Significance of the chemokine CXCL10 and human beta-defensin-3 as biomarkers of pulmonary tuberculosis

Ali, Zainab; Mankhi, Ahmed A.; Ad’hiah, Ali H.

doi:10.1016/j.tube.2021.102078

Cited by 9 publications

(7 citation statements)

References 45 publications

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“…Among them, the relationship between CXCL10 and TB has been widely concerned [ 25 , 26 ]. Many scholars believe that CXCL10 is an important biomarker of TB [ 27 ]; this is consistent with our research results. However, what is more noteworthy in this study is that in TB patients' peripheral blood, IL-27 and CXCL10 have a certain correlation ( r = 0.68, P < 0.05).…”

Section: Discussionsupporting

confidence: 93%

Study on the Correlation between Interleukin-27 and CXCL10 in Pulmonary Tuberculosis

Fan

Yang

Wang

et al. 2022

Journal of Immunology Research

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Objective. To investigate the correlation between interleukin-27 and CXCL10 and other cytokines in pulmonary tuberculosis and to further explore the related miRNAs through bioinformatics. Methods. Collect the lesion tissue and peripheral blood of pulmonary tuberculosis patients and the peripheral blood of healthy controls. Immunohistochemical staining and qRT-PCR were used to observe the expression of interleukin-27, CXCL9, CXCL10, and CXCL11. Then, predict the key miRNA, qRT-PCR was used to verify the expression of miRNA in the peripheral blood and evaluated the correlation between them. Results. Both immunohistochemical staining and qRT-PCR indicated that the expressions of IL-27, CXCL9, CXCL10, and CXCL11 were significantly increased in tuberculosis patients, and IL-27 was significantly correlated with CXCL10 ( r = 0.68 ). Key molecules such as has-let-7b-5p, has-miR-30a-3p, and has-miR-320b were screened out. Among them, has-let-7b-5p was significantly downregulated, and has-miR-30a-3p was significantly upregulated; they were related to interleukin-27 and CXCL10. Conclusion. Our data shows that interleukin-27 and CXCL10 are significantly related in pulmonary tuberculosis, and has-let-7b-5p and has-miR-30a-3p are also related to interleukin-27 and CXCL10. It laid the foundation for subsequently exploiting the potential biomarkers in tuberculosis disease.

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Section: Discussionsupporting

confidence: 93%

Study on the Correlation between Interleukin-27 and CXCL10 in Pulmonary Tuberculosis

Fan

Yang

Wang

et al. 2022

Journal of Immunology Research

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“…In line with above previous studies, we found there is a significant difference (p value=0.028) in the level of this Marker (CXCL9) between new patients and healthy people in our findings, and this matches with the previous study [9]. However, it in the term of CXCL9 level in tuberculosis patients before and after treatment, there was a slight decreasing in the serum level of this biomarker but did not reach a significant level and the reason may be the small sample size of under treatment TB patients' number (n=30).…”

Section: A the Importance Of Estimating The Level Of Inflammatory Mar...supporting

confidence: 94%

Potential Immunological Biomarker for Diagnosis and Prognosis of Tuberculosis

Hussain

Mohammed²,

Ali³

2023

EJBIOMED

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Tuberculosis (TB) is one of the most common infectious diseases in the world, which has led to numerous deaths. Hence, developing an efficient diagnostic method is essential to monitor and control such deadly infectious diseases. In the current study, the serum levels of four inflammatory markers (CXCL10, CXCL9, suPAR, and MMP9) and the expression NF-κB gene were evaluated as potential immunological markers for diagnosis and prognosis of tuberculosis, using ELISA and qPCR technique respectively. Thirty new TB patients and equal numbers of under treatment TB patients and control (healthy people) were conscripted in this study. The results showed significant differences in the serum level of CXCL10 among the three groups (p value 0.003) and between new and under treatment patients (P value 0.004). A significant difference in the CXCL9 level in the serum was observed between the new TB patients and the healthy group with p value 0.028 but didn’t reach the significant level between the new and under treatment patients. The serum level of suPAR was higher in new patients (106.59pg/ml) followed by treated patients (89.66pg/ml) and lowest in healthy group (80.71pg/ml) but didn’t reach the significant level. Also, the serum level of MMP-9 did not show a significant difference between the tested groups, but it was slightly higher in new patients (21.45ng/ml) compared to the healthy group (20.70ng/ml). The amount of NF-κB gene expression was significantly higher in new patients (8.21-fold change) than in under treatment patients (2.95-fold change) in comparing with healthy people.

