Signaling differences in peripheral blood mononuclear cells of high and low vaccine responders prior to, and following, vaccination in piglets

Lipsit, Sean; Facciuolo, Antonio; Scuten, Erin; Wilkinson, James; Plastow, Graham; Kusalik, Anthony; Napper, Scott

doi:10.1016/j.jvacx.2022.100167

Cited by 1 publication

(1 citation statement)

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“…In addition to stimulating humoral responses, the induction of cellular immunity is also critical for providing protection [ 40 ]. It is noted that the commercial vaccine was observed to elicit a high level of IgG and IgM antibodies in mice that could recognize native proteins of M. hyopneumoniae , while failing to stimulate IFN-γ gene expression.…”

Section: Discussionmentioning

confidence: 99%

A Recombinant Chimera Vaccine Composed of LTB and Mycoplasma hyopneumoniae Antigens P97R1, mhp390 and P46 Elicits Cellular Immunologic Response in Mice

et al. 2023

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Mycoplasma hyopneumoniae is the etiological agent of porcine enzootic pneumonia (EP), leading to a mild and chronic pneumonia in swine. Relative control has been attained through active vaccination programs, but porcine enzootic pneumonia remains a significant economic challenge in the swine industry. Cellular immunity plays a key role in the prevention and control of porcine enzootic pneumonia. Therefore, the development of a more efficient vaccine that confers a strong immunity against M. hyopneumoniae is necessary. In this study, a multi-antigen chimera (L9m6) was constructed by combining the heat-labile enterotoxin B subunit (LTB) with three antigens of M. hyopneumoniae (P97R1, mhp390, and P46), and its immunogenic and antigenic properties were assessed in a murine model. In addition, we compared the effect of individual administration and multiple-fusion of these antigens. The chimeric multi-fusion vaccine induced significant cellular immune responses and high production of IgG and IgM antibodies against M. hyopneumoniae. Collectively, our data suggested that rL9m6 chimera exhibits potential as a viable vaccine candidate for the prevention and control of porcine enzootic pneumonia.

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Section: Discussionmentioning

confidence: 99%