Significance of Expression of Cyclin D as an Early Indicator in Dysplastic Transformation of Oral Mucosa in Tobacco Users

Batool, Sarwat; Fahim, Amin; Qureshi, Anila; Anas, Sumayya; Qamar, Nazia; Shahid, Kamran

doi:10.25301/jpda.284.171

Cited by 3 publications

(3 citation statements)

References 19 publications

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“…The increased expression of COX-2 during oral carcinogenesis may depend on the developmental stage of the tumor, as well as etiologic factors such as the types of mutations and distinct types of injuries affecting different regions [22]. Few researchers reported prevalence rates of the cyclin D1 overexpression range from low-grade oral epithelial dysplasia to high-grade in all degrees of oral epithelial dysplasia [23] Similar results were observed in other studies with significant increase in the expression from mild to moderate to severe dysplasia [24,25].…”

Section: Discussionmentioning

confidence: 99%

The expression of Cox-2 and Cyclin D1 with clinicopathological characteristics in oral epithelial dysplasia: A retrospective study

Mustafa Mohammed Abdulhussain

2023

World J. Biol. Pharm. Res.

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Background: Oral mucosal cancers are often preceded by potentially malignant diseases that may exhibit diverse morphological and histological alterations. The expression of COX 2 has been related to tumor vascularization, dissemination, and survival in oral cancer studies. High levels of cyclin D1 expression to precancerous lesions and oral malignancies. This study was designed to evaluate and compare the immunohistochemical expression of the above-mentioned markers. Materials and Methods: 50 retrospective formalin fixed-paraffin embedded histopathologically confirmed blocks of oral epithelial dysplasia. Immunohistochemical staining was performed using monoclonal antibodies against COX-2 and Cyclin D1. Using a standard camera, photomicrographs of the slides were taken. Results: No significant difference in the age distribution of the patients studied, as well as age pivoting at the fifth and sixth decades with mean and standard deviation (53.36 2.63). There was no significant difference in gender among the studied patients. Furthermore, there was a highly significant difference in grade distribution. Conclusions: The COX-2 and Cyclin D1 markers may assist in diagnosing oral epithelial dysplasia based on severity. It's easy to gather image-analytic data on the stained tissue specimens.

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Section: Discussionmentioning

confidence: 99%

The expression of Cox-2 and Cyclin D1 with clinicopathological characteristics in oral epithelial dysplasia: A retrospective study

Mustafa Mohammed Abdulhussain

2023

World J. Biol. Pharm. Res.

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“…Cyclin D1 is a proto-oncogene that is encoded by the CCND1 gene and is located on chromosome 11q13. It is a part of the molecular system that observes and controls the cell cycle during the transition from the G1 to the S phase of the cell cycle (Basnaker et al, 2014;Batool et al, 2019). Cyclin D1 is the first cyclin to accumulate throughout the cell cycle; it rises during the G1 phase but is not detected during the S phase.…”

Section: Introductionmentioning

confidence: 99%

Immunohistochemical Expression of Cyclin D1 in Oral Lichen Planus

Abdulhussain¹,

Alaswad²

2022

jhms

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Background: Many studies have reported that a condition initially classified as oral lichen planus (OLP) has a variable chance of developing cancer progression over time, although these results are still questionable. Cyclin D1 controls the mitotic cell cycle's transition from G1 to S. Cyclin D1 has a significant function in carcinogenesis due to its critical involvement in cell cycle control. Cyclin D1 deregulation or overexpression may result in shortened G1 phase, enhanced cell replication, and decreased reliance on growth regulators, causing disruption in conventional cell cycle progression and the progression of cancer. The aim of the current study was to evaluate the expression of Cyclin D1 in histopathologically confirmed OLP samples and to predict the rate of malignant transformation in these lesions by comparing the level of expression with some clinical parameters. Materials and Methods: The immunohistochemical procedure was used to examine the expression of cyclin D1 on paraffin-embedded sections of (40) OLP lesions and (10) specimens of normal oral mucosa. We used the SPSS software version (18.0) to do the statistical analysis in this work. Results: There is a very significant difference between the people investigated (P = 0.01), which is statistically significant. CyclinD1 was found in all of the oral lichen planus cases in the research (100%), but it was also found (80%) in the normal oral mucosa. There was also no statistically significant difference between the clinical types of OLP and the Cyclin D1 scoring (P = 0.942), which revealed that the reticular type was the most frequent (7) in mild scoring, whereas the erosive type was the most frequent in severe scoring, which accounted for the majority of the cases. Conclusion: This research found that higher cyclinD1 expression increases the risk of cancer and many of the markers should be tested. Furthermore, larger sample numbers, improved designs, and gene-reduction studies should be performed. OLP patients should be continuously monitored for OSCC progression.

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“…Five of these had samples which were taken adjacent to OSCC samples, which are at risk of exhibiting a different genetic signature and possibly phenotype to traditional OLKs [17][18][19]76,77]. One was non-specific for keratin antibodies [78], and for another two, the methodology was not consistent with the aims of the paper (e.g., aiming to prove a new entity or validate a new technique [79,80]). Most of these studies found results similar to the trends found.…”

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confidence: 99%

Unraveling the Keratin Expression in Oral Leukoplakia: A Scoping Review

Murthy O,

Lau,

Balasubramaniam

et al. 2024

IJMS

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Intermediate filaments are one of three polymeric structures that form the cytoskeleton of epithelial cells. In the epithelium, these filaments are made up of a variety of keratin proteins. Intermediate filaments complete a wide range of functions in keratinocytes, including maintaining cell structure, cell growth, cell proliferation, cell migration, and more. Given that these functions are intimately associated with the carcinogenic process, and that hyperkeratinization is a quintessential feature of oral leukoplakias, the utility of keratins in oral leukoplakia is yet to be fully explored. This scoping review aims to outline the current knowledge founded on original studies on human tissues regarding the expression and utility of keratins as diagnostic, prognostic, and predictive biomarkers in oral leukoplakias. After using a search strategy developed for several scientific databases, namely, PubMed, Scopus, Web of Science, and OVID, 42 papers met the inclusion and exclusion criteria. One more article was added when it was identified through manually searching the list of references. The included papers were published between 1989 and 2024. Keratins 1–20 were investigated in the 43 included studies, and their expression was assessed in oral leukoplakia and dysplasia cases. Only five studies investigated the prognostic role of keratins in relation to malignant transformation. No studies evaluated keratins as a diagnostic adjunct or predictive tool. Evidence supports the idea that dysplasia disrupts the terminal differentiation pathway of primary keratins. Gain of keratin 17 expression and loss of keratin 13 were significantly observed in differentiated epithelial dysplasia. Also, the keratin 19 extension into suprabasal cells has been associated with the evolving features of dysplasia. The loss of keratin1/keratin 10 has been significantly associated with high-grade dysplasia. The prognostic value of cytokeratins has shown conflicting results, and further studies are required to ascertain their role in predicting the malignant transformation of oral leukoplakia.

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Significance of Expression of Cyclin D as an Early Indicator in Dysplastic Transformation of Oral Mucosa in Tobacco Users

Cited by 3 publications

References 19 publications

The expression of Cox-2 and Cyclin D1 with clinicopathological characteristics in oral epithelial dysplasia: A retrospective study

The expression of Cox-2 and Cyclin D1 with clinicopathological characteristics in oral epithelial dysplasia: A retrospective study

Immunohistochemical Expression of Cyclin D1 in Oral Lichen Planus

Unraveling the Keratin Expression in Oral Leukoplakia: A Scoping Review

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