarentolae

Maslov

Spremulli

Sharma

et al. 2007

Molecular and Biochemical Parasitology

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“…Several reports document the phosphorylation of the ␣-and ␥-subunits (14,19,20), whereas Zhang et al (8) report phosphorylation of tyrosine 75 on the ␦-subunit of the F 1 domain of F 0 F 1 -ATPase in response to platelet-derived growth factor (PDGF) stimulation in cortical neurons. PDGF-mediated tyrosine phosphorylation of the ␦-subunit of ATP synthase has also been reported in other cell types (9). F 0 F 1 -ATPase associates and interacts with protein kinase C ␦ (PKC-␦) in cardiac myocytes and with PKC-␤ in the hippocampus after transient ischemia (21,22).…”

mentioning

confidence: 85%

Protein Kinase C-α Interaction with F0F1-ATPase Promotes F0F1-ATPase Activity and Reduces Energy Deficits in Injured Renal Cells

Nowak

Bakajsova

2015

Journal of Biological Chemistry

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“…4A and B) could be due to decreased glycolysis or decreased mitochondrial function (27,31). To characterize the contribution of these mechanisms to decreased ATP, we measured mitochondrial membrane potential before and after hPDGFB inhibition.…”

Section: Pdgf Regulates Glucose Metabolism In Glioma-derived Tscsmentioning

confidence: 99%

Proliferation-Independent Control of Tumor Glycolysis by PDGFR-Mediated AKT Activation

Ran¹,

Liu²,

Hitoshi³

et al. 2013

Cancer Research

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The differences in glucose metabolism that distinguish most malignant and normal tissues have called attention to the importance of understanding the molecular mechanisms by which tumor energy metabolism is regulated. Receptor tyrosine kinase (RTK) pathways that are implicated in proliferation and transformation have been linked to several aspects of tumor glucose metabolism. However, the regulation of glycolysis has invariably been examined under conditions in which proliferation is concomitantly altered. To determine whether RTKs directly regulate glycolysis without prerequisite growth modulation, we first identified a specific RTK signaling pathway, platelet-derived growth factor (PDGF)/PDGF receptor (PDGFR) that regulates glycolysis in glioma-derived tumor stem-like cells from a novel mouse model. We determined that PDGFregulated glycolysis occurs independent of PDGF-regulated proliferation but requires the activation of AKT, a known metabolic regulator in tumor. Our findings identifying a key characteristic of brain tumors, aerobic glycolysis, mediated by a pathway with multiple therapeutic targets suggests the possibility of inhibiting tumor energy metabolism while also treating with agents that target other pathways of pathologic significance.

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Signal transduction to mitochondrial ATP synthase: Evidence that PDGF-dependent phosphorylation of the δ-subunit occurs in several cell lines, involves tyrosine, and is modulated by lysophosphatidic acid

Cited by 41 publications

References 32 publications

Proteomics and electron microscopic characterization of the unusual mitochondrial ribosome-related 45S complex in Leishmania tarentolae

Proteomics and electron microscopic characterization of the unusual mitochondrial ribosome-related 45S complex in Leishmania tarentolae

Protein Kinase C-α Interaction with F0F1-ATPase Promotes F0F1-ATPase Activity and Reduces Energy Deficits in Injured Renal Cells

Proliferation-Independent Control of Tumor Glycolysis by PDGFR-Mediated AKT Activation

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