Significance of PYK2 level as a prognosis predictor in patients with colon adenocarcinoma after surgical resection

Liu, Shufang; Xu, Yaping

doi:10.2147/ott.s169531

Cited by 11 publications

(7 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…During the past decades, many efforts have been made to search for biomarkers of COAD. Studies have shown the diagnostic value of DCTN1, DCTN2, and DCTN4 [17] and the prognostic role of LAYN [18], KCNQ1OT1 [19], and PYK2 [20] in COAD, but with limited sample size or inconsistent results. In this study, we analyzed the expression patterns of m 6 A RNA methylation regulators in COAD and their relationship with clinical features by using cohort data from TCGA.…”

Section: Discussionmentioning

confidence: 99%

The YTH Domain Family of N6-Methyladenosine “Readers” in the Diagnosis and Prognosis of Colonic Adenocarcinoma

Shao

Song

et al. 2020

BioMed Research International

View full text Add to dashboard Cite

To profile the landscape of methylation N6 adenosine (m6A) RNA regulators in colonic adenocarcinoma (COAD) and to explore potential diagnostic and prognostic biomarkers, we assessed the differential expression patterns of m6A RNA methylation regulators between 418 COAD patients and 41 controls based on profiling from The Cancer Genome Atlas (TCGA) database. We plotted the receiver operating characteristic (ROC) curves and calculated the area under the curve (AUC) values to estimate the discrimination ability. The relationship between the expression of m6A RNA methylation regulators and clinicopathological characteristics was explored. Kaplan-Meier plotter, log-rank test, and Cox regression were used and a nomogram was created to explore the prognostic significance of m6A-related genes in overall survival at the mRNA level. Pathway analysis was performed by gene set enrichment analysis (GSEA) using TCGA dataset, and a coexpression network was built based on the STRING database. We observed that YTHDF1, METTL3, and KIAA1429 were significantly upregulated, while YTHDF3, YTHDC2, METTL14, and ALKBH5 were significantly downregulated in COAD samples compared to normal samples. YTHDF1 had the highest diagnostic value. Low expression of YTHDF3 predicted a poor survival rate in COAD patients. YTHDC2 was related to sex and showed a downward trend as clinical stage increased. Our results indicate that the YT521-B homology (YTH) domain family (“readers”), especially YTHDF1, YTHDF3, and YTHDC2, might play a significant role in the detection, progression, and prognosis of COAD, indicating that they are promising cancer biomarkers.

show abstract

Section: Discussionmentioning

confidence: 99%

The YTH Domain Family of N6-Methyladenosine “Readers” in the Diagnosis and Prognosis of Colonic Adenocarcinoma

Shao

Song

et al. 2020

BioMed Research International

View full text Add to dashboard Cite

show abstract

“…Activation of PYK2 involves autophosphorylation at Tyr-402, which enables the binding of Src via the SH2 domain and phosphorylation of PYK2 at Tyr-579 and Tyr-580, within the kinase domain activation loop, to generate maximal kinase activity (22). Phosphorylated PYK2 functions in the regulation of phagocytosis, migration, proliferation, invasion, oncogenesis and metastasis (21,(23)(24)(25). In macrophages, PYK2 has defined functions in regulating morphology, migration, and phagocytosis (21,26,27).…”

Section: Introductionmentioning

confidence: 99%

Inhibition of proline tyrosine kinase 2 (Pyk2) phosphorylation during adherent-invasiveEscherichia coliinfection inhibits intra-macrophage replication and inflammatory cytokine release

Li¹,

Ormsby²,

Fallata³

et al. 2022

Preprint

View full text Add to dashboard Cite

Adherent-invasive Escherichia coli (AIEC) have been implicated in the aetiology of Crohns Disease (CD). They are characterized by an ability to adhere to and invade intestinal epithelial cells, and to replicate intracellularly in macrophages resulting in inflammation. Proline-rich tyrosine kinase 2 (PYK2) has previously been identified as a risk locus for inflammatory bowel disease through genome wide association studies. It is overexpressed in patients with colorectal cancer, a major long-term complication of CD. Here we show that Pyk2 levels are significantly increased during AIEC infection of murine macrophages while an inhibitor of Pyk2, PF-431396 hydrate, significantly decreased intramacrophage AIEC numbers. Imaging flow cytometry indicated that Pyk2 inhibition blocked intramacrophage replication of AIEC with no change in the overall number of infected cells, but a significant reduction in bacterial burden per cell. This reduction in intracellular bacteria resulted in a 20-fold decrease in tumour necrosis factor alpha secretion by cells post-AIEC infection. These data demonstrate a key role for Pyk2 in modulating AIEC intracellular replication and associated inflammation and may provide a new avenue for future therapeutic intervention in CD.

show abstract

“…PYK2 consists of three domains associated with tumor progression and can be activated by multiple growth factors, neuropeptides, cytokines, hormones, and chemokines [10][11][12][13][14][15]. Overexpression of PYK2 has been reported to promote progression and lead to poor prognosis in many kinds of malignant tumors such as breast cancer, liver cancer, lung cancer and pancreatic cancer [7,[16][17][18][19][20][21][22]. PYK2 was only found to serve as an onco-suppressor gene in androgen-dependent prostate cancer cells and neuroblastoma cells so far [23,24].…”

Section: Introductionmentioning

confidence: 99%

High Expression of PYK2 is Associated with Poor Prognosis and Cancer Progression in Early- Stage Cervical Carcinoma

Zhang

Zhu

Yong

et al. 2021

Preprint

View full text Add to dashboard Cite

Background: Proline-rich tyrosine kinase-2 (PYK2), also known as calcium dependent tyrosine kinase (CADTK), regulates different signal transduction cascades that control cell proliferation, migration and invasion. However, the role of PYK2 in cervical cancer remains to be elucidated.Methods: PYK2 expression was detected in tissue microarray and cervical cancer cell lines. Statistical analysis was performed to evaluate its clinicopathological significance. Small interfering RNA (SiRNA) was employed to suppress endogenous PYK2 expression in cervical cancer cells to observe the biological function.Results: PYK2 expression was up-regulated in cervical cancer specimens compared with paired adjacent normal cervical tissue samples. Statistical analyses indicated that PYK2 expression might be an independent prognostic indicator for patients with early-stage cervical cancer. A nomogram model was constructed based on PYK2 expression and other clinicopathological risk factors, and it performed well in predicting patients survival. In cellular studies, down-regulation of PYK2 remarkably inhibited cellular proliferation, migration and invasion.Conclusion: PYK2 expression possessed the potential to serve as a novel prognostic markerin cervical cancer patients.

show abstract

Significance of PYK2 level as a prognosis predictor in patients with colon adenocarcinoma after surgical resection

Cited by 11 publications

References 45 publications

The YTH Domain Family of N6-Methyladenosine “Readers” in the Diagnosis and Prognosis of Colonic Adenocarcinoma

The YTH Domain Family of N6-Methyladenosine “Readers” in the Diagnosis and Prognosis of Colonic Adenocarcinoma

Inhibition of proline tyrosine kinase 2 (Pyk2) phosphorylation during adherent-invasiveEscherichia coliinfection inhibits intra-macrophage replication and inflammatory cytokine release

High Expression of PYK2 is Associated with Poor Prognosis and Cancer Progression in Early- Stage Cervical Carcinoma

Contact Info

Product

Resources

About