Significance of fascin expression in laryngeal squamous cell carcinoma

Durmaz, Abdullah; Kurt, Bülent; O, Ongoru; Karahatay, Serdar; Gerek, Mustafa; Yalçın, Serenat Eriş

doi:10.1017/s0022215109991630

Cited by 19 publications

(23 citation statements)

References 23 publications

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“…We found a positive correlation between high expression of fascin-1 and advanced tumor stage (T3+T4), poor cancer differentiation, N+ cancer, and advanced clinical stage (III+IV). This observation was in line with the work of Durmaz et al [18] and Zou et al [19] for LSCC and other researchers for a number of other malignant neoplasms [12], [14].…”

Section: Discussionsupporting

confidence: 92%

“…Fascin-1 overexpression was found associated with unfavorable prognosis in many malignant tumors such as colon cancer [11], gastric cancer [13], oral squamous cell carcinoma [14],esophageal squamous cell carcinoma (ESCC) [15], non-small-cell lung cancer (NSCLC) [16], and breast cancer [17]. Overexpression of fascin-1 was found associated with aggressiveness of LSCC but in a limited number of subjects [18], [19].…”

Section: Introductionmentioning

confidence: 99%

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Fascin-1, Ezrin and Paxillin Contribute to the Malignant Progression and Are Predictors of Clinical Prognosis in Laryngeal Squamous Cell Carcinoma

et al. 2012

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AimsFascin-1, ezrin and paxillin, cytoskeleton-associated proteins, have been implicated in several human cancers, but their role in laryngeal squamous cell carcinoma (LSCC) is unknown. We investigated the association of their expression and clinicopathologic factors and their prognostic value in LSCC.Materials and MethodsQuantitative RT-PCR and western blot analyses were used to examine mRNA and protein levels in 10 fresh LSCC specimens and 10 corresponding adjacent normal margin (ANM) tissues from patients undergoing surgery in 2012. We used immunohistochemistry to retrospectively study 216 paraffin blocks of LSCC samples from patients (193 men) who had undergone surgery between 2000 and 2006 and had not received special treatment before the diagnosis. Univariate analysis of patient survival involved the Kaplan–Meier method. Multivariate analyses involved the Cox proportional hazards model.ResultsThe relative mRNA and protein levels of fascin-1, ezrin and paxillin were significantly greater in LSCC than ANM tissue (P<0.05). The high expression of fascin-1, ezrin or paxillin was positively correlated with poor tumor differentiation, cervical lymph node metastasis (N+), and advanced clinical stage (III+IV) (P<0.05) but not sex or metastasis. In addition, a high expression of fascin-1 (P = 0.007) or ezrin (P = 0.047) was associated with advanced tumor stage (T3+T4). The expression of fascin-1 was higher in smokers than non-smokers (P = 0.019). A high expression of fascin-1, ezrin or paxillin was associated with poor prognosis.ConclusionsFascin-1, ezrin and paxillin may be prognostic of poor outcome with LSCC after surgery. Our study may lead to establishing new molecular therapeutic targets and/or prognostic biomarkers in LSCC.

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Section: Discussionsupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Fascin-1, Ezrin and Paxillin Contribute to the Malignant Progression and Are Predictors of Clinical Prognosis in Laryngeal Squamous Cell Carcinoma

et al. 2012

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“…By contrast, it has been reported that fascin + tumor epithelium has strong invasiveness with poorer prognosis in various carcinomas, such as ovarian, breast, pancreas, lung, cutaneous, brain, gastrointestinal, thyroid, prostate, urothelial, intrahepatic cholangiocarcinoma and skin tumors (14)(15)(16)(17)(18)(19)(20)(21)(22)(23). The significance of epithelial fascin expression has been reported to be associated with invasiveness and metastasis, since it aids in cell migration and proliferation through increases in lamellipodial and filopodial cell protrusions (15).…”

Section: Epithelium -------------------------------------------------mentioning

confidence: 99%

“…In normal adult human tissues, fascin expression is restricted to neurons and DCs; both cell types have markedly large filopodia and are highly motile (11,13). Fascin expression in various neoplastic cells has also been investigated, and its expression has been reported to be associated with epithelial to mesenchymal progression and invasion of tumor cells as well as a clinically aggressive manifestation and poorer prognosis (14)(15)(16)(17)(18)(19)(20)(21)(22)(23). However, to the best of our knowledge, immunohistochemical studies for the detection of fascin + mature DCs in thymoma have yet to be performed.…”