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“…The present study focused on four HBDs (HBD1, HBD2, HBD3, and HBD4), which represent an important class of innate immune modulators that act non-specifically against microbial challenges (12). The systemic profile of HBDs has been extensively studied in infectious, autoimmune, and inflammatory diseases, and dysregulated production has been associated with disease progression (18)(19)(20). In the case of meningitis, the CSF profile of HBDs is the least studied and there have been limited data assessing their role in the development of meningitis.…”

Section: Discussionmentioning

confidence: 99%

“…The most widely studied HBDs are HBD1, HBD2, HBD3, and HBD4 (15,16). Recent evidence indicates that in addition to being components of innate antimicrobial immunity, HBDs also play a key role as pro-inflammatory mediators and immunostimulators that increase the response to infection (18)(19)(20). In meningitis, HBDs have not been well investigated, but their roles in neuroimmune function and neurodegeneration have been proposed (21).…”

Section: Evaluation Of Human β-Defensins In the Cerebrospinal Fluid O...mentioning

confidence: 99%

Evaluation of human β‑defensins in the cerebrospinal fluid of suspected meningitis

Jaleel

Umran

Kaddo³

et al. 2022

Biomed Rep

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Human β-defensins (HBDs) are an important class of antimicrobial peptides that have immunomodulatory functions; however, the role of HBDs have not been well explored in the pathogenesis of meningitis. A cross-sectional study was performed to explore the levels of HBD1, HBD2, HBD3, and HBD4 in the cerebrospinal fluid (CSF) of 176 suspected meningitis cases. CSF samples were first subjected to PCR analysis using a set of universal primers targeting a portion of the eubacteria 16S rRNA gene. The analysis demonstrated that 66 samples (37.5%) were PCR-positive, whilst 110 samples (62.5%) were PCR-negative. DNA sequence analysis of the PCR-positive products identified two broad categories of bacteria, Gram-negative (68.2%) and Gram-positive (31.8%). A total of 88 PCR-negative CSF samples showed abnormal leukocyte counts, glucose concentrations, and/or protein concentrations, and were considered abnormal (ABN). The remaining 22 CSF samples were considered normal (NOR). HBD1, HBD2, and HBD4 levels did not exhibit significant differences between PCR-positive, ABN, and NOR CSF samples. However, HBD3 levels were significantly higher in the ABN CSF samples than in the NOR CSF samples (P=0.005). HBD3 levels were also elevated in the PCR-positive CSF samples compared with the NOR CSF samples, but the difference was not significant (P= 0.151). HBD2, HBD3, and HBD4 were correlated with leukocyte counts, glucose concentration, and protein concentration. In conclusion, HBD3 levels were significantly elevated in the CSF of suspected meningitis cases regardless of the cause of meningitis. The CSF levels of certain HBDs were affected by specific diagnostic laboratory parameters for meningitis, including leukocyte counts, glucose concentration, and protein concentration.

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Significance of the chemokine CXCL10 and human beta-defensin-3 as biomarkers of pulmonary tuberculosis

Cited by 9 publications

References 45 publications

Study on the Correlation between Interleukin-27 and CXCL10 in Pulmonary Tuberculosis

Study on the Correlation between Interleukin-27 and CXCL10 in Pulmonary Tuberculosis

Potential Immunological Biomarker for Diagnosis and Prognosis of Tuberculosis

Evaluation of human β‑defensins in the cerebrospinal fluid of suspected meningitis

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