In this present work, a review of fatigue strength on bio‐nano composites was presented. Generally, the biocomposites possessed higher fatigue strength than the conventional materials. Because the propagation in biocomposites can be arrested due to their internal structure, whereas the damages occur in the matrix and/or at the fiber/matrix interface region. In the case of conventional materials, the cracks would rapidly grow until a catastrophic failure occurs. Thus, the S–N curves of composites show a flatter curve when compared to the conventional materials. In terms of fatigue properties, conventional fiber‐reinforced composites (e.g., glass and carbon‐reinforced composites) were more extensively studied than bio‐based reinforced composites. Examining the fatigue properties of materials is significant for all engineering‐based applications. Because the fatigue failures can occur below the ultimate tensile strength of materials. However, the fatigue performance of the composites can be enhanced by improving the fiber‐matrix interfacial bonding resulting from surface treatment of fibers and incorporating nanofillers within the matrix, and so forth. Since the nanocomposites have a larger surface area and aspect ratio, they are preferred to use in many applications: aerospace, automotive, biotechnology, construction, and building, electronics, marine, and packing industries. Thus, this chapter starts with the unique advantages of bio‐nano composites. Then, the fatigue properties of bio‐nano composites, the significance of the S–N diagram, and the pattern of fatigue testing were discussed. In order to understand the fatigue behavior, several factors such as structure, fillers, fabrication techniques, and so forth were included. Challenges of fatigue strength of biocomposites were also summarized.