Section: Introductionmentioning

confidence: 99%

Fascin expression in dendritic cells and tumor epithelium in thymoma and thymic carcinoma

et al. 2011

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Abstract. The majority of thymomas are histologically characterized by tumor-infiltrating lymphocytes. Mature dendritic cells (DCs) are known to assemble lymphocytes through antigen presentation to T lymphocytes. Fascin, a 55-kDa actin-binding protein and a known marker for mature DCs, regulates filaments necessary for the formation of filopodia in cell migration. Moreover, fascin expression in various epithelial neoplasms has recently been reported to be associated with invasion of tumor cells and clinically aggressive manifestations. In the present study, we investigated fascin expression immunohistochemically in tissues of thymomas and thymic carcinomas surgically resected at our institute. A total of 34 thymomas and 5 thymic carcinomas were included. The amount and immunohistochemical intensity of both fascin + DCs and tumor epithelium were counted and assessed, and the clinicopathological data were also scored. Statistical analyses revealed that the amount of fascin + DCs with the formation of clusters was associated with lymphocyte-rich variants (p=0.002) and cortical differentiation (p=0.037) of thymoma with complication from myasthenia gravis (p=0.002). The quantity of fascin + epithelium was associated with a strong intensity of fascin in infiltrating DCs (p=0.002) with the formation of clusters (p=0.002) and favorable prognosis, as assessed by the Masaoka staging system (p=0.001). The amount of infiltrating DCs (p=0.024) and fascin + epithelium were lower in thymic carcinoma. It was concluded that fascin + epithelium may induce tumor immunity through the surveillance activity of fascin + DCs in thymic neoplasms, thus improving prognosis.

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“…Calponin may be a prognostic factor of laryngeal myoepithelial carcinoma [3]. Other proteins are also related to the progress of laryngeal carcinoma, such as REPP86 [4], DJ-1 [5], SATB1 [6], CRBP-1 [7], fascin [8] and Cap-43 [9]. However, few studies were performed on a systematic protein or gene level to explore the roles of these proteins in different laryngeal carcinoma progress.…”

Section: Introductionmentioning

confidence: 99%

Preliminary proteomic analysis of human serum from patients with laryngeal carcinoma

Zhang

Song

Liu

et al. 2011

Eur Arch Otorhinolaryngol

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To better evaluate the serum protein alterations in patients with laryngeal carcinoma during surgical treatment process, a comparative proteomic analysis of human serum from patients with laryngeal carcinoma between pre- and post-operation group was performed using two-dimensional gel electrophoresis, in combination with matrix-assisted laser desorption ionization time-of-flight mass spectrometry and database searching. Statistical analysis indicated that 16 gel spots corresponding to 12 proteins altered their expression significantly between the two groups of patients with laryngeal carcinoma. Of these 12 proteins, 6 proteins were up-regulated in the pre-operation group. The subsequent Western blot confirmed the results of proteome analysis. Measurement of MDA and SOD levels in serum combining with bioinformatics analysis indicated the potential roles of the oxidant stress and immune response as target in monitoring and treating laryngeal carcinoma. The study provides new insight into the laryngeal carcinoma development and treatment, and the identified proteins are warranted to be further studied.

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Significance of fascin expression in laryngeal squamous cell carcinoma

Abstract: These results suggest that laryngeal squamous cell carcinomas with high levels of fascin expression may be more aggressive than those with low expression levels. Further studies with larger series are needed to support these results and to clarify rationales.

Cited by 19 publications

References 23 publications

Fascin-1, Ezrin and Paxillin Contribute to the Malignant Progression and Are Predictors of Clinical Prognosis in Laryngeal Squamous Cell Carcinoma

Fascin-1, Ezrin and Paxillin Contribute to the Malignant Progression and Are Predictors of Clinical Prognosis in Laryngeal Squamous Cell Carcinoma

Fascin expression in dendritic cells and tumor epithelium in thymoma and thymic carcinoma

Preliminary proteomic analysis of human serum from patients with laryngeal carcinoma

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