Signaling components involved in Bcl-w-induced migration of gastric cancer cells

Bae, In Hwa; Yoon, Sung Hwan; Lee, Seung Bum; Park, Jong Kuk; Ho, Jin‐Nyoung; Um, Hong‐Duck

doi:10.1016/j.canlet.2008.11.022

Cited by 29 publications

(33 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The mechanism by which Bcl-w suppresses apoptosis is that Bcl-w interacts directly with pro-apoptotic members to block their apoptotic activities (Gross et al , 1999; Antonsson, 2001; Uren et al , 2007; Bae et al , 2009). Recent evidence suggests that the expression of Bcl-w is significantly associated with infiltrative morphotypes (Lee et al , 2003).…”

Section: Discussionmentioning

confidence: 99%

MicroRNA-335 acts as a metastasis suppressor in gastric cancer by targeting Bcl-w and specificity protein 1

et al. 2011

View full text Add to dashboard Cite

Aberrant expression of miR-335 has been frequently reported in cancer studies, suggesting that there is a close correlation between miR-335 and cancer during its development, progression, metastasis and prognosis. The expression of miR-335 in gastric cancer and its effects are not known. Relative expression of miR-335 in 4 gastric cancer cell lines and in 70 gastric cancer tissues was confirmed by real-time quantitative reverse transcriptase-PCR compared with controls. Transwell cell migration and Matrigel invasion assay in vitro and metastasis formation assay in vivo were used to examine the effects of miR-335 expression on gastric cancer cell invasion and metastasis. The effect of miR-335 expression on gastric cancer cell proliferation was estimated by the 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide assay. Luciferase reporter assay and western blot were used to examine the potential target genes and related pathways. Gene silencing with small-interfering RNA was used to examine the effects of target genes on gastric cancer cell invasion. miR-335 was dramatically downregulated in gastric cancer cell lines than in the normal gastric cell line GES-1. Low expression of miR-335 was significantly associated with lymph-node metastasis, poor pT stage, poor pN stage and invasion of lymphatic vessels. Overexpression of miR-335 suppressed gastric cancer cell invasion and metastasis in vitro and in vivo, but has no significant effects on cell proliferation. Furthermore, miR-335 might suppress gastric cancer invasion and metastasis by targeting Bcl-w and specificity protein 1 (SP1). Taken together, our results provide evidence that miR-335 might function as a metastasis suppressor in gastric cancer by targeting SP1 directly and indirectly through the Bcl-w-induced phosphoinositide 3-kinase-Akt-Sp1 pathway. miR-335 showing altered expression at different stages of gastric cancer could be a target for gastric cancer therapies and could be further developed as a potential prognostic factor.

show abstract

Section: Discussionmentioning

confidence: 99%

MicroRNA-335 acts as a metastasis suppressor in gastric cancer by targeting Bcl-w and specificity protein 1

et al. 2011

View full text Add to dashboard Cite

show abstract

“…The role of MMP-2 and MMP-9 has been confirmed in colorectal cancer (3)(4)(5) and gastric cancer (6)(7)(8). Increased levels of MMP-2 and MMP-9 in patients with colorectal and gastric cancer were detected in serum (9,10), plasma (3,9), and tissue (3,11,12).…”

Section: Introductionmentioning

confidence: 88%

Serum levels of matrix metalloproteinase-2 and -9 and conventional tumor markers (CEA and CA 19-9) in patients with colorectal and gastric cancers

Emara

Cheung

Grabowski

et al. 2009

Clinical Chemistry and Laboratory Medicine

View full text Add to dashboard Cite

Background: Matrix metalloproteinases (MMPs), especially MMP-2 and MMP-9, play an important role in tumor invasion and metastasis. This study aimed to determine the serum levels of MMP-2, MMP-9, 130-and 225-kDa gelatinolytic bands and conventional tumor markers, carcinoembryonic antigen (CEA) and cancer antigen (CA) 19-9, in patients with gastrointestinal cancers. The relationship between these parameters and clinicopathological factors was also studied. Methods: Sera from controls (ns19), and patients with colorectal (ns47) and gastric (ns34) cancer were collected prospectively. The gelatinolytic activities of MMP-2, MMP-9, 130-and 225-kDa bands were determined using gelatin zymography. CEA and CA 19-9 were determined using immunoradiometric assay (IRMA). Results: Serum levels of MMP-9, 130-and 225-kDa gelatinolytic bands, CEA, and CA 19-9, but not MMP-2, in colorectal and gastric cancer were significantly higher than that of controls. No significant correlation was found between histological grade or clinical stage and levels of MMP-9, 130-and 225-kDa gelatinolytic bands, which were correlated (rs0.61-0.89, p-0.005). Conclusions: Our findings suggest that zymographic determination of

show abstract

“…Because PI3K and Akt act upstream of Sp1, 22 and the transcription activity of the human Col1A1 could be positively regulated by Sp1, 23 modulation of the level or of the transcriptional activity of Sp1 might play an important role in the regulation of collagen gene expression. In addition, according Effect of miR-29b on TGF␤1-mediated Akt phosphorylation.…”

Section: Mir-29b Suppresses Tgf␤1-mediated Activation Of Col1a1 Gene mentioning

confidence: 99%

Suppression of Type I Collagen Expression by miR-29b via PI3K, Akt, and Sp1 Pathway in Human Tenon's Fibroblasts

Cui

Duan

et al. 2012

Invest. Ophthalmol. Vis. Sci.

View full text Add to dashboard Cite

In all, 38 miRNAs were identified to be upregulated and 31 downregulated, in TGFβ1-stimulated HTFs. Among those, the miR-29b, downregulated in TGFβ1-treated HTFs, targeted a cadre of mRNAs that encode proteins involved in fibrosis, including PI3Kp85α, Sp1, and collagen type I alpha1 (Col1A1). Treatment of HTFs with TGFβ1 activated the PI3K/Akt/Sp1 pathway and, consequently, induced an increase in the expression of type I collagen. Overexpression of miR-29b inhibited the PI3K/Akt/Sp1 pathway, and attenuated the expression of Col1A1. CONCLUSIONS. miR-29b acted as a suppressor of type I collagen gene by repressing the PI3K/Akt/Sp1 pathway in HTFs. Overexpression of miR-29b protected subconjunctival tissues against collagen production and fibrosis. These findings provided a novel rationale for the development of miRNA-based strategies for attenuating scar formation after glaucoma filtering surgery.

show abstract

Signaling components involved in Bcl-w-induced migration of gastric cancer cells

Cited by 29 publications

References 20 publications

MicroRNA-335 acts as a metastasis suppressor in gastric cancer by targeting Bcl-w and specificity protein 1

MicroRNA-335 acts as a metastasis suppressor in gastric cancer by targeting Bcl-w and specificity protein 1

Serum levels of matrix metalloproteinase-2 and -9 and conventional tumor markers (CEA and CA 19-9) in patients with colorectal and gastric cancers

Suppression of Type I Collagen Expression by miR-29b via PI3K, Akt, and Sp1 Pathway in Human Tenon's Fibroblasts

Contact Info

Product

Resources

About