Signature of genome wide gene expression in classical swine fever virus infected macrophages and PBMCs of indigenous vis-a-vis crossbred pigs

Sah, V. K.; Kumar, Amit; Dhar, P.; Upmanyu, Vikramaditya; Tiwari, Ashok Kumar; Wani, Sajad A.; Sahu, Anamika; Kumar, Ajay; Badasara, Surendra Kumar; Pandey, Awadh Bihari; Saxena, Shikha; Rai, Anil; Mishra, Bishnu Prasad; Singh, Raj Kumar; Gandham, Ravi Kumar

doi:10.1016/j.gene.2020.144356

Cited by 6 publications

(4 citation statements)

References 38 publications

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“…Previously, Sah et al . (2020) also found more genes to be upregulated in Ghurrah pigs at day 7 after CSF vaccination compared to Ghurrah x Landrace crossbreds pig, suggesting that the immune system of the indigenous breed is in a more activated state at day 7 after vaccination.…”

Section: Discussionmentioning

confidence: 93%

“…Previous work in our lab has showed a significant difference between the CSF E2 antibody levels measured 5 weeks after vaccination in indigenous Ghurrah breed of India, and Ghurrah x Landrace crossbreds (Singh, Kumar, et al 2016). Follow-up transcriptomics studies revealed the differences between the peripheral blood mononuclear cell (PBMC) transcriptome between these two genetic groups at days 7 and 28 after CSF vaccination (Pathak et al 2017;Sah et al 2020). However, these studies had a constraint of only one representative sample per breed at each time point, thus providing only limited resolution of changes in the gene expression.…”

Section: Introductionmentioning

confidence: 99%

“…2017; Sah et al . 2020). However, these studies had a constraint of only one representative sample per breed at each time point, thus providing only limited resolution of changes in the gene expression.…”

Section: Introductionmentioning

confidence: 99%

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Characterisation and comparison of immune response mechanisms in an indigenous and a commercial pig breed after classical swine fever vaccination

et al. 2021

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The live attenuated classical swine fever (CSF) vaccine has been successfully used to prevent and control CSF outbreaks for 6 decades. However, the immune response mechanisms against the vaccine remain poorly understood. Moreover, very few reports exist regarding the breed differences in the response to CSF vaccine. In this study, we generated the peripheral blood mononuclear cell transcriptomes of indigenous Ghurrah and commercial Landrace pig breeds, before and 7 days after CSF vaccination. Subsequently, between and within-breed differential gene expression analyses were carried out. Results revealed large differences in pre-vaccination peripheral blood mononuclear cell transcriptome profiles of the two breeds, which were homogenised 7 days after vaccination. Before vaccination, gene set enrichment analysis showed that pathways related to antigen sensing and innate immune response were enriched in Ghurrah, while pathways related to adaptive immunity were enriched in Landrace. Ghurrah exhibited greater immunomodulation compared to Landrace following the vaccination. In Ghurrah, cell-cycle processes and T-cell response pathways were upregulated after vaccination. However, no pathways were upregulated in Landrace after vaccination. Pathways related to inflammation were downregulated in both the breeds after vaccination. Key regulators of inflammation such as IL1A, IL1B, NFKBIA and TNF genes were strongly downregulated in both the breeds after vaccination. Overall, our results have elucidated the mechanisms of host immune response against CSF vaccination in two distinct breeds and revealed common key genes instrumental in the global immune response to the vaccine.

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Section: Discussionmentioning

confidence: 93%

Section: Introductionmentioning

confidence: 99%

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Characterisation and comparison of immune response mechanisms in an indigenous and a commercial pig breed after classical swine fever vaccination

et al. 2021

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“…This suggests that IFIT1 is important in antiviral replication. Furthermore, Vaishali Sah et al used IFIT1 as a key indicator of immunity by measuring its expression level after swine fever vaccination ( 51 ). It has been reported that interferon can significantly trigger the production of many interferon-induced genes (IFIT1, IFITM3, MX-1, OASL, ISG15, PKR, GBP1, Viperin, BST2, IRF-1, and CXCL10), which play key roles in the resistance to viral infection ( 52 ).…”

Section: Discussionmentioning

confidence: 99%

Integrated analysis of lncRNA-mediated ceRNA network involved in immune regulation in the spleen of Meishan piglets

Shi

Xu²,

Wu³

et al. 2022

Front. Vet. Sci.

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Meishan pigs are a famous local pig breed in China, with high fertility and early sexual maturity, and stronger immunity compared to other breeds. The spleen is the largest lymphoid organ in pigs and performs essential functions, such as those relating to immunity and haematopoiesis. The invasion of many pathogenic microorganisms in pigs is associated with spleen damage. Long non-coding RNAs participate in a broad range of biological processes and have been demonstrated to be associated with splenic immune regulation. However, the expression network of mRNAs and lncRNAs in the spleen of Meishan pigs remains unclear. This study collected spleen tissues from Meishan piglets at three different ages as a model, and mRNA and lncRNA transcripts were profiled for each sample. Additionally, 1,806 differential mRNAs and 319 differential lncRNAs were identified. A complicated interaction between mRNAs and lncRNAs was identified via WGCNA, demonstrating that lncRNAs are a crucial regulatory component in mRNA. The results show that the modules black and red have similar mRNA and lncRNA transcription patterns and are mainly involved in the process of the immune defense response. The core genes (DHX58 and IFIT1) and key lncRNAs (TCONS-00002102 and TCONS-00012474) of piglet spleen tissue were screened using the ceRNA network. The expression of these genes is related to the immune response of pigs. Our research may contribute to a further understanding of mRNA and lncRNA expression in the spleen of piglets, and provide new ideas to improve the disease resistance of piglets.

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Selection and validation of differentially expressed metabolic and immune genes in weaned Ghurrah versus crossbred piglets

Karthikeyan¹,

Pathak²,

Kumar³

et al. 2020

Trop Anim Health Prod

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Signature of genome wide gene expression in classical swine fever virus infected macrophages and PBMCs of indigenous vis-a-vis crossbred pigs

Cited by 6 publications

References 38 publications

Characterisation and comparison of immune response mechanisms in an indigenous and a commercial pig breed after classical swine fever vaccination

Characterisation and comparison of immune response mechanisms in an indigenous and a commercial pig breed after classical swine fever vaccination

Integrated analysis of lncRNA-mediated ceRNA network involved in immune regulation in the spleen of Meishan piglets

Selection and validation of differentially expressed metabolic and immune genes in weaned Ghurrah versus crossbred piglets

